Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,489,643 C→T A141T (GCT→ACT)  yjgR ← DUF853 family protein with NTPase fold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,489,6430CT97.2% 109.1 / NA 36A141T (GCT→ACT) yjgRDUF853 family protein with NTPase fold
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/16);  minor base A (1/0);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGATGGCACCAACCGATGCGCTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCAC  >  NC_000913/4489511‑4489773
                                                                                                                                    |                                                                                                                                  
gcTGGATGGCACCAACCGATGCGCTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCg                                                                                                                              <  1:685969/139‑1 (MQ=255)
              aCCGATGCGCTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCa                                                                                                                >  2:616992/1‑139 (MQ=255)
              aCCGATGCGCTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCa                                                                                                                >  1:551739/1‑139 (MQ=255)
               ccGATGCGCTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAg                                                                                                               <  2:238368/139‑1 (MQ=255)
                          cTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGAACTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGAtt                                                                                                    >  2:623454/1‑139 (MQ=255)
                              tagtACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGcac                                                                                                <  2:452778/136‑1 (MQ=255)
                               atTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGcaca                                                                                               <  2:624633/139‑1 (MQ=255)
                                  aCCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCg                                                                                            >  1:758922/1‑139 (MQ=255)
                                    cGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGtt                                                                                          >  2:376273/1‑139 (MQ=255)
                                    cGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGtt                                                                                          >  1:327939/1‑139 (MQ=255)
                                        cTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGTTCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGCCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTgaga                                                                                      >  2:583159/1‑139 (MQ=255)
                                           aTTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTc                                                                                   >  2:576206/1‑139 (MQ=255)
                                           aTTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTc                                                                                   >  2:481710/1‑139 (MQ=255)
                                                  aaGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTc                                                                            <  1:158290/139‑1 (MQ=255)
                                                              ttATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACa                                                                <  1:583159/139‑1 (MQ=255)
                                                                      gATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGcccc                                                        <  2:551739/139‑1 (MQ=255)
                                                                              gggTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGa                                                <  2:758922/139‑1 (MQ=255)
                                                                                    ttGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGt                                          >  1:494500/1‑139 (MQ=255)
                                                                                    ttGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGt                                          >  1:229712/1‑139 (MQ=255)
                                                                                      gCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCg                                        >  2:79061/1‑139 (MQ=255)
                                                                                           cAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTg                                                                                         >  2:272986/1‑85 (MQ=255)
                                                                                           cAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTg                                                                                         <  1:272986/85‑1 (MQ=255)
                                                                                                      aGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCa                                                       >  2:331695/1‑108 (MQ=255)
                                                                                                      aGTCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCa                                                       <  1:331695/108‑1 (MQ=255)
                                                                                                        tCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGGTTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGc                                       <  1:523227/122‑1 (MQ=255)
                                                                                                        tCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGc                                       >  2:523227/1‑122 (MQ=255)
                                                                                                        tCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGc                                       >  2:554606/1‑122 (MQ=255)
                                                                                                        tCGAGCAGTAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGc                                       <  1:554606/122‑1 (MQ=255)
                                                                                                          gAGCAGTCACAGCCCCTGCTCGCCAGAAATGCGGAAGATGATATTCAGCACGCTAGATTGCACCTCGTTGACATTCAACAGTCGTGCGCGCGACACCGGCCCCAGATCCGAAACCGTCGCCCTCACCGGATGGCCTTtc                    >  1:412726/1‑139 (MQ=255)
                                                                                                                tAACAGCCCCTGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCGACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCa              <  2:327939/139‑1 (MQ=255)
                                                                                                                          tGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGca                                                                                                 <  1:800627/46‑1 (MQ=255)
                                                                                                                          tGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGca                                                                                                 >  2:800627/1‑46 (MQ=255)
                                                                                                                          tGATCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATccc    >  1:442210/1‑139 (MQ=255)
                                                                                                                            aTCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCgg                                                            <  1:743045/81‑1 (MQ=255)
                                                                                                                            aTCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCgg                                                            >  2:743045/1‑81 (MQ=255)
                                                                                                                            aTCGTCAGTAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCcac  <  1:627306/139‑1 (MQ=255)
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GCTGGATGGCACCAACCGATGCGCTACTGATATTACCGTACTGATTCTGGAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCAC  >  NC_000913/4489511‑4489773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑