Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,297,668	C→T	D567N (GAC→AAC)	fdhF ←	formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,297,668	0	C	T	100.0%	110.2 / NA	35	D567N (GAC→AAC)	fdhF	formate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/16);  total (19/16)

TGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAAT  >  NC_000913/4297543‑4297791                                                                                                                              |                                                                                                                            tgCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTAcc                                                                                                                >  1:530331/1‑139 (MQ=255)        ccAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGc                                                                                                         <  1:260160/139‑1 (MQ=255)             ttCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTgg                                                                                                                       >  2:93714/1‑120 (MQ=255)             ttCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTgg                                                                                                                       <  1:93714/120‑1 (MQ=255)                           ccgccAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAg                                                                                      <  2:699134/139‑1 (MQ=255)                              ccAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCCCCAGAcc                                                                                   >  1:717305/1‑139 (MQ=255)                                   gCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCg                                                                              <  2:325460/139‑1 (MQ=255)                                            ttACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCaa                                                                     >  2:480764/1‑139 (MQ=255)                                               ccGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                  <  2:530331/139‑1 (MQ=255)                                               ccGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                  <  1:393297/139‑1 (MQ=255)                                               ccGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                  <  1:112411/139‑1 (MQ=255)                                               ccGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACt                                                                  <  1:792706/139‑1 (MQ=255)                                                      tCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCttt                                                           >  2:695099/1‑139 (MQ=255)                                                             gCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGa                                                    >  2:636743/1‑139 (MQ=255)                                                              cAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGAt                                                   <  1:636743/139‑1 (MQ=255)                                                                 gagTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaag                                                <  1:695099/139‑1 (MQ=255)                                                                       tGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTcc                                          >  1:229056/1‑139 (MQ=255)                                                                              cTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaagaa                                              <  1:338293/128‑1 (MQ=255)                                                                              cTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATaagaa                                              >  2:338293/1‑128 (MQ=255)                                                                              cTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                   >  1:748885/1‑139 (MQ=255)                                                                              cTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATc                                   >  1:252892/1‑139 (MQ=255)                                                                                   cGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGc                                                                                                         >  1:726152/1‑64 (MQ=255)                                                                                   cGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGc                                                                                                         <  2:726152/64‑1 (MQ=255)                                                                                        cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGc                                                                                                         >  2:121744/1‑59 (MQ=255)                                                                                        cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGc                                                                                                         <  1:121744/59‑1 (MQ=255)                                                                                        cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGaa                         >  1:111425/1‑139 (MQ=255)                                                                                        cGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTAACCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATAGGCATCTGaa                         >  1:99259/1‑139 (MQ=255)                                                                                                  aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                                >  2:190827/1‑122 (MQ=255)                                                                                                  aCCATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGc                                <  1:190827/122‑1 (MQ=255)                                                                                                    cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTaaa                                                        >  2:406297/1‑96 (MQ=255)                                                                                                    cATCGGGTACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTaaa                                                        <  1:406297/96‑1 (MQ=255)                                                                                                           tACTCGTCGGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACt      >  1:301205/1‑139 (MQ=255)                                                                                                              tcgtcgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                               <  2:106519/47‑1 (MQ=255)                                                                                                              tcgtcgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTgaagaa                                                                                               >  1:106519/1‑47 (MQ=255)                                                                                                               cgtcgGTGAGTTTGTTGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACtgaat  >  2:766202/1‑139 (MQ=255)                                                                                                                              |                                                                                                                            TGCGTAGCCAGGTTCATCAGCCAGCGCCGCCAGTGCCGCACAGTTACCGGTCATCGAACGGCAAGAGTAGTGACCAACTTCACGCACCGTTGACAGTACCATCGGGTACTCGTCGGTGAGTTTGTCGATTGGCGCTACCCAGTCGCAGGTGAAGAACTGCGCCAGACCGTTCGGGGTATCAAACTTCTCTTTAAACAGATAAGAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAAT  >  NC_000913/4297543‑4297791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑