Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,5370C.100.0% 193.8 / NA 48coding (1052/1404 nt)fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (22/26);  total (22/26)

CTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAT  >  NC_000913/1685401‑1685664
                                                                                                                                        |                                                                                                                               
cTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGccc                                                                                                                               <  1:541890/139‑1 (MQ=255)
 tCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGcccc                                                                                                                              >  2:542526/1‑139 (MQ=255)
               aaTACCCATTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGCTTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCa                                                                                                               <  1:1090723/139‑1 (MQ=255)
                aTACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAc                                                                                                              <  2:1354542/139‑1 (MQ=255)
                      aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTCCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAACGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGtt                                                                                                        >  2:624269/1‑139 (MQ=255)
                        tGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAcgtcgt                                                                                                         >  1:478057/1‑136 (MQ=255)
                        tGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCAcgtcgt                                                                                                         <  2:478057/136‑1 (MQ=255)
                        tGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTcc                                                                                                      >  1:662006/1‑139 (MQ=255)
                         gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                     >  1:1515829/1‑139 (MQ=255)
                          cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTcccc                                                                                                    <  2:1389387/139‑1 (MQ=255)
                                    gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                     >  2:120088/1‑128 (MQ=255)
                                    gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                     <  1:120088/128‑1 (MQ=255)
                                      tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCGTGTTGATCGCTACGTCGTACCCCATCACCTGAcag                                                                                        <  1:1317349/139‑1 (MQ=255)
                                      tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                        <  1:974319/139‑1 (MQ=255)
                                         aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCt                                                                                             >  1:1463840/1‑131 (MQ=255)
                                         aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCt                                                                                             <  2:1463840/131‑1 (MQ=255)
                                           cTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAACGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                  >  1:446981/1‑124 (MQ=255)
                                           cTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                  <  2:446981/124‑1 (MQ=255)
                                           cTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                >  2:1081836/1‑126 (MQ=255)
                                           cTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                <  1:1081836/126‑1 (MQ=255)
                                           cTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCagag                                                                                   <  2:536066/139‑1 (MQ=255)
                                              tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCAt                                                                                >  2:436416/1‑139 (MQ=255)
                                               ccATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGcc                                                                                                                                <  1:296045/91‑1 (MQ=255)
                                               ccATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGcc                                                                                                                                >  2:296045/1‑91 (MQ=255)
                                                                   cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                           >  2:1039812/1‑139 (MQ=255)
                                                                   cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                           >  1:1785988/1‑139 (MQ=255)
                                                                      aCCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                                                        <  1:1039812/139‑1 (MQ=255)
                                                                                  aaaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGcc                                                                                                                                <  1:1597207/56‑1 (MQ=255)
                                                                                  aaaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGcc                                                                                                                                >  2:1597207/1‑56 (MQ=255)
                                                                                  aaaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                            <  2:1156735/139‑1 (MQ=255)
                                                                                             cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTg                                                         <  1:1697676/115‑1 (MQ=255)
                                                                                             cACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTg                                                         >  2:1697676/1‑115 (MQ=255)
                                                                                              aCCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                            >  1:252288/1‑127 (MQ=255)
                                                                                              aCCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                            <  2:252288/127‑1 (MQ=255)
                                                                                               ccATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGCTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAg                               <  2:1409775/139‑1 (MQ=255)
                                                                                                 aTTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCt                             >  2:1018951/1‑139 (MQ=255)
                                                                                                    ggACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGcc                          >  2:1706947/1‑139 (MQ=255)
                                                                                                       cGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCt                                                                                             >  2:1660202/1‑69 (MQ=255)
                                                                                                       cGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCt                                                                                             <  1:1660202/69‑1 (MQ=255)
                                                                                                       cGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCgg                       >  1:275493/1‑139 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                     <  1:304449/58‑1 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTccc                                                                                                     >  2:304449/1‑58 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                        >  2:729965/1‑71 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                        <  1:729965/71‑1 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTc                                              <  1:458535/113‑1 (MQ=255)
                                                                                                          aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTc                                              >  2:458535/1‑113 (MQ=255)
                                                                                                                ttcagttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTc              <  2:1515829/139‑1 (MQ=255)
                                                                                                                  cagttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCg            <  1:436416/139‑1 (MQ=255)
                                                                                                                    gttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                 >  2:328567/1‑68 (MQ=255)
                                                                                                                    gttcaAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                 <  1:328567/68‑1 (MQ=255)
                                                                                                                       caAAGTTACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGAtt       <  1:542526/139‑1 (MQ=255)
                                                                                                                            ttACCGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAt  <  2:275493/139‑1 (MQ=255)
                                                                                                                               ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                           <  2:1450189/63‑1 (MQ=255)
                                                                                                                               ccGGAAGCGCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                           >  1:1450189/1‑63 (MQ=255)
                                                                                                                                        |                                                                                                                               
CTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAT  >  NC_000913/1685401‑1685664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: