| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 411,829 | C→T | W218* (TGG→TAG) | araJ ← | L‑arabinose‑inducible putative transporter, MFS family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 411,829 | 0 | C | T | 92.3% | 65.7 / ‑1.0 | 26 | W218* (TGG→TAG) | araJ | L‑arabinose‑inducible putative transporter, MFS family |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (16/8); total (17/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
AATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGAC > NC_000913/411695‑411962 | aaTAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGc > 1:353887/1‑139 (MQ=255) cGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAaca > 1:122105/1‑139 (MQ=255) tCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAacaca > 2:376251/1‑139 (MQ=255) tCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAacaaa > 1:642733/1‑137 (MQ=255) aaTCCTGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAAAAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGTAAACACACCTGCg < 2:25880/139‑1 (MQ=255) tGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTg > 1:190731/1‑132 (MQ=255) tGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTg < 2:190731/132‑1 (MQ=255) gCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCa > 1:167666/1‑139 (MQ=255) tatTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAGACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTg < 1:726743/120‑1 (MQ=255) tatTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAGACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTg > 2:726743/1‑120 (MQ=255) ttCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTggcggc < 1:376251/139‑1 (MQ=255) ttCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGCCAAACACACCTGCGTTGCCAAACAGCGTggcggc > 2:515582/1‑139 (MQ=255) cACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAAtt < 2:122105/139‑1 (MQ=255) gcccTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCa > 1:502059/2‑139 (MQ=255) aCTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGc < 2:642733/139‑1 (MQ=255) aCTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGc > 2:477780/1‑139 (MQ=255) aTCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGtt > 1:165876/1‑139 (MQ=255) cGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCgcgc < 1:282641/139‑1 (MQ=255) ttCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGa < 2:165876/139‑1 (MQ=255) tCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCCAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTCAAATTGTTCGCGCAGAt < 2:502059/139‑1 (MQ=255) catGTATGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAata > 2:31115/1‑139 (MQ=255) aTGGCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTgg > 2:107530/1‑139 (MQ=255) ggCTTTACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCa > 1:141465/1‑139 (MQ=255) tttACGTAGCTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCAccc > 2:728474/1‑139 (MQ=255) cTGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGa > 1:585343/1‑139 (MQ=255) tGAACTAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGAc > 2:415958/1‑139 (MQ=255) | AATAACGTCCTGAAATCCTGCCACTTAGCATATTTCCCAGCACCATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGAC > NC_000913/411695‑411962 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |