Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,238,562 (T)7→6 intergenic (+265/‑18) ygjR → / → alx putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,238,5560T.100.0% 335.8 / NA 82intergenic (+259/‑24)ygjR/alxputative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (37/45);  total (37/45)

TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCAC  >  NC_000913/3238418‑3238679
                                                                                                                                          |                                                                                                                           
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                             >  2:777805/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                             >  2:591915/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                             >  2:1421085/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                             >  2:1412100/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                             >  2:1327131/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                             >  2:1017690/1‑139 (MQ=255)
tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                                                                                             >  1:436999/1‑139 (MQ=255)
 gtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtt                                                                                                                            >  1:352039/1‑139 (MQ=255)
  tgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttt                                                                                                                           >  1:10557/1‑139 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                        <  1:1017690/139‑1 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                        <  2:1055811/139‑1 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                        <  2:10557/139‑1 (MQ=255)
     aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                                                                                        <  1:28450/139‑1 (MQ=255)
        aaCACATCCGGTCACCGGGCATCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttatg                                                                                                                     <  1:1085622/139‑4 (MQ=255)
        aaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttatg                                                                                                                     >  2:614672/1‑136 (MQ=255)
        aaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttatg                                                                                                                     <  1:317058/139‑4 (MQ=255)
          cacaTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTAtgtg                                                                                                                  >  1:860563/1‑139 (MQ=255)
          cacaTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTAtgtg                                                                                                                  >  2:1458377/1‑139 (MQ=255)
            caTCCGGCCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                >  2:1117378/1‑139 (MQ=255)
               ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAgg                                                                                                             <  1:1458377/139‑1 (MQ=255)
               ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAgg                                                                                                             <  1:656350/139‑1 (MQ=255)
               ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAgg                                                                                                             >  1:64655/1‑139 (MQ=255)
                cGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGa                                                                                                            >  2:1540650/1‑139 (MQ=255)
                 ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                           <  1:91686/139‑1 (MQ=255)
                 ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                           <  1:1036889/139‑1 (MQ=255)
                  gTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGCAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAAc                                                                                                          >  1:1409105/1‑139 (MQ=255)
                   tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         <  1:1421085/139‑1 (MQ=255)
                   tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         <  2:860563/139‑1 (MQ=255)
                   tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGCAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         <  2:1409105/139‑1 (MQ=255)
                         ggCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATg                                                                                                   >  1:609859/1‑139 (MQ=255)
                           cAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGaa                                                                                                 <  1:614672/139‑1 (MQ=255)
                              cccGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATAc                                                                                              <  1:1412100/139‑1 (MQ=255)
                                      gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                      <  1:201066/139‑1 (MQ=255)
                                      gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                      <  2:609859/139‑1 (MQ=255)
                                         tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc                                                                                   <  2:815186/139‑1 (MQ=255)
                                                aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAAc                                                                                                          <  1:1207738/109‑1 (MQ=255)
                                                aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAAc                                                                                                          >  2:1207738/1‑109 (MQ=255)
                                                aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                      >  2:572274/1‑129 (MQ=255)
                                                aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                      <  1:572274/129‑1 (MQ=255)
                                                aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCt                                                                            >  2:1466507/1‑139 (MQ=255)
                                                 cGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTa                                                                           <  1:777805/139‑1 (MQ=255)
                                                   tATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATg                                                                         >  2:990701/1‑139 (MQ=255)
                                                     ttCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCt                                                                                                      >  1:441795/1‑108 (MQ=255)
                                                     ttCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCt                                                                                                      <  2:441795/108‑1 (MQ=255)
                                                     ttCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATggg                                                                       >  1:395279/1‑139 (MQ=255)
                                                           ttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGAt                                                                 >  2:629045/1‑139 (MQ=255)
                                                             ttgttgTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                <  1:681307/90‑1 (MQ=255)
                                                             ttgttgTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                >  2:681307/1‑90 (MQ=255)
                                                             ttgttgTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         <  1:25639/97‑1 (MQ=255)
                                                             ttgttgTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         >  2:25639/1‑97 (MQ=255)
                                                                 tgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtg                                                           <  2:653297/139‑1 (MQ=255)
                                                                   tAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATgggg                                                                      <  2:1459167/126‑1 (MQ=255)
                                                                   tAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATgggg                                                                      >  1:1459167/1‑126 (MQ=255)
                                                                     aGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgt                                                       >  2:643757/1‑139 (MQ=255)
                                                                        gagaCCTTGCCGGAAGGCGAGGTTTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                    <  1:1117378/139‑1 (MQ=255)
                                                                        gagaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                    <  2:64655/139‑1 (MQ=255)
                                                                          gaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAAc                                                                                                          >  2:612931/1‑83 (MQ=255)
                                                                          gaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAAc                                                                                                          <  1:612931/83‑1 (MQ=255)
                                                                           aCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGc                                                 >  1:97940/1‑139 (MQ=255)
                                                                               tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCAtt                                             >  2:850267/1‑139 (MQ=255)
                                                                                gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATAc                                                                                              <  1:1503939/89‑1 (MQ=255)
                                                                                gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATAc                                                                                              >  2:1503939/1‑89 (MQ=255)
                                                                                      aGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         >  1:1453600/1‑72 (MQ=255)
                                                                                      aGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         <  2:1453600/72‑1 (MQ=255)
                                                                                           aGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         >  1:836382/1‑67 (MQ=255)
                                                                                           aGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         <  2:836382/67‑1 (MQ=255)
                                                                                            ggTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCg                                <  2:395279/139‑1 (MQ=255)
                                                                                             gTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                               <  1:591915/139‑1 (MQ=255)
                                                                                             gTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                               <  2:97940/139‑1 (MQ=255)
                                                                                             gTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                               <  1:1466507/139‑1 (MQ=255)
                                                                                              tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                              <  1:629045/139‑1 (MQ=255)
                                                                                                  tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                           >  1:231693/1‑58 (MQ=255)
                                                                                                  tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGaa                                                                                                           <  2:231693/58‑1 (MQ=255)
                                                                                                  tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgt                          >  2:1257073/1‑139 (MQ=255)
                                                                                                       aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGttgttg                     >  2:586822/1‑139 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttat                                                                                                                      <  1:889797/42‑3 (MQ=255)
                                                                                                        aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttat                                                                                                                      >  2:889797/1‑40 (MQ=255)
                                                                                                         aaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCa                   <  1:850267/139‑1 (MQ=255)
                                                                                                            gcagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                >  2:1064330/1‑43 (MQ=38)
                                                                                                            gcagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTa                                                                                                                <  1:1064330/43‑1 (MQ=38)
                                                                                                            gcagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAggg                <  1:990701/139‑1 (MQ=255)
                                                                                                             cagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         >  1:344722/1‑49 (MQ=39)
                                                                                                             cagcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACt                                                                                                         <  2:344722/49‑1 (MQ=39)
                                                                                                               gcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTc                                                                                                       <  2:1001444/49‑1 (MQ=39)
                                                                                                               gcGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTc                                                                                                       >  1:1001444/1‑49 (MQ=39)
                                                                                                                cGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCgg                                                                                        >  1:668563/1‑63 (MQ=255)
                                                                                                                cGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCgg                                                                                        <  2:668563/63‑1 (MQ=255)
                                                                                                                cGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGa                                                                  >  1:1317867/1‑85 (MQ=255)
                                                                                                                cGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGa                                                                  <  2:1317867/85‑1 (MQ=255)
                                                                                                                cGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                               <  2:698970/120‑1 (MQ=255)
                                                                                                                cGGCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                               >  1:698970/1‑120 (MQ=255)
                                                                                                                 ggCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                              <  1:138200/120‑1 (MQ=255)
                                                                                                                 ggCTGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                              >  2:138200/1‑120 (MQ=255)
                                                                                                                    tGACGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTg        <  2:436999/139‑1 (MQ=255)
                                                                                                                      aCGTCTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTggg      >  2:812786/1‑139 (MQ=255)
                                                                                                                          cTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                      >  1:1495978/1‑55 (MQ=255)
                                                                                                                          cTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                      <  2:1495978/55‑1 (MQ=255)
                                                                                                                          cTTCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGcac  <  2:352039/139‑1 (MQ=255)
                                                                                                                           ttCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtg                                                           >  2:797976/1‑81 (MQ=255)
                                                                                                                           ttCCGACGTTGGCCGTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtg                                                           <  1:797976/81‑1 (MQ=255)
                                                                                                                                          |                                                                                                                           
TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCAC  >  NC_000913/3238418‑3238679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: