Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,120	G→A	*475* (TAG→TAA)	creC →	sensory histidine kinase in two‑component regulatory system with CreB or PhoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,120	0	G	A	100.0%	105.2 / NA	34	*475* (TAG→TAA)	creC	sensory histidine kinase in two‑component regulatory system with CreB or PhoB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/18);  total (16/18)

CAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCG  >  NC_000913/4637989‑4638246                                                                                                                                    |                                                                                                                               cAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCaaa                                                                                                                         <  1:747541/139‑1 (MQ=255)            cGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCcaca                                                                                                              <  2:757040/139‑1 (MQ=255)                        ggCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCca                                                                                                                >  1:691980/1‑125 (MQ=255)                        ggCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCca                                                                                                                <  2:691980/125‑1 (MQ=255)                        ggCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTAt                                                                                                  >  1:253871/1‑139 (MQ=255)                        ggCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTAt                                                                                                  >  2:327566/1‑139 (MQ=255)                        ggCGTTCGTCAATGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTAt                                                                                                  >  2:405831/1‑139 (MQ=255)                                tCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCtgctgc                                                                                          <  2:253871/139‑1 (MQ=255)                                        gTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCaa                                                                                  <  2:225893/139‑1 (MQ=255)                                        gTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCaa                                                                                  >  2:157145/1‑139 (MQ=255)                                                 ttgtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGagaaga                                                                         <  1:168312/139‑1 (MQ=255)                                                              aaGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATc                                                            >  1:233000/1‑139 (MQ=255)                                                              aaGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATc                                                            >  2:272693/1‑139 (MQ=255)                                                              aaGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATc                                                            >  2:748727/1‑139 (MQ=255)                                                                     gCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTg                                                     >  2:528799/1‑139 (MQ=255)                                                                       tGCGCCACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTGCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGtt                                                   <  2:569062/139‑1 (MQ=255)                                                                        gcgcAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTc                                                  <  2:715526/139‑1 (MQ=255)                                                                             aCGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGaa                                             <  2:626888/139‑1 (MQ=255)                                                                                  cAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGa                                        <  1:272693/139‑1 (MQ=255)                                                                                          tGGCGTGCTGGTCTCGCTTCGACTTCACCGTCACTTCACATAACTTCATATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGGAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTg                                >  1:597259/1‑139 (MQ=255)                                                                                          tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAg                                                                                                           >  1:320100/1‑64 (MQ=255)                                                                                          tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAg                                                                                                           <  2:320100/64‑1 (MQ=255)                                                                                          tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCTCTGTTCTGTAAAATGACTAGCCTg                                >  2:449208/1‑139 (MQ=255)                                                                                          tGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTg                                >  1:302583/1‑139 (MQ=255)                                                                                            gCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGtt                              <  2:791361/139‑1 (MQ=255)                                                                                               tGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTgg                           >  2:455915/1‑139 (MQ=255)                                                                                               tGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTgg                           <  2:302583/139‑1 (MQ=255)                                                                                                 cTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTg                         <  1:748727/139‑1 (MQ=255)                                                                                                 cTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTg                         <  1:449208/139‑1 (MQ=255)                                                                                                 cTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTg                         <  1:364179/139‑1 (MQ=255)                                                                                                 cTGGCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTg                         <  1:157145/139‑1 (MQ=255)                                                                                                    gCCTCGCTTCGACTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAg                      >  1:123704/1‑139 (MQ=255)                                                                                                               aCTTCACCGTCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtgttg           >  2:562317/1‑139 (MQ=255)                                                                                                                        tCACTTCACATAACTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCg  <  2:233000/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                               CAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCG  >  NC_000913/4637989‑4638246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑