Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 582,148 Δ1 bp intergenic (+51/+4) tfaD → / ← ybcY pseudogene, DLP12 prophage, tail fiber assembly protein family,Phage or Prophage Related/pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913582,1470G.99.1% 418.8 / ‑1.8 106intergenic (+50/+5)tfaD/ybcYpseudogene, DLP12 prophage, tail fiber assembly protein family,Phage or Prophage Related/pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (51/53);  minor base T (0/1);  total (52/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 111 total aligned reads displayed.

GGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTT  >  NC_000913/582012‑582280
                                                                                                                                       |                                                                                                                                     
ggAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTgatg                                                                                                                                    >  1:1120896/1‑136 (MQ=255)
 gAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTgatgt                                                                                                                                   >  2:154871/1‑135 (MQ=255)
 gAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTgatgt                                                                                                                                   >  2:587533/1‑135 (MQ=255)
                    cTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCgg                                                                                                               >  2:365416/1‑139 (MQ=255)
                      gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                             <  1:157768/139‑1 (MQ=255)
                      gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaa                                                                                                             <  1:1165640/139‑1 (MQ=255)
                           gtgtTGATTCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                        <  1:1091600/139‑1 (MQ=255)
                                       cAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAcc                                                                                            >  2:924160/1‑139 (MQ=255)
                                        aaCTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGGTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCt                                                                                           >  1:1421081/1‑139 (MQ=255)
                                         aCTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCtt                                                                                          >  2:1596900/1‑139 (MQ=255)
                                         aCTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCtt                                                                                          >  1:1683687/1‑139 (MQ=255)
                                              aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                     <  1:587533/139‑1 (MQ=255)
                                              aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                     <  2:1683687/139‑1 (MQ=255)
                                               ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTgatgt                                                                                                                                   <  2:938883/93‑5 (MQ=255)
                                               ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTgatgt                                                                                                                                   >  1:938883/1‑89 (MQ=255)
                                               ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                    >  1:1680500/1‑139 (MQ=255)
                                               ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                    >  2:396036/1‑139 (MQ=255)
                                               ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                    <  1:1400900/139‑1 (MQ=255)
                                                     atTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGttt                                                                              <  1:860720/139‑1 (MQ=255)
                                                       tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                            >  1:106440/1‑139 (MQ=255)
                                                        gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                           <  1:987539/139‑1 (MQ=255)
                                                         aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                           <  2:336047/106‑1 (MQ=255)
                                                         aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGaaaa                                                                                                           >  1:336047/1‑106 (MQ=255)
                                                             gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTg                                                                                                                     <  1:914878/92‑1 (MQ=255)
                                                             gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTg                                                                                                                     >  2:914878/1‑92 (MQ=255)
                                                             gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                    >  1:1445179/1‑125 (MQ=255)
                                                             gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                    <  2:1445179/125‑1 (MQ=255)
                                                             gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                      >  2:21024/1‑139 (MQ=255)
                                                              ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCg                                                                                                                <  1:1142971/96‑1 (MQ=255)
                                                              ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCg                                                                                                                >  2:1142971/1‑96 (MQ=255)
                                                              ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                    <  1:1225283/124‑1 (MQ=255)
                                                              ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                    >  2:1225283/1‑124 (MQ=255)
                                                                tACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                        <  2:622719/102‑1 (MQ=255)
                                                                tACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                        >  1:622719/1‑102 (MQ=255)
                                                                 aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                  <  1:1365386/139‑1 (MQ=255)
                                                                  cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                 <  1:1596900/139‑1 (MQ=255)
                                                                   gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                <  2:1261455/139‑1 (MQ=255)
                                                                        cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                    >  2:1537715/1‑114 (MQ=255)
                                                                        cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                    <  1:1537715/114‑1 (MQ=255)
                                                                        cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                >  1:722951/1‑134 (MQ=255)
                                                                        cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                           >  2:777127/1‑139 (MQ=255)
                                                                        cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                           <  1:365416/139‑1 (MQ=255)
                                                                         tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                          <  2:106440/139‑1 (MQ=255)
                                                                         tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                          <  1:777127/139‑1 (MQ=255)
                                                                          gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTg                                                         >  1:21049/1‑139 (MQ=255)
                                                                           tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTg                                                                                                                     >  1:831497/1‑78 (MQ=255)
                                                                           tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTg                                                                                                                     <  2:831497/78‑1 (MQ=255)
                                                                           tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                        <  1:976530/139‑1 (MQ=255)
                                                                            cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                 >  1:1692161/1‑97 (MQ=255)
                                                                               ggAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGt                                                    >  1:495522/1‑139 (MQ=255)
                                                                                 aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                  >  1:41244/1‑139 (MQ=255)
                                                                                  gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                 <  2:1251607/139‑1 (MQ=255)
                                                                                  gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                 <  2:1680500/139‑1 (MQ=255)
                                                                                     aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTccag                                                                                                                     <  1:1078768/68‑3 (MQ=255)
                                                                                     aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTg                                                                                                                     >  2:1078768/1‑68 (MQ=255)
                                                                                     aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                  >  1:1698816/1‑119 (MQ=255)
                                                                                     aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                  <  2:1698816/119‑1 (MQ=255)
                                                                                          tGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATTCTCTGAGAGTATATGTGTTaa                                         >  2:1705723/1‑139 (MQ=255)
                                                                                           gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAg                                        >  1:1761004/1‑139 (MQ=255)
                                                                                             gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGa                                                                                                              <  2:32862/67‑1 (MQ=255)
                                                                                             gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGa                                                                                                              >  1:32862/1‑67 (MQ=255)
                                                                                                tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttc                                   >  1:924302/1‑139 (MQ=255)
                                                                                                tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTAATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttc                                   >  1:1268470/1‑139 (MQ=255)
                                                                                                 aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCg                                                                                                                <  2:1126887/61‑1 (MQ=255)
                                                                                                 aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCg                                                                                                                >  1:1126887/1‑61 (MQ=255)
                                                                                                 aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                      <  2:188179/71‑1 (MQ=255)
                                                                                                       cagcaCGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                           >  2:963668/1‑92 (MQ=255)
                                                                                                       cagcaCGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                           <  1:963668/92‑1 (MQ=255)
                                                                                                       cagcaCGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                        >  1:205281/1‑111 (MQ=255)
                                                                                                       cagcaCGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                        <  2:205281/111‑1 (MQ=255)
                                                                                                            cGTCTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt                       >  1:982319/1‑139 (MQ=255)
                                                                                                               cTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTtgt                    <  2:1268470/139‑1 (MQ=255)
                                                                                                               cTTAAGCAAGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTtgt                    <  2:924302/139‑1 (MQ=255)
                                                                                                                       aGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                <  1:185716/87‑1 (MQ=255)
                                                                                                                       aGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                >  2:185716/1‑87 (MQ=255)
                                                                                                                       aGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                            >  1:744249/1‑91 (MQ=255)
                                                                                                                       aGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                            <  2:744249/91‑1 (MQ=255)
                                                                                                                       aGAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa            >  2:556670/1‑139 (MQ=255)
                                                                                                                        gAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGt                                                                                               <  1:1173091/55‑1 (MQ=255)
                                                                                                                        gAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGt                                                                                               >  2:1173091/1‑55 (MQ=255)
                                                                                                                        gAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                          <  2:150102/92‑1 (MQ=255)
                                                                                                                        gAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                          <  1:159205/92‑1 (MQ=255)
                                                                                                                        gAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                          >  1:150102/1‑92 (MQ=255)
                                                                                                                        gAACATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                          >  2:159205/1‑92 (MQ=255)
                                                                                                                         aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAg                                                                                                                  <  1:3137/35‑1 (MQ=25)
                                                                                                                         aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                          >  1:1151091/1‑91 (MQ=255)
                                                                                                                         aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                          <  2:1151091/91‑1 (MQ=255)
                                                                                                                         aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg          >  2:364197/1‑139 (MQ=255)
                                                                                                                         aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg          >  2:124275/1‑139 (MQ=255)
                                                                                                                         aaCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg          <  2:1445674/139‑1 (MQ=255)
                                                                                                                         aaCATGCTGCGGTCGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg          >  2:1314944/1‑139 (MQ=255)
                                                                                                                          aCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCt                                                                                           >  1:864510/1‑57 (MQ=255)
                                                                                                                          aCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCt                                                                                           <  2:864510/57‑1 (MQ=255)
                                                                                                                          aCATGCTGCGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGa         <  2:21049/139‑1 (MQ=255)
                                                                                                                             tgctgcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGc      >  2:362931/1‑139 (MQ=255)
                                                                                                                             tgctgcGTTTGATGTTATTTTTTTCCTAAAGCGGAAAACATTACTACAGTACCTTGAACCTTAGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGc      <  1:1314944/139‑1 (MQ=255)
                                                                                                                              gctgcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATg                                                              <  1:1366047/82‑1 (MQ=255)
                                                                                                                                 gcGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt  <  2:1761004/139‑1 (MQ=255)
                                                                                                                                  cGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                >  1:282481/1‑76 (MQ=255)
                                                                                                                                  cGGTTGATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                <  2:282481/76‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTT  >  NC_000913/582012‑582280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: