Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	35,373	G→C	intergenic (‑2/+4)	caiE ← / ← caiD	stimulator of CaiD and CaiB enzyme activities/carnitinyl‑CoA dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	35,373	0	G	C	100.0%	111.6 / NA	31	intergenic (‑2/+4)	caiE/caiD	stimulator of CaiD and CaiB enzyme activities/carnitinyl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/15);  total (16/15)

GCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATAC  >  NC_000913/35242‑35497                                                                                                                                    |                                                                                                                             gCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTaa                                                                                                                       >  1:727345/1‑139 (MQ=255)  cAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAAc                                                                                                                      <  1:212106/139‑1 (MQ=255)       aGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGCATGGACAAACGCCGTCGGGTGAACCACCGGCATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCt                                                                                                                 >  1:523638/1‑139 (MQ=255)               gggCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAAc                                                                                                                      >  1:241939/1‑126 (MQ=255)               gggCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAAc                                                                                                                      <  2:241939/126‑1 (MQ=255)               gggCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCacac                                                                                                         >  1:478089/1‑139 (MQ=255)                           acacCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCtt                                                                                             <  1:718241/139‑1 (MQ=255)                                               aTCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGcc                                                                         >  1:472655/1‑139 (MQ=255)                                                   ccAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTc                                                                     >  2:326802/1‑139 (MQ=255)                                                   ccAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTc                                                                     >  1:346166/1‑139 (MQ=255)                                                         aaGACGGCACTGGGATGGACAGACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGc                                                               <  2:471985/139‑1 (MQ=255)                                                           gACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCAt                                                             <  2:88484/139‑1 (MQ=255)                                                                 aCTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATgg                                                                <  1:764296/130‑1 (MQ=255)                                                                 aCTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATgg                                                                >  2:764296/1‑130 (MQ=255)                                                                        tGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAg                                                <  2:494917/139‑1 (MQ=255)                                                                          gACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGaa                                              >  2:369753/1‑139 (MQ=255)                                                                               aCGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGAt                                         >  1:564375/1‑139 (MQ=255)                                                                                    gTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATa                                    <  2:647350/139‑1 (MQ=255)                                                                                     tCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAg                                   <  2:6436/139‑1 (MQ=255)                                                                                                 ccGGAATTAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACAcg                       <  2:727345/139‑1 (MQ=255)                                                                                                        tAACCCCTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGc                <  2:346166/139‑1 (MQ=255)                                                                                                            cccTCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAat            >  1:534880/1‑139 (MQ=255)                                                                                                               tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGttt                              <  2:264847/118‑1 (MQ=255)                                                                                                               tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGttt                              >  1:264847/1‑118 (MQ=255)                                                                                                               tCAAAGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAAtata         >  1:672362/1‑139 (MQ=255)                                                                                                                 aaaGGCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACAACGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGc       >  2:209356/1‑139 (MQ=255)                                                                                                                    ggCGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGAt    <  1:369753/139‑1 (MQ=255)                                                                                                                      cGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATAc  >  2:569637/1‑139 (MQ=255)                                                                                                                      cGTAATAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATAc  <  2:478089/139‑1 (MQ=255)                                                                                                                           tAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCa                                                                    <  2:216036/68‑1 (MQ=255)                                                                                                                           tAGCTCACGCTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCa                                                                    >  1:216036/1‑68 (MQ=255)                                                                                                                                    |                                                                                                                             GCAGTGAGGCGAGTGGGCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATAC  >  NC_000913/35242‑35497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑