Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,493,416 C→T G331S (GGT→AGT)  idnD ← L‑idonate 5‑dehydrogenase, NAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,493,4160CT100.0% 139.1 / NA 44G331S (GGT→AGT) idnDL‑idonate 5‑dehydrogenase, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/20);  total (24/20)

CCTGTGCTGAACCGGTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGA  >  NC_000913/4493294‑4493550
                                                                                                                          |                                                                                                                                      
ccTGTGCTGAACCGGTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGcc                                                                                                                        <  1:744698/139‑1 (MQ=255)
        gAACCGGTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGACACGTAGCGCCTCTTCCAg                                                                                                                <  1:445370/139‑1 (MQ=255)
         aaCCGGTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAgg                                                                                                               >  2:555856/1‑139 (MQ=255)
              gTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGt                                                                                                              >  1:325593/1‑135 (MQ=255)
              gTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGt                                                                                                              <  2:325593/135‑1 (MQ=255)
              gTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGt                                                                                                          >  2:245665/1‑139 (MQ=255)
              gTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGt                                                                                                          >  2:180769/1‑139 (MQ=255)
              gTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGt                                                                                                          >  1:343228/1‑139 (MQ=255)
               tAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTg                                                                                                         <  1:245665/139‑1 (MQ=255)
                         atTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGAATTATTCCTTTATTTAGCTCTTAGAAAACAAGCTTGACTTTGTCTGCCTGGATTTTATCACTGGCGAAGCGTAGCGCCTCTTCCAGGTCAGTGAAGGGATAtt                                                                                               <  1:37508/139‑1 (MQ=255)
                              ttCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTc                                                                                                                   >  2:602984/1‑114 (MQ=255)
                              ttCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTc                                                                                                                   <  1:602984/114‑1 (MQ=255)
                               tCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCAc                                                                                         <  2:343228/139‑1 (MQ=255)
                                                      tCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATa                                                                  >  2:319753/1‑139 (MQ=255)
                                                          tGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCAc                                                                                         <  2:747706/112‑1 (MQ=255)
                                                          tGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCAc                                                                                         >  1:747706/1‑112 (MQ=255)
                                                            tattatTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGcc                                                                                                                        >  2:382289/1‑79 (MQ=255)
                                                            tattatTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGcc                                                                                                                        <  1:382289/79‑1 (MQ=255)
                                                               tattCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTc                                                         >  1:60309/1‑139 (MQ=255)
                                                                 ttCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCa                                                                                          <  1:390550/104‑1 (MQ=255)
                                                                 ttCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCa                                                                                          >  2:390550/1‑104 (MQ=255)
                                                                 ttCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgc                                                       >  1:745172/1‑139 (MQ=255)
                                                                 ttCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCGGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgc                                                       >  1:569013/1‑139 (MQ=255)
                                                                  tCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCGCTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgcc                                                      <  1:221792/139‑1 (MQ=255)
                                                                  tCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCAc                                                                                         >  2:112958/1‑104 (MQ=255)
                                                                  tCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCAc                                                                                         <  1:112958/104‑1 (MQ=255)
                                                                    cTTTATTTATCTCTTAGAACACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGt                                                                                                              <  2:227216/81‑1 (MQ=255)
                                                                    cTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGt                                                                                                              >  1:227216/1‑81 (MQ=255)
                                                                    cTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccag                                                    >  1:668055/1‑139 (MQ=255)
                                                                      ttatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGcc                                                                                                                        <  1:717597/69‑1 (MQ=255)
                                                                      ttatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGcc                                                                                                                        >  2:717597/1‑69 (MQ=255)
                                                                      ttatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccagcc                                                  >  2:119621/1‑139 (MQ=255)
                                                                      ttatttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCgccagcc                                                  >  1:311557/1‑139 (MQ=255)
                                                                        atttatCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCAt                                                <  2:668055/139‑1 (MQ=255)
                                                                              ctctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACt                                          >  1:14920/1‑139 (MQ=255)
                                                                              ctctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACt                                          >  1:324/1‑139 (MQ=255)
                                                                               tctTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTg                                         <  2:14920/139‑1 (MQ=255)
                                                                                 ttAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTc                                                                                                                   <  2:150097/63‑1 (MQ=255)
                                                                                 ttAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTc                                                                                                                   >  1:150097/1‑63 (MQ=255)
                                                                                                      tgctgcCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTaaaa                  >  1:791580/1‑139 (MQ=255)
                                                                                                          gcCTGGGTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCTGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGa              <  1:180769/139‑1 (MQ=255)
                                                                                                               ggTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAg                                                                            >  1:780720/1‑72 (MQ=255)
                                                                                                               ggTTTTATCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAg                                                                            <  2:780720/72‑1 (MQ=255)
                                                                                                                      tCACTGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGa  <  2:60309/139‑1 (MQ=255)
                                                                                                                          |                                                                                                                                      
CCTGTGCTGAACCGGTAATCAAGATATTTTTTCCTGCCAGTGAAAATAGATCGTTCATTGTATTATTCCTTTATTTATCTCTTAGAAAACAAGCTGGACTTTTGCTGCCTGGGTTTTATCACCGGCGAAACGTAGCGCCTCTTCCAGGTCAGTGAAGGGATATTCAGCACTCAGTAAAGGCAGTGGATTGATAACGCCATTCGCCAGCCATGACACTGCGGTATTAAATTCGCTGGTAAAACGGAAAGAGCCTCTGA  >  NC_000913/4493294‑4493550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑