Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,139,406 Δ1 bp coding (1029/1644 nt) flgK → flagellar hook‑filament junction protein 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,139,4060T.100.0% 303.5 / NA 74coding (1029/1644 nt)flgKflagellar hook‑filament junction protein 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (36/38);  total (36/38)

GGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGA  >  NC_000913/1139272‑1139530
                                                                                                                                      |                                                                                                                            
ggACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACAAAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGt                                                                                                                         >  1:1273652/1‑139 (MQ=255)
 gACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGtt                                                                                                                        >  2:1634893/1‑139 (MQ=255)
 gACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGtt                                                                                                                        >  1:277668/1‑139 (MQ=255)
      cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGAt                                                                                                                   <  2:1294546/139‑1 (MQ=255)
          cgcTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGt                                                                                                               <  1:1240839/139‑1 (MQ=255)
            cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGc                                                                                                             <  2:1681751/139‑1 (MQ=255)
            cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGc                                                                                                             <  1:180315/139‑1 (MQ=255)
            cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGc                                                                                                             <  1:240363/139‑1 (MQ=255)
                cATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGAt                                                                                                                   >  1:346677/1‑129 (MQ=255)
                cATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGAt                                                                                                                   <  2:346677/129‑1 (MQ=255)
                 aTTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACgg                                                                                                        <  1:114665/139‑1 (MQ=255)
                         aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                >  2:222804/1‑139 (MQ=255)
                         aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                >  1:735396/1‑139 (MQ=255)
                          ggCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                              <  1:1720720/139‑1 (MQ=255)
                              ttCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                              >  1:118371/1‑136 (MQ=255)
                              ttCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                              <  2:118371/136‑1 (MQ=255)
                              ttCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTc                                                                                           >  1:408595/1‑139 (MQ=255)
                               tCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTcc                                                                                          >  2:1327954/1‑139 (MQ=255)
                                cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                         <  1:1634893/139‑1 (MQ=255)
                                cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                         >  2:1393002/1‑139 (MQ=255)
                                  acacCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcgcg                                                                                       <  1:516775/139‑1 (MQ=255)
                                     cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                    >  2:1055045/1‑139 (MQ=255)
                                     cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                    >  2:491262/1‑139 (MQ=255)
                                     cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCGGCAAAACAAGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCGCCGCGGtg                                                                                    <  2:1273652/139‑2 (MQ=255)
                                      ccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTAc                                                                                   >  1:884311/1‑139 (MQ=255)
                                        aaCACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTg                                                                                 >  2:270014/1‑139 (MQ=255)
                                           acaAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCg                                                                              >  1:966693/1‑139 (MQ=255)
                                            caAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGa                                                                             <  1:491262/139‑1 (MQ=255)
                                             aaaGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGAc                                                                            <  1:222804/139‑1 (MQ=255)
                                               aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACa                                                                           >  1:53176/1‑138 (MQ=255)
                                               aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACa                                                                           <  2:53176/138‑1 (MQ=255)
                                               aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAg                                                                          >  2:1180362/1‑139 (MQ=255)
                                               aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAg                                                                          >  1:1754108/1‑139 (MQ=255)
                                               aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGCTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAg                                                                          >  2:1241672/1‑139 (MQ=255)
                                               aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGAt                                                                                                                   <  1:67541/97‑1 (MQ=255)
                                               aGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGAt                                                                                                                   >  2:67541/1‑97 (MQ=255)
                                                    gATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTAc                                                                     >  2:1600215/1‑139 (MQ=255)
                                                             cTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCg                                                            <  1:750894/139‑1 (MQ=255)
                                                               aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          <  2:735396/139‑1 (MQ=255)
                                                               aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          <  1:1787577/139‑1 (MQ=255)
                                                               aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          >  2:1787577/1‑139 (MQ=255)
                                                               aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          <  2:1796309/139‑1 (MQ=255)
                                                               aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          <  1:1393002/139‑1 (MQ=255)
                                                                aCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTc                                                         >  1:1637331/1‑139 (MQ=255)
                                                                     gATGCCGGTGACGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataa                                                    >  1:224859/1‑139 (MQ=255)
                                                                     gATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataa                                                    >  1:404927/1‑139 (MQ=255)
                                                                     gATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGacaa                                                    <  2:966693/139‑1 (MQ=255)
                                                                       tGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataata                                                  >  1:1600758/1‑139 (MQ=255)
                                                                         ccGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATaataat                                                <  2:224859/139‑1 (MQ=255)
                                                                            gTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAg                                             >  1:1296123/1‑139 (MQ=255)
                                                                                aGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGc                                         <  1:872178/139‑1 (MQ=255)
                                                                                  aTTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAg                                       <  1:1180362/139‑1 (MQ=255)
                                                                                  aTTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAg                                       <  1:1241672/139‑1 (MQ=255)
                                                                                         tGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                         <  2:1369960/82‑1 (MQ=255)
                                                                                         tGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                         >  1:1369960/1‑82 (MQ=255)
                                                                                         tGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTgg                                          >  1:1751872/1‑129 (MQ=255)
                                                                                         tGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTgg                                          <  2:1751872/129‑1 (MQ=255)
                                                                                            tATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGcc                             <  2:1754108/139‑1 (MQ=255)
                                                                                              tCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCtt                           <  2:1600758/139‑1 (MQ=255)
                                                                                                 gTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATaataat                                                >  1:110330/1‑115 (MQ=255)
                                                                                                 gTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATaataat                                                <  2:110330/115‑1 (MQ=255)
                                                                                                 gTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGcc                        <  2:1296123/139‑1 (MQ=255)
                                                                                                   aaGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAg                      <  2:408595/139‑1 (MQ=255)
                                                                                                        cgcgGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          >  1:1514713/1‑98 (MQ=255)
                                                                                                        cgcgGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                          <  2:1514713/98‑1 (MQ=255)
                                                                                                         gcgGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                <  2:277668/139‑1 (MQ=255)
                                                                                                          cgGTTCTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCtt                           >  2:1167117/1‑127 (MQ=255)
                                                                                                          cgGTTCTGCAAAACACGAAAAACAAAGGGACATTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCtt                           <  1:1167117/127‑1 (MQ=255)
                                                                                                               cTGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACttt          >  1:1217748/1‑139 (MQ=255)
                                                                                                                tGCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACtttt         >  1:406250/1‑139 (MQ=255)
                                                                                                                 gCAAAACACGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTa        <  1:1600215/139‑1 (MQ=255)
                                                                                                                       cacGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAg                                                                          <  1:1590372/67‑1 (MQ=255)
                                                                                                                       cacGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAg                                                                          >  2:1590372/1‑67 (MQ=255)
                                                                                                                       cacGAAAAACAAAGGGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa  >  1:998314/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                            
GGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGA  >  NC_000913/1139272‑1139530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: