| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,857,145 | 0 | T | A | 49.1% | 12.2 / 78.1 | 53 | L18H (CTC→CAC) ‡ | mutS | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base T (13/14); new base A (16/10); total (29/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑AT‑C‑TCAGGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCA > NC_000913/2857034‑2857254 | tACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑t > 2:1591783/1‑139 (MQ=255) aaCGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCct < 1:212355/139‑1 (MQ=255) aaTAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGtt > 1:900540/1‑139 (MQ=255) cATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCctgct < 2:1571033/127‑1 (MQ=255) cATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCctgct > 1:1571033/1‑127 (MQ=255) tCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATgg > 1:657454/1‑139 (MQ=255) tCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATgg > 1:1540159/1‑139 (MQ=255) cacaCCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATggg < 1:1127603/139‑1 (MQ=255) taagatacagatatCAGGGAACCGGACATAACCCCATCAGTGCAATAGAAAACTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTTACATCCCAGCATCCCGATA‑TCCTGCTGTTTTACCGGATGGGTGa < 1:266692/129‑1 (MQ=255) tttAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGAT‑AGTC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTa > 2:78324/1‑139 (MQ=255) tttAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGATTCCTGCTGTTTTACCGGATGGGTGAttttt > 1:1749764/1‑139 (MQ=255) atatCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa < 1:1591783/139‑1 (MQ=255) tatCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGAT‑AGTC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAAc < 2:657454/139‑1 (MQ=255) tatCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAAc < 1:78324/139‑1 (MQ=255) tatCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAAc < 2:699844/139‑1 (MQ=255) atCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCctg < 2:1559857/107‑1 (MQ=255) atCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACt > 1:651683/1‑139 (MQ=255) atCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACt > 1:949114/1‑139 (MQ=255) atCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGAT‑AGTC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCctg > 1:1559857/1‑107 (MQ=255) cAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGt > 1:333787/1‑139 (MQ=255) aGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa > 1:1549140/1‑134 (MQ=255) aGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa < 2:1549140/134‑1 (MQ=255) gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAAc > 1:922291/1‑134 (MQ=255) gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAAc < 2:922291/134‑1 (MQ=255) gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCctgct > 2:1227831/1‑105 (MQ=255) gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCctgct < 1:1227831/105‑1 (MQ=255) gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa > 2:32462/1‑133 (MQ=255) gggAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGaa < 1:32462/133‑1 (MQ=255) gcGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAAcctgtctctta > 2:266692/2‑129 (MQ=255) gACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATg > 1:781458/1‑135 (MQ=255) gACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATg < 2:781458/135‑1 (MQ=255) aTGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAAcgcg > 2:412575/1‑139 (MQ=255) tGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCTAAAcgcgc > 2:790993/1‑139 (MQ=255) gTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATgac < 1:994015/122‑1 (MQ=255) gTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATgac > 2:994015/1‑122 (MQ=255) gTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTc > 1:1499794/1‑139 (MQ=255) tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATgac > 2:852898/1‑121 (MQ=255) tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATgac < 1:852898/121‑1 (MQ=255) tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCg > 1:1470875/1‑139 (MQ=255) tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCg > 1:835188/1‑139 (MQ=255) tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATggg > 2:597758/1‑96 (MQ=255) tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATggg < 1:597758/96‑1 (MQ=255) tGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCg > 2:60983/1‑139 (MQ=255) gCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGc < 2:333787/139‑1 (MQ=255) gCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGc < 1:790993/139‑1 (MQ=255) gAAAATTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGATTCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAActg < 2:1749764/139‑1 (MQ=255) aaaaTTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAActgct < 2:119062/139‑1 (MQ=255) aaaaTTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAActgct < 2:1499794/139‑1 (MQ=255) aaTTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCctgct > 1:653425/1‑71 (MQ=255) aaTTTCGACGCCCATACGCCCATGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCctgct < 2:653425/71‑1 (MQ=255) aaTTTCGACGCCCATACGCCCATGATGCAGCAGT‑GA‑T‑AGTCGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTgg > 2:879407/1‑139 (MQ=255) cgcccatacgcccatGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCa > 2:485047/1‑139 (MQ=255) cgcccatacgcccatGATGCAGCAGTGATAG‑TC‑‑GCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCa < 2:1470875/139‑1 (MQ=255) | TACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAGCAGT‑AT‑C‑TCAGGCTGAAAGCCCAGCATCCCGAGA‑TCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCA > NC_000913/2857034‑2857254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |