Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,765,829 (C)5→4 intergenic (‑53/‑89) yfjM ← / → rnlA CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,765,8250C.98.8% 326.9 / ‑2.7 81intergenic (‑49/‑93)yfjM/rnlACP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (36/44);  total (37/44)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

TCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTC  >  NC_000913/2765686‑2765953
                                                                                                                                           |                                                                                                                                 
tCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGc                                                                                                                                   >  2:1588655/1‑139 (MQ=255)
   tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACTGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccc                                                                                                                                 >  1:350605/1‑139 (MQ=255)
   tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccc                                                                                                                                 >  2:105449/1‑139 (MQ=255)
   tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccc                                                                                                                                 >  2:1624539/1‑139 (MQ=255)
    gTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGc                                                                                                                                   <  2:1639458/136‑1 (MQ=255)
    gTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGc                                                                                                                                   >  1:1639458/1‑136 (MQ=255)
               aataatTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTgg                                                                                                                    <  1:1624539/139‑1 (MQ=255)
                        tAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                          <  1:1588655/139‑1 (MQ=255)
                          aGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGc                                                                                                         >  2:89612/1‑139 (MQ=255)
                           gTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCa                                                                                                        <  2:665062/139‑1 (MQ=255)
                           gTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCa                                                                                                        <  1:155814/139‑1 (MQ=255)
                            tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                          <  1:1689931/136‑1 (MQ=255)
                            tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                          >  2:1689931/1‑136 (MQ=255)
                             aTCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCaaa                                                                                                      >  2:828640/1‑139 (MQ=255)
                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccactattc                                                                                                                           >  1:457626/1‑111 (MQ=255)
                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTc                                                                                                                          <  2:457626/118‑1 (MQ=255)
                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATc                                                                                                    >  1:533644/1‑139 (MQ=255)
                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATc                                                                                                    >  1:1214609/1‑139 (MQ=255)
                                 aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCaa                                                                                                  <  2:530306/139‑1 (MQ=255)
                                   ttGGTGCTTGGCGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                <  2:1163543/139‑1 (MQ=255)
                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                <  1:105449/139‑1 (MQ=255)
                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                <  2:350605/139‑1 (MQ=255)
                                     gctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCAGTAGACGATCCTGCCCTATAGTTGGATTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                                                              >  2:1092460/1‑139 (MQ=255)
                                      ctgctTGGTGAGATCCGGAAGAGGTTACCGAAACGATGGACATCAACTCCCATTTTGTGGACTGCCCCCCCCGTAGACGATCCTGCCCCATAGTTGGAGTGCCCCGTTTCAGGTGGCTATATTTAGCAAATCAATacac                                                                                             >  1:1171259/1‑139 (MQ=255)
                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                             >  2:55358/1‑139 (MQ=255)
                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                             >  1:179150/1‑139 (MQ=255)
                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                             >  1:1720372/1‑139 (MQ=255)
                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                <  2:958786/136‑1 (MQ=255)
                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                >  1:958786/1‑136 (MQ=255)
                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                   <  2:818575/139‑1 (MQ=255)
                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                       <  1:828640/139‑1 (MQ=255)
                                                             gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATACTTGGAGCGCCCCTATCCAAGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTagct                                                                      >  1:818575/1‑137 (MQ=255)
                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                          <  1:48527/101‑1 (MQ=255)
                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                          >  2:48527/1‑101 (MQ=255)
                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 <  2:1453845/126‑1 (MQ=255)
                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 >  1:1423047/1‑126 (MQ=255)
                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 >  1:1453845/1‑126 (MQ=255)
                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 <  2:1423047/126‑1 (MQ=255)
                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 >  2:174678/1‑126 (MQ=255)
                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 <  1:174678/126‑1 (MQ=255)
                                                                 ccGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTTGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                >  2:1396377/1‑109 (MQ=255)
                                                                 ccGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                <  1:1396377/109‑1 (MQ=255)
                                                                 ccGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTc                                                                  <  2:179150/139‑1 (MQ=255)
                                                                 ccGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTc                                                                  >  1:1088969/1‑139 (MQ=255)
                                                                      aCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                          <  1:967650/94‑1 (MQ=255)
                                                                      aCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAg                                                                                                          >  2:967650/1‑94 (MQ=255)
                                                                          tGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGt                                                         <  1:239395/139‑1 (MQ=255)
                                                                           ggACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                >  2:108958/1‑99 (MQ=255)
                                                                           ggACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                <  1:108958/99‑1 (MQ=255)
                                                                               atcatcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa                                                    <  2:1720372/139‑1 (MQ=255)
                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 >  1:1272132/1‑108 (MQ=255)
                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 <  2:1272132/108‑1 (MQ=255)
                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                  <  1:89612/139‑1 (MQ=255)
                                                                                             tgtgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaaa                                     <  2:1088969/139‑1 (MQ=255)
                                                                                             tgtgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaaa                                     <  1:55358/139‑1 (MQ=255)
                                                                                               tgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATg                                   <  2:1214609/139‑1 (MQ=255)
                                                                                                ggACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCat                                                             >  2:1420125/1‑113 (MQ=255)
                                                                                                ggACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCat                                                             <  1:1420125/113‑1 (MQ=255)
                                                                                                ggACTGCCCCCACGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGa                                  <  2:1171259/139‑1 (MQ=255)
                                                                                                 gACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGttt                                                                   <  2:272538/106‑1 (MQ=255)
                                                                                                 gACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGttt                                                                   >  1:272538/1‑106 (MQ=255)
                                                                                                    tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGGAAATGACaa                               >  2:1746843/1‑139 (MQ=255)
                                                                                                     gCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                                                        <  2:1222571/81‑1 (MQ=255)
                                                                                                     gCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                                                        >  1:1222571/1‑81 (MQ=255)
                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑‑GGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAgg                          >  2:1559380/1‑139 (MQ=255)
                                                                                                                aGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 <  1:970397/77‑1 (MQ=255)
                                                                                                                aGACGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 >  2:970397/1‑77 (MQ=255)
                                                                                                                  aCGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                >  2:113335/1‑60 (MQ=255)
                                                                                                                  aCGATCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                <  1:113335/60‑1 (MQ=255)
                                                                                                                      tCCTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACtt            >  1:520497/1‑139 (MQ=255)
                                                                                                                       ccTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                      >  2:1476876/1‑65 (MQ=255)
                                                                                                                       ccTGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                      <  1:1476876/65‑1 (MQ=255)
                                                                                                                         tGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                >  2:1176185/1‑53 (MQ=255)
                                                                                                                         tGCCCTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                <  1:1176185/53‑1 (MQ=255)
                                                                                                                           cccTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCTTATGTTTCTATGGGATCCAGGaaa                                     >  1:1182276/1‑109 (MQ=255)
                                                                                                                           cccTATAGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTATCTATGGGATCCAGGaaa                                     <  2:1182276/109‑1 (MQ=255)
                                                                                                                                taGTTGGATTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc  <  1:1092460/139‑1 (MQ=255)
                                                                                                                                taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                                                         >  1:378320/1‑53 (MQ=255)
                                                                                                                                taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                                                         <  2:378320/53‑1 (MQ=255)
                                                                                                                                taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATg                                   <  1:932918/106‑1 (MQ=255)
                                                                                                                                taGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATg                                   >  2:932918/1‑106 (MQ=255)
                                                                                                                                 aGTTGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc  <  1:1746843/139‑1 (MQ=255)
                                                                                                                                   ttGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAAt        >  2:1656572/1‑130 (MQ=255)
                                                                                                                                   ttGGAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAAt        <  1:1656572/130‑1 (MQ=255)
                                                                                                                                     ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 <  1:111026/56‑1 (MQ=255)
                                                                                                                                     ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                 >  2:111026/1‑56 (MQ=255)
                                                                                                                                     ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTa                                                                         >  1:1746660/1‑64 (MQ=255)
                                                                                                                                     ggAGTGCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTa                                                                         <  2:1746660/64‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                 
TCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTC  >  NC_000913/2765686‑2765953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: