Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	47,776	G→A	*177* (TAG→TAA)  S3N (AGC→AAC)	kefF → kefC →	potassium‑efflux system ancillary protein for KefC, glutathione‑regulated, quinone oxidoreductase, FMN‑dependent potassium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	47,776	0	G	A	95.0%	52.5 / ‑6.2	20	*177* (TAG→TAA)  S3N (AGC→AAC)	kefF kefC	potassium‑efflux system ancillary protein for KefC, glutathione‑regulated, quinone oxidoreductase, FMN‑dependent potassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (10/9);  minor base T (0/1);  total (10/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGC  >  NC_000913/47652‑47907                                                                                                                             |                                                                                                                                    ggCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTc                                                                                                                       >  1:101410/1‑139 (MQ=255)       aCTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCTCATCATGGATATCCATACGCTGATTCAGgcgc                                                                                                                 <  1:11367/139‑1 (MQ=255)                     tttGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGt                                                                                                   >  2:28417/1‑139 (MQ=255)                                   cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCgg                                                                                               <  1:488315/129‑1 (MQ=255)                                   cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCgg                                                                                               >  2:488315/1‑129 (MQ=255)                                   cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAt                                                                                     >  2:694515/1‑139 (MQ=255)                                      cTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGt                                                                                  <  1:52570/139‑1 (MQ=255)                                                        tcccccCGAAGGGCCGGCGGGTCGCTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCGGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt                                                                <  1:592233/137‑1 (MQ=255)                                                        aaCCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt                                                                <  1:28417/139‑1 (MQ=255)                                                                   ggCAGGCGGGTCACTATAAGCAACGTCTGCTGGAATGGCATGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGAt                                                     <  2:683812/139‑1 (MQ=255)                                                                                   taAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg                                                                                                                  >  2:706631/1‑62 (MQ=255)                                                                                   taAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg                                                                                                                  <  1:706631/62‑1 (MQ=255)                                                                                          cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg                                                                       <  1:678767/98‑1 (MQ=255)                                                                                          cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg                                                                       >  2:678767/1‑98 (MQ=255)                                                                                           gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGAt                                                     >  2:429727/1‑115 (MQ=255)                                                                                           gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGAt                                                     <  1:429727/115‑1 (MQ=255)                                                                                           gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg                             >  2:637959/1‑139 (MQ=255)                                                                                               gctgGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTg                         >  2:335245/1‑139 (MQ=255)                                                                                                             aggCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGt           >  1:344364/1‑139 (MQ=255)                                                                                                                      atGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGc  <  1:67809/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                    GGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGC  >  NC_000913/47652‑47907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑