New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 60439984 (1.100)3 (0.040) 3/210 7.4 3.4% coding (265/1248 nt) ybdG mechanosensitive channel protein, miniconductance
?NC_000913 604404 = 87 (1.170)coding (260/1248 nt) ybdG mechanosensitive channel protein, miniconductance
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/604259‑604399
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagAATACCGCCTGAATATTGACGATAATCCCCTGCAGGGTAAAAGCTAAACGGTGGAAGAGTTTATTCTGGGTAATGATTTGCAACCAAAGCCGTGAACTGGCGATGGCACGTTTTTCGAAGGTCCGCAGTACCACC  >  NC_000913/604404‑604538
                                                                                                                                                                                                                                                                                    
TAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGC                                                                                                                                           <  2:1568223/139‑1
 AACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCA                                                                                                                                          <  1:148719/139‑1
                                 ATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGC                                                                                                                                           >  1:408513/1‑106
                                 ATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGC                                                                                                                                           <  2:408513/106‑1
                                                             ACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGAATACCGCCTGAATATTGACGATAATCCCCTGCAGGGTAAAAGCTAAACGGTGGAAGAG                                                                              >  1:1625193/1‑139
                                                                  AAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAGAATACCGCCTGAATATTGACGATAATCCCCTGCAGGGTAAAAGCTAAACGGTGGAAGAGTTTAT                                                                         <  2:1625193/139‑1
                                                                        CGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGC                                                                                                                                           >  1:1233146/1‑67
                                                                        CGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGC                                                                                                                                           <  2:1233146/67‑1
                                                                                           AACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGC                                                                                                                                           >  1:882212/1‑48
                                                                                           AACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGC                                                                                                                                           <  2:882212/48‑1
                                                                                                                                         GCAGAATAACGCGTGAATATTGACGATAATCCCCTGCAGGGTAAAAGCTAAACTGGGGAAGAGTTTATTCTGGGTAATGATTTGCAACCAAAGCCGTGAACTGGCGATGGCACGTTTTTCGCAGGTCCGCAGTACCACC  <  1:557463/139‑1
                                                                                                                                                                                                                                                                                    
TAACTGAGATGCTGCCGGGAATTTCTGCGCCAGATTCAAAATAACATCCAGCAACGAGAAGACTGAAAGCAGCGCATACATCATGATCCACAACTGCGCGCAGGTAGTCAGAATATCTGCCGCTTCGGTGCCTTTTTGCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/604259‑604399
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagAATACCGCCTGAATATTGACGATAATCCCCTGCAGGGTAAAAGCTAAACGGTGGAAGAGTTTATTCTGGGTAATGATTTGCAACCAAAGCCGTGAACTGGCGATGGCACGTTTTTCGAAGGTCCGCAGTACCACC  >  NC_000913/604404‑604538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.