Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,8810C.96.1% 298.2 / 4.3 77intergenic (+235/‑131)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base . (37/37);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAA  >  NC_000913/2462744‑2463008
                                                                                                                                         |                                                                                                                               
tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcc                                                                                                                                <  2:975074/139‑1 (MQ=255)
 gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccc                                                                                                                               <  1:430288/139‑1 (MQ=255)
  cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                                                                                                              <  2:20313/139‑1 (MQ=255)
   aaaCAGCCTGTTGGCAGCGTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccccc                                                                                                                             <  2:1210008/139‑1 (MQ=255)
     aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                            <  1:817629/138‑1 (MQ=255)
     aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                            >  2:817629/1‑138 (MQ=255)
                 cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              >  2:1340938/1‑139 (MQ=255)
                 cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              <  2:620565/139‑1 (MQ=255)
                   gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                            >  1:680284/1‑139 (MQ=255)
                   gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                            <  2:603004/139‑1 (MQ=255)
                   gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                            >  1:313277/1‑139 (MQ=255)
                     ttGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCt                                                                                                          >  1:740901/1‑139 (MQ=255)
                       gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCa                                                                                                        <  1:1340938/139‑1 (MQ=255)
                       gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCa                                                                                                        >  2:1270424/1‑139 (MQ=255)
                           tttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATaca                                                                                                    <  2:313277/139‑1 (MQ=255)
                             tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCTTTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                                                  <  1:1270424/139‑1 (MQ=255)
                             tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                                                  <  2:523071/139‑1 (MQ=255)
                                      tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                                                                                                              >  2:1339661/1‑103 (MQ=255)
                                      tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                                                                                                              <  1:1339661/103‑1 (MQ=255)
                                      tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTaaa                                                                                         >  1:843312/1‑139 (MQ=255)
                                         gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      <  2:1755205/139‑1 (MQ=255)
                                              aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcc                                                                                                                                >  2:1541943/1‑93 (MQ=255)
                                              aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcc                                                                                                                                <  1:1541943/93‑1 (MQ=255)
                                                    aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                                                  >  1:163877/1‑117 (MQ=255)
                                                    aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                                                  <  2:163877/117‑1 (MQ=255)
                                                    aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              <  1:1749719/104‑1 (MQ=255)
                                                    aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              >  2:1749719/1‑104 (MQ=255)
                                                    aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                                                           <  1:216735/123‑1 (MQ=255)
                                                    aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                                                           >  2:216735/1‑123 (MQ=255)
                                                    aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      >  2:1196458/1‑128 (MQ=255)
                                                    aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      <  1:1196458/128‑1 (MQ=255)
                                                         aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              <  2:1073702/99‑1 (MQ=255)
                                                         aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              >  1:1073702/1‑99 (MQ=255)
                                                          aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGa                                                                     >  2:147469/1‑139 (MQ=255)
                                                           ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                    <  1:597618/139‑1 (MQ=255)
                                                           ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                    >  1:1514814/1‑139 (MQ=255)
                                                           ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                    <  2:740901/139‑1 (MQ=255)
                                                              gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGa                                                                                       <  1:546208/117‑1 (MQ=255)
                                                              gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGa                                                                                       >  2:546208/1‑117 (MQ=255)
                                                                aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                            <  2:545244/94‑1 (MQ=255)
                                                                aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                            >  1:545244/1‑94 (MQ=255)
                                                                     aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                          <  2:1514814/139‑1 (MQ=255)
                                                                      aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGGGTTTTTTgtt                                                         >  1:358050/1‑137 (MQ=255)
                                                                      aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                                                         >  2:28239/1‑139 (MQ=255)
                                                                      aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                                                         >  1:471925/1‑139 (MQ=255)
                                                                         cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                      <  1:115477/139‑1 (MQ=255)
                                                                           tatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              >  1:718257/1‑81 (MQ=255)
                                                                           tatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              <  2:718257/81‑1 (MQ=255)
                                                                            atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                            <  1:863399/67‑1 (MQ=255)
                                                                            atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                            >  2:863399/1‑67 (MQ=255)
                                                                            atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                   >  1:1593602/1‑139 (MQ=255)
                                                                                  aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtg                                                                                                                >  2:549667/1‑72 (MQ=255)
                                                                                  aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtg                                                                                                                <  1:549667/72‑1 (MQ=255)
                                                                                  aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                             <  1:147469/139‑1 (MQ=255)
                                                                                   ggCGTTCTGCTCCTTCTTCTCAAAAAGTTTACTTTTCTGCATTTCCCGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACCCTCAAATTAAAGATAGGTTCCAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                            <  2:358050/139‑1 (MQ=255)
                                                                                    gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctgg                                                                                                                       <  2:4149/64‑4 (MQ=255)
                                                                                    gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctgg                                                                                                                       >  1:4149/1‑61 (MQ=255)
                                                                                      gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                         <  2:843312/139‑1 (MQ=255)
                                                                                       ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                    >  2:884245/1‑111 (MQ=255)
                                                                                       ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                    <  1:884245/111‑1 (MQ=255)
                                                                                       ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg                                          >  2:857539/1‑137 (MQ=255)
                                                                                       ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg                                          <  1:857539/137‑1 (MQ=255)
                                                                                        tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTTCATTTCCAGGATACCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                       <  1:514110/139‑1 (MQ=255)
                                                                                        tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              >  2:521627/1‑68 (MQ=255)
                                                                                        tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATtgtg                                                                                                              <  1:521627/68‑1 (MQ=255)
                                                                                         cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                                                                                                              <  1:1741877/52‑1 (MQ=255)
                                                                                         cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccc                                                                                                                              >  2:1741877/1‑52 (MQ=255)
                                                                                            cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      >  1:423169/1‑88 (MQ=255)
                                                                                            cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      <  2:423169/88‑1 (MQ=255)
                                                                                                    tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                            >  2:246792/1‑58 (MQ=255)
                                                                                                    tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTgcg                                                                                                            <  1:246792/58‑1 (MQ=255)
                                                                                                      tcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      <  1:1295767/78‑1 (MQ=255)
                                                                                                      taaaaGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      >  2:1295767/3‑78 (MQ=255)
                                                                                                         aaGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCa                                                                                                        <  2:770871/57‑1 (MQ=255)
                                                                                                         aaGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCa                                                                                                        >  1:770871/1‑57 (MQ=255)
                                                                                                              tttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGGGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                      <  2:836065/102‑1 (MQ=255)
                                                                                                              tttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGGGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                      >  1:836065/1‑102 (MQ=255)
                                                                                                              tttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                                                  <  1:1124322/58‑1 (MQ=255)
                                                                                                              tttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      <  2:187765/70‑1 (MQ=255)
                                                                                                              tttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                      >  1:187765/1‑70 (MQ=255)
                                                                                                              tttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                       >  2:425155/1‑117 (MQ=255)
                                                                                                              tttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                       <  1:425155/117‑1 (MQ=255)
                                                                                                              tttACTTTTCTGCATTTACAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                                                  >  2:1124322/1‑58 (MQ=255)
                                                                                                               ttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                   <  2:1619747/72‑1 (MQ=255)
                                                                                                               ttACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                   >  1:1619747/1‑72 (MQ=255)
                                                                                                                tACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATacac                                                                                                   <  2:1638449/55‑1 (MQ=255)
                                                                                                                tACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATacac                                                                                                   >  1:1638449/1‑55 (MQ=255)
                                                                                                                tACTTTTCTGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaatc               >  2:581495/1‑139 (MQ=255)
                                                                                                                        tGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                   >  2:1502820/1‑95 (MQ=255)
                                                                                                                        tGCATTTCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                   <  1:1502820/95‑1 (MQ=255)
                                                                                                                             ttCCAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATAAAAATGGAATaaa  >  2:1571092/1‑139 (MQ=255)
                                                                                                                               ccAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                                                  >  2:1265418/1‑41 (MQ=255)
                                                                                                                               ccAGGATACTCCCCCCCCTGGCTATTGTGCGCTCATACACt                                                                                                  <  1:1265418/41‑1 (MQ=255)
                                                                                                                                         |                                                                                                                               
TGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAA  >  NC_000913/2462744‑2463008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: