Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	50,162	C→T	H114Y (CAT→TAT)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	50,162	0	C	T	100.0%	251.5 / NA	77	H114Y (CAT→TAT)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/38);  total (39/38)

TCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGG  >  NC_000913/50032‑50296                                                                                                                                   |                                                                                                                                       tCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTatat                                                                                                                                    <  2:1397859/135‑1 (MQ=255) tCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTatat                                                                                                                                    >  1:1397859/1‑135 (MQ=255)  cgcgTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGa                                                                                                                                 <  1:421099/137‑1 (MQ=255)  cgcgTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGa                                                                                                                                 >  2:421099/1‑137 (MQ=255)  cgcgTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACg                                                                                                                               <  2:516352/139‑1 (MQ=255)    cgTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCa                                                                                                                             <  1:1556135/139‑1 (MQ=255)    cgTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCa                                                                                                                             >  1:1574935/1‑139 (MQ=255)         cGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGt                                                                                                                        >  1:841234/1‑139 (MQ=255)                   gTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGac                                                                                                              >  1:185040/1‑139 (MQ=255)                      ggTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACAcc                                                                                                           <  2:841234/139‑1 (MQ=255)                             gAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTcc                                                                                                    >  1:1314420/1‑139 (MQ=255)                             gAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTcc                                                                                                    >  2:1753409/1‑139 (MQ=255)                               aGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCg                                                                                                  <  2:1783520/139‑1 (MQ=255)                                  cATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGAt                                                                                               >  1:842034/1‑139 (MQ=255)                                    tCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAGACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTa                                                                                             <  2:681445/139‑1 (MQ=255)                                    tCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTa                                                                                             >  1:1387667/1‑139 (MQ=255)                                     cgcgGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTAc                                                                                            >  1:1332656/1‑139 (MQ=255)                                     cgcgGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTAc                                                                                            >  2:299690/1‑139 (MQ=255)                                        ggcgTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAg                                                                                         <  2:1332656/139‑1 (MQ=255)                                             gtggtgACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGa                                                                                    <  2:1314420/139‑1 (MQ=255)                                             gtggtgACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGa                                                                                    <  1:1753409/139‑1 (MQ=255)                                                gtgACGTACCAGAAATCATGGTGATT‑GCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCa                                                                                                                             >  2:1000307/1‑94 (MQ=255)                                                gtgACGTACCAGAAATCATGGTGATT‑GCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCa                                                                                                                             <  1:1000307/94‑1 (MQ=255)                                                      tACCAGAAAACATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGCGCCGGATGACTGGGa                                                                           >  2:1203463/1‑139 (MQ=255)                                                       aCCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGaa                                                                          >  2:354651/1‑139 (MQ=255)                                                        ccAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGac                                                                                                              >  1:911904/1‑102 (MQ=255)                                                        ccAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGac                                                                                                              <  2:911904/102‑1 (MQ=255)                                                                 aTGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACg                                                                                                                               >  1:997436/1‑76 (MQ=255)                                                                 aTGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACg                                                                                                                               <  2:997436/76‑1 (MQ=255)                                                                     tGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGa                                                            <  1:184858/139‑1 (MQ=255)                                                                      gATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGaa                                                           >  2:403981/1‑139 (MQ=255)                                                                       aTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGc                                                              <  2:624793/135‑1 (MQ=255)                                                                       aTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGc                                                              >  1:624793/1‑135 (MQ=255)                                                                         tGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTc                                                        >  1:170768/1‑139 (MQ=255)                                                                         tGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTc                                                        >  2:1630710/1‑139 (MQ=255)                                                                           gcggcgGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCa                                                      <  1:507549/139‑1 (MQ=255)                                                                           gcggcgGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCa                                                      <  1:299690/139‑1 (MQ=255)                                                                             ggcggTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACg                                                    >  1:708030/1‑139 (MQ=255)                                                                              gcggTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAg                                                                                         <  1:1747825/101‑1 (MQ=255)                                                                              gcggTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAg                                                                                         >  2:1747825/1‑101 (MQ=255)                                                                              gcggTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGa                                                   >  2:1655972/1‑139 (MQ=255)                                                                                     cgTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGa                                                                                    >  1:1231189/1‑99 (MQ=255)                                                                                     cgTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGa                                                                                    <  2:1231189/99‑1 (MQ=255)                                                                                            gAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGACTCGGTATTCAGCGAATTCCACGACGCCGATGCGCAGa                                     <  1:1203463/139‑1 (MQ=255)                                                                                             aaCAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGaa                                    <  2:1380081/139‑1 (MQ=255)                                                                                               cAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAAct                                  >  2:1618093/1‑139 (MQ=255)                                                                                                  ttCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACAccc                                                                                                          <  2:1220885/64‑1 (MQ=255)                                                                                                  ttCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACAccc                                                                                                          >  1:1220885/1‑64 (MQ=255)                                                                                                  ttCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCa                                                      >  1:1206190/1‑116 (MQ=255)                                                                                                  ttCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATgcgc                                        >  2:192115/1‑130 (MQ=255)                                                                                                  ttCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATgcgc                                        <  1:192115/130‑1 (MQ=255)                                                                                                  ttCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACtctc                               >  1:480118/1‑139 (MQ=255)                                                                                                  ttCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCACCGAATTCCa                                                      <  2:1206190/116‑1 (MQ=255)                                                                                                   tCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTca                              <  2:170768/139‑1 (MQ=255)                                                                                                    cTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACg                                                                                                                               >  1:1279613/1‑41 (MQ=255)                                                                                                    cTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACg                                                                                                                               <  2:1279613/41‑1 (MQ=255)                                                                                                    cTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGa                                                   <  2:1422211/117‑1 (MQ=255)                                                                                                    cTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGa                                                   >  1:1422211/1‑117 (MQ=255)                                                                                                    cTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTcac                             >  1:205947/1‑139 (MQ=255)                                                                                                    cTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTcac                             >  1:1124168/1‑139 (MQ=255)                                                                                                          aaaaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTAt                       <  1:1618093/139‑1 (MQ=255)                                                                                                            aaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTggg                                                                            <  2:449669/84‑1 (MQ=255)                                                                                                            aaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTggg                                                                            >  1:449669/1‑84 (MQ=255)                                                                                                            aaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTg                                              >  2:1076872/1‑114 (MQ=255)                                                                                                            aaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTg                                              <  1:1076872/114‑1 (MQ=255)                                                                                                            aaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTa                        >  1:1364321/1‑136 (MQ=255)                                                                                                            aaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTa                        <  2:1364321/136‑1 (MQ=255)                                                                                                               cgcAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTggg                                                                            <  2:620890/81‑1 (MQ=255)                                                                                                               cgcAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTggg                                                                            >  1:620890/1‑81 (MQ=255)                                                                                                               cgcAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCtt                  <  1:403981/139‑1 (MQ=255)                                                                                                                 cAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATgcg                                         >  2:461771/1‑114 (MQ=255)                                                                                                                 cAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATgcg                                         <  1:461771/114‑1 (MQ=255)                                                                                                                 cAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTg                <  1:354651/139‑1 (MQ=255)                                                                                                                          tATCTGACGTATATCGACGCAGAAGTGGAAGGCGa                                                                                                               <  2:258669/35‑1 (MQ=255)                                                                                                                          tATCTGACGTATATCGACGCAGAAGTGGAAGGCGa                                                                                                               >  1:258669/1‑35 (MQ=255)                                                                                                                           aTCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGa      <  1:1655972/139‑1 (MQ=255)                                                                                                                               gACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAgcgg  <  2:205947/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       TCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGG  >  NC_000913/50032‑50296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑