Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 634,746 2 bp→AT [ybdL][ybdM] [ybdL], [ybdM]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913634,7460GA100.0% 46.5 / NA 16*387* (TAG→TAAybdLmethionine aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)
*NC_000913634,7470CT100.0% 47.2 / NA 16*210* (TAG→TAAybdMSpo0J family protein, ParB‑like nuclease domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

AGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTA  >  NC_000913/634616‑634867
                                                                                                                                  ||                                                                                                                        
aGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTaa                                                                                                                   <  2:322317/139‑1 (MQ=255)
       cGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCa                                                                                                            >  2:411928/1‑139 (MQ=255)
           gcggcgATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGAAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGgcg                                                                                                        <  1:526846/139‑1 (MQ=255)
                                                      aTAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTaa                                                             >  1:203902/1‑139 (MQ=255)
                                                              aTTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTc                                                     >  2:91688/1‑139 (MQ=255)
                                                                tCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAg                                                   >  1:522257/1‑139 (MQ=255)
                                                                    ctctGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGgcgc                                                                                                       <  1:59999/83‑1 (MQ=255)
                                                                    ctctGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGgcgc                                                                                                       >  2:59999/1‑83 (MQ=255)
                                                                        gTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCgcagca                                           >  1:212115/1‑139 (MQ=255)
                                                                         ttttGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCAc                                          >  2:3304/1‑139 (MQ=255)
                                                                                 agaagGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGc                                  <  2:101335/139‑1 (MQ=255)
                                                                                   aagGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCg                                                                                      <  1:224447/85‑1 (MQ=255)
                                                                                   aagGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCg                                                                                      >  2:224447/1‑85 (MQ=255)
                                                                                    agGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGt                               <  1:644233/139‑1 (MQ=255)
                                                                                                            cTGAACGCCTGCGCCAGCTTTAATTATTTACCCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAAttt       <  1:188135/139‑1 (MQ=255)
                                                                                                                 cGCCTGCGCCAACTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCACTTttatta  <  2:522257/139‑1 (MQ=255)
                                                                                                                                  ||                                                                                                                        
AGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTA  >  NC_000913/634616‑634867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: