Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,970,032 (G)6→7 coding (1002/1047 nt) wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,970,0261.G100.0% 162.7 / NA 45V332V (GTC→GTGwzzEEntobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (23/22);  total (23/22)

GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATT  >  NC_000913/3969888‑3970152
                                                                                                                                           |                                                                                                                              
ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc                                                                                                                                 >  2:1789335/1‑139 (MQ=255)
ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTc                                                                                                                                 >  2:605502/1‑139 (MQ=255)
     tGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCggggg                                                                                                                           <  1:1789335/139‑1 (MQ=255)
       ttAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCggggggg                                                                                                                         <  2:1505462/139‑2 (MQ=255)
          aaCACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCgggggggctg                                                                                                                      >  2:677882/1‑135 (MQ=255)
                  gAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGc                                                                                                               <  1:630801/139‑1 (MQ=255)
                       ttGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGgtg                                                                                                          <  1:1227975/139‑1 (MQ=255)
                       ttGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGgtg                                                                                                          <  2:1452912/139‑1 (MQ=255)
                       ttGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCggggggg                                                                                                                         >  1:796138/1‑122 (MQ=255)
                       ttGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCggggggg                                                                                                                         <  2:796138/123‑2 (MQ=255)
                         ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTc                                                                                                        >  1:1076084/1‑139 (MQ=255)
                         ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCggggggg                                                                                                                         <  1:284844/121‑2 (MQ=255)
                         ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCggggggg                                                                                                                         >  2:284844/1‑120 (MQ=255)
                               aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTa                                                                                                  >  1:1237034/1‑139 (MQ=255)
                                          gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTg                                                                                       >  2:905759/1‑139 (MQ=255)
                                           cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                                                                      >  1:145204/1‑139 (MQ=255)
                                           cgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                                                                      >  1:1366112/1‑139 (MQ=255)
                                            gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                     <  2:145204/139‑1 (MQ=255)
                                                tCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGgtg                                                                                                          <  2:728309/114‑1 (MQ=255)
                                                tCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGgtg                                                                                                          >  1:728309/1‑114 (MQ=255)
                                                  aGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTAGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt                                                                               <  2:1076084/139‑1 (MQ=255)
                                                          cGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCgg                                                                                                                              >  1:1037441/1‑83 (MQ=255)
                                                          cGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCgg                                                                                                                              <  2:1037441/83‑1 (MQ=255)
                                                                ttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGc                                                                                                               >  1:779363/1‑93 (MQ=255)
                                                                ttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGc                                                                                                               <  2:779363/93‑1 (MQ=255)
                                                                 tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCg                                                                >  2:309789/1‑139 (MQ=255)
                                                                   cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGt                                                              >  2:1463639/1‑139 (MQ=255)
                                                                     tACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGa                                                            >  2:787244/1‑139 (MQ=255)
                                                                     tACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGa                                                            >  2:1289842/1‑139 (MQ=255)
                                                                                  ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGgcg                                                 <  2:326651/137‑1 (MQ=255)
                                                                                  ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGgcg                                                 >  1:326651/1‑137 (MQ=255)
                                                                                    ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcg                                             <  1:1463639/139‑1 (MQ=255)
                                                                                     gTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc                                            <  1:905759/139‑1 (MQ=255)
                                                                                                tAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                          <  1:592519/98‑1 (MQ=255)
                                                                                                tAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                          >  2:592519/1‑98 (MQ=255)
                                                                                                      aCGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa                           <  1:677882/139‑1 (MQ=255)
                                                                                                        gtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc                         >  1:1318684/1‑139 (MQ=255)
                                                                                                          cgtGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGa                       <  1:787244/139‑1 (MQ=255)
                                                                                                            tGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                     >  2:1007073/1‑139 (MQ=255)
                                                                                                               cTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                  <  1:309789/139‑1 (MQ=255)
                                                                                                                  ccTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAg               >  1:820742/1‑139 (MQ=255)
                                                                                                                      atgatTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTact           >  1:1262305/1‑139 (MQ=255)
                                                                                                                        gatTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCACCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga         <  2:1237034/139‑1 (MQ=255)
                                                                                                                         atTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCACATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac        <  1:605502/139‑1 (MQ=255)
                                                                                                                          ttATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt                                                                               <  2:17963/67‑1 (MQ=255)
                                                                                                                          ttATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt                                                                               >  1:17963/1‑67 (MQ=255)
                                                                                                                           tATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                                  <  2:1675496/63‑1 (MQ=255)
                                                                                                                           tATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                                  >  1:1675496/1‑63 (MQ=255)
                                                                                                                           tATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg      >  1:590841/1‑139 (MQ=255)
                                                                                                                           tATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATAGATGTGAAAGTactg          <  1:1201864/135‑1 (MQ=255)
                                                                                                                           tATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGAGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactg          >  2:1201864/1‑135 (MQ=255)
                                                                                                                               cggggCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTAtt  <  2:820742/138‑1 (MQ=255)
                                                                                                                                    cATTGTCGGGGGGGCTGATCGGGGCTGGTGTCTCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc                         >  1:1066830/1‑111 (MQ=255)
                                                                                                                                    cATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                               >  2:327747/1‑89 (MQ=255)
                                                                                                                                    cATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                               <  1:327747/89‑1 (MQ=255)
                                                                                                                                    cATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc                         <  2:1066830/111‑1 (MQ=255)
                                                                                                                                           |                                                                                                                              
GGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATT  >  NC_000913/3969888‑3970152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: