Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	829,972	C→T	M1M (GTG→ATG) † *224* (TAG→TAA)	ybhG ← ybiH ←	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor DUF1956 domain‑containing tetR family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	829,972	0	C	T	100.0%	176.1 / NA	55	M1M (GTG→ATG) † *224* (TAG→TAA)	ybhG ybiH	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor DUF1956 domain‑containing tetR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/26);  total (29/26)

ACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTG  >  NC_000913/829839‑830098                                                                                                                                        |                                                                                                                               aCCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACaa                                                                                                                           >  1:1762223/1‑139 (MQ=255) aCCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACaa                                                                                                                           >  2:1520147/1‑139 (MQ=255)           tatCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGc                                                                                                                 >  1:1578910/1‑139 (MQ=255)               cACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATa                                                                                                             >  2:1527043/1‑139 (MQ=255)                              cGTCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                              >  1:253032/1‑139 (MQ=255)                              cGTCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                              >  1:1123054/1‑139 (MQ=255)                              cGTCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                              >  2:770523/1‑139 (MQ=255)                              cGTCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                              >  2:355409/1‑139 (MQ=255)                                tCAGGCCGTTATCCTGGCGGCTTTGATACCACACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                              >  1:830483/1‑139 (MQ=255)                                tCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAgg                                                                                            <  1:355409/139‑1 (MQ=255)                                      cGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGata                                                                                      <  1:1527043/139‑1 (MQ=255)                                         tATCCTGGCGGCTTCGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCTCCTATCCGATCACGACAGGTTTTTCCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGa                                                                                   <  2:1101537/139‑1 (MQ=255)                                          aTCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGAc                                                                                  <  2:253032/139‑1 (MQ=255)                                                gcggcTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCa                                                                            >  1:279570/1‑139 (MQ=255)                                                   gcTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                         <  2:620199/139‑1 (MQ=255)                                                   gcTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                         <  2:735247/139‑1 (MQ=255)                                                   gcTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                         <  2:1762223/139‑1 (MQ=255)                                                      ttGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCt                                                                      >  1:1307639/1‑139 (MQ=255)                                                       tGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTg                                                                     >  2:1371982/1‑139 (MQ=255)                                                           ac‑‑caccAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTg                                                                 <  2:1726030/139‑1 (MQ=255)                                                               caccaGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGAt                                                               <  1:1707737/139‑1 (MQ=255)                                                                accaGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATc                                                              >  1:1584425/1‑139 (MQ=255)                                                                     tAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTc                                                         >  1:1724403/1‑139 (MQ=255)                                                                     tAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTc                                                         >  1:441724/1‑139 (MQ=255)                                                                      aGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCg                                                        <  2:1578910/139‑1 (MQ=255)                                                                        ccTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGt                                                      <  2:1307639/139‑1 (MQ=255)                                                                         cTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTc                                                     >  2:1039218/1‑139 (MQ=255)                                                                            cGGCAACCACGGCGGTAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                             <  1:554678/96‑1 (MQ=255)                                                                            cGGCAACCACGGCGGTAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                             >  2:554678/1‑96 (MQ=255)                                                                               cAACCACGGCGGCAAGTACCACTACCGCCAATCCGATAACGACTGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGAGATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTttct                                               >  2:1202421/1‑139 (MQ=255)                                                                                  ccACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCa                                            >  2:1360546/1‑139 (MQ=255)                                                                                        cggcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAAc                                      <  1:1360546/139‑1 (MQ=255)                                                                                             aGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGt                                                                                           >  1:1034392/1‑81 (MQ=255)                                                                                             aGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGt                                                                                           <  2:1034392/81‑1 (MQ=255)                                                                                             aGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGt                                 >  2:307932/1‑139 (MQ=255)                                                                                                aCCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCa                              >  1:1660756/1‑139 (MQ=255)                                                                                                aCCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCa                              <  1:1562571/139‑1 (MQ=255)                                                                                                  cACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                            <  1:1371982/139‑1 (MQ=255)                                                                                                  cACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                            <  2:1660756/139‑1 (MQ=255)                                                                                                    cTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCg                          <  2:830483/139‑1 (MQ=255)                                                                                                       ccGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTGGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTa                       <  2:441724/139‑1 (MQ=255)                                                                                                       ccGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCATCGTCTGCTTGATCAGTTCGGTCCTTTCTTCATCGAACGCGGTCCAGCCGGTa                       <  1:1039218/139‑1 (MQ=255)                                                                                                       ccGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTa                       <  2:279570/139‑1 (MQ=255)                                                                                                         gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGa                                                                                   >  2:23565/1‑77 (MQ=255)                                                                                                         gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGa                                                                                   <  1:23565/77‑1 (MQ=255)                                                                                                         gCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACg                     >  2:337391/1‑139 (MQ=255)                                                                                                          ccAATCCGATCACGACAGGTTTTTTCATCATTACAACCTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                             <  2:1200273/66‑1 (MQ=255)                                                                                                          ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                             >  1:1200273/1‑66 (MQ=255)                                                                                                          ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGt                    >  1:146339/1‑139 (MQ=255)                                                                                                            aaTCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTaa                  <  1:991350/139‑1 (MQ=255)                                                                                                                            gTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                         <  2:1555251/68‑1 (MQ=255)                                                                                                                            gTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                         >  1:1555251/1‑68 (MQ=255)                                                                                                                            gTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGt                        <  2:304410/117‑1 (MQ=255)                                                                                                                            gTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGt                        >  1:304410/1‑117 (MQ=255)                                                                                                                            gTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACATAATTGTTTCTTTg  >  1:1187232/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                               ACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATAC‑‑CACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTG  >  NC_000913/829839‑830098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑