Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	391,739	C→T	*289* (TAG→TAA)	insF1 ←	IS3 transposase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	391,739	0	C	T	100.0%	74.7 / NA	26	*289* (TAG→TAA)	insF1	IS3 transposase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/14);  total (12/14)

TCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCC  >  NC_000913/391604‑391844                                                                                                                                        |                                                                                                            tcttcGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTaa                                                                                                          >  2:588970/1‑139 (MQ=255)            cATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCtt                                                                                               >  2:109978/1‑139 (MQ=255)                                       cGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgcc                                                                    <  2:331083/139‑1 (MQ=255)                                       cGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgcc                                                                    <  1:273140/139‑1 (MQ=255)                                         atcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgc                                                                  >  2:25797/1‑139 (MQ=255)                                         atcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgc                                                                  >  2:468090/1‑139 (MQ=255)                                          tcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgc‑‑c                                                               >  2:447654/1‑139 (MQ=255)                                             tcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑caca                                                            <  1:109978/139‑1 (MQ=255)                                              cTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑Cacac                                                           >  1:532290/1‑139 (MQ=255)                                                           ccTACAGTACTGTAGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCaaa                                                                                      <  2:210217/101‑1 (MQ=255)                                                           ccTACAGTACTGTAGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCaaa                                                                                      >  1:210217/1‑101 (MQ=255)                                                            cTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTgccgccg                                             <  2:114252/139‑1 (MQ=255)                                                             tACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTgccgccgc                                            >  2:592611/1‑139 (MQ=255)                                                                    cTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGAt                                     >  2:429969/1‑139 (MQ=255)                                                                       ttGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGt                                  >  1:480267/1‑139 (MQ=255)                                                                       ttGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGt                                  <  1:80193/139‑1 (MQ=255)                                                                                 cGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTc                        >  2:165517/1‑139 (MQ=255)                                                                                   aaTCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGa                      <  1:462292/139‑1 (MQ=255)                                                                                     tCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGata                    <  1:592611/139‑1 (MQ=255)                                                                                     tCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGata                    <  2:351878/139‑1 (MQ=255)                                                                                             gtgggGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTGCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAACTaaa            <  1:468090/139‑1 (MQ=255)                                                                                                gggggCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGa                      >  1:113091/1‑126 (MQ=37)                                                                                                gggggCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGa                      <  2:113091/126‑1 (MQ=37)                                                                                                  gggCTGATTGATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCg       <  2:729622/139‑1 (MQ=18)                                                                                                      tgattgatCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGtt     <  1:25797/139‑1 (MQ=255)                                                                                                       gattgatCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGcc  <  1:672144/139‑1 (MQ=14)                                                                                                                                        |                                                                                                            TCTTCGCTGAACATTGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGC‑‑CACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCC  >  NC_000913/391604‑391844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑