| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,399,985 | (C)7→8 | coding (252/516 nt) | ogt ← | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,399,978 | 1 | . | C | 97.9% | 164.8 / ‑3.4 | 48 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (21/26); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
ATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399844‑1400111 | aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cccc > 2:996197/1‑139 (MQ=255)aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑cccc < 1:863679/139‑1 (MQ=255) ttCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccc < 1:996197/139‑1 (MQ=255) aTCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccc‑cccg < 1:1294309/139‑3 (MQ=255) cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccc‑cccgtag < 2:705655/139‑6 (MQ=255) cGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCCCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑ccccc‑cccgtag < 1:1536610/139‑6 (MQ=255) gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGc < 2:10197/139‑1 (MQ=255) cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTggg > 1:300217/1‑139 (MQ=255) gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGa < 2:300217/139‑1 (MQ=255) cACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGaa < 1:96322/139‑1 (MQ=255) aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAg < 2:212936/139‑1 (MQ=255) cGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTAt < 2:193996/139‑1 (MQ=255) gCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCATTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCa < 1:799123/139‑1 (MQ=255) aCGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCaat < 1:524382/139‑1 (MQ=255) gcgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGt > 1:1514622/1‑131 (MQ=255) gcgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGt < 2:1514622/131‑1 (MQ=255) cgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCaata > 2:1455789/1‑137 (MQ=255) cgccgcgccAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCaata < 1:1455789/137‑1 (MQ=255) gCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGc < 2:1719792/139‑1 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa > 2:1326493/1‑139 (MQ=255) cccAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCa < 1:358763/139‑1 (MQ=255) cAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCa > 2:868903/1‑93 (MQ=255) cAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCa < 1:868903/93‑1 (MQ=255) tGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTg > 2:1072759/1‑139 (MQ=255) tgtaTTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTaaa < 2:891302/136‑1 (MQ=255) aCCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCt < 1:417292/139‑1 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAata > 2:157596/1‑107 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAata < 1:157596/107‑1 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAt > 2:308987/1‑139 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAt > 1:1301906/1‑139 (MQ=255) gCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAt > 2:1315817/1‑139 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAg < 2:319691/73‑1 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAg > 1:319691/1‑73 (MQ=255) ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtg > 2:463753/1‑139 (MQ=255) gggATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCa < 1:1697825/139‑1 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGa > 2:1728136/1‑57 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGa < 1:1728136/57‑1 (MQ=255) gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt > 2:1385754/1‑103 (MQ=255) gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 1:1385754/103‑1 (MQ=255) cGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcgc < 1:308987/139‑1 (MQ=255) aCTTCGCGCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTg > 1:1300962/1‑139 (MQ=255) cgcgCTGAAATGGCGTCCCCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGACTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGa > 1:32811/1‑139 (MQ=255) cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCaa < 1:1063425/46‑1 (MQ=255) cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCaa > 2:1063425/1‑46 (MQ=255) cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCg < 2:973098/96‑1 (MQ=255) cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCg > 1:973098/1‑96 (MQ=255) cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgc > 2:249342/1‑118 (MQ=255) cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgc < 1:249342/118‑1 (MQ=255) cgcgCTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt > 2:1526833/1‑139 (MQ=255) cacgcTGAAATGGCGT‑‑CCCCCTCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTGAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt > 1:1079211/3‑139 (MQ=255) gcgcTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt < 2:1501196/82‑1 (MQ=255) gcgcTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt > 1:1501196/1‑82 (MQ=255) cgcTGAAATGGCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATaa < 1:1326493/139‑1 (MQ=255) ggCGTC‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc < 1:463753/139‑1 (MQ=255) | ATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑‑CCCCC‑CCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399844‑1400111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |