Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 875,327 G→A *783* (TAG→TAA yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913875,3270GA100.0% 188.0 / NA 59*783* (TAG→TAAyliEputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/32);  total (27/32)

GAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAGTTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGTTA  >  NC_000913/875196‑875461
                                                                                                                                   |                                                                                                                                      
gAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                                 <  2:449742/139‑1 (MQ=255)
     cGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTc                                                                                                                            <  1:666751/139‑1 (MQ=255)
              cGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCaa                                                                                                                   >  1:1555937/1‑139 (MQ=255)
              cGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCaa                                                                                                                   >  2:1751687/1‑139 (MQ=255)
                aaGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATa                                                                                                                 >  1:114990/1‑139 (MQ=255)
                      ttGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAAtt                                                                                                                                      <  2:715371/112‑1 (MQ=255)
                      ttGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg                                                                                                           <  1:1751687/139‑1 (MQ=255)
                         cgcgcgAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                                 >  2:494444/1‑114 (MQ=255)
                         cgcgcgAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                                 <  1:494444/114‑1 (MQ=255)
                           cgcgAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACtt                                                                                                      >  2:1378850/1‑139 (MQ=255)
                                aTTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTAcagt                                                                                                 <  1:265440/139‑1 (MQ=255)
                                   aaTCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTAcagtcag                                                                                              <  1:1648916/139‑1 (MQ=255)
                                    aTCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAATAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTAcagtcagt                                                                                             <  2:1580943/139‑1 (MQ=255)
                                         aTTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTctgct                                                                                        <  1:874969/139‑1 (MQ=255)
                                                 gtgtTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg                                                                                                           <  2:243429/112‑1 (MQ=255)
                                                 gtgtTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg                                                                                                           >  1:243429/1‑112 (MQ=255)
                                                          cTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGAttt                                                                       <  1:1378850/139‑1 (MQ=255)
                                                             aGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTcag                                                                    <  2:114990/139‑1 (MQ=255)
                                                              ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCa                                                                                                                          >  2:1125517/1‑84 (MQ=255)
                                                              ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCa                                                                                                                          <  1:1125517/84‑1 (MQ=255)
                                                              ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTctg                                                                                          <  2:1602950/116‑1 (MQ=255)
                                                              ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTctg                                                                                          >  1:1602950/1‑116 (MQ=255)
                                                                    tCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCa                                                                                                                          >  1:456041/1‑78 (MQ=255)
                                                                    tCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCa                                                                                                                          <  2:456041/78‑1 (MQ=255)
                                                                    tCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGc                                                             >  2:1407049/1‑139 (MQ=255)
                                                                         aCCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCg                                                        >  1:975937/1‑139 (MQ=255)
                                                                          ccAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCt                                                           <  1:1248536/135‑1 (MQ=255)
                                                                          ccAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCt                                                           >  2:1248536/1‑135 (MQ=255)
                                                                          ccAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTATGCTGATTTTAATCATGATTTCAGCAGTTGCCTGCgg                                                       >  1:1145132/1‑139 (MQ=255)
                                                                           cAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCggg                                                      <  1:895255/139‑1 (MQ=255)
                                                                              aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGtt                                                                                                             <  2:1488346/81‑1 (MQ=255)
                                                                              aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGtt                                                                                                             >  1:1488346/1‑81 (MQ=255)
                                                                              aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATc                                                   >  2:558620/1‑139 (MQ=255)
                                                                                aCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCAt                                                                            <  2:788908/112‑1 (MQ=255)
                                                                                aCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCAt                                                                            >  1:788908/1‑112 (MQ=255)
                                                                                  ccATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTaca                                               >  2:170198/1‑139 (MQ=255)
                                                                                  ccATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTaca                                               >  1:1016080/1‑139 (MQ=255)
                                                                                        cATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCaaa                                         <  2:1555937/139‑1 (MQ=255)
                                                                                          ttCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATg                                       >  1:465644/1‑139 (MQ=255)
                                                                                            ctcCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGt                                     <  2:490693/139‑1 (MQ=255)
                                                                                            ctcCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGt                                     >  1:490693/1‑139 (MQ=255)
                                                                                               cGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATc                                                                              <  2:1527894/95‑1 (MQ=255)
                                                                                               cGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATc                                                                              >  1:1527894/1‑95 (MQ=255)
                                                                                                 ccTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg                                <  1:170198/139‑1 (MQ=255)
                                                                                                    gggATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCCATAAGTTCGTACTTACAGTCAGTGTGCTGATTTTTATCATGATTTCAGCACTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAAc                             <  2:1145132/139‑1 (MQ=255)
                                                                                                      gATAAAAGTGGAAAGTTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACgg                           >  2:1046226/1‑139 (MQ=255)
                                                                                                      gATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg                                >  2:537019/1‑134 (MQ=255)
                                                                                                      gATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg                                <  1:537019/134‑1 (MQ=255)
                                                                                                           aaGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGta                      <  2:1632585/139‑1 (MQ=255)
                                                                                                                gAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTaaa                                  <  2:1253516/122‑1 (MQ=255)
                                                                                                                gAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTaaa                                  >  1:1253516/1‑122 (MQ=255)
                                                                                                                gAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGcc                 >  2:822876/1‑139 (MQ=255)
                                                                                                                 aaaaTTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg                                <  2:1417669/123‑1 (MQ=255)
                                                                                                                 aaaaTTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg                                >  1:1417669/1‑123 (MQ=255)
                                                                                                                       aGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACa          >  2:1605567/1‑139 (MQ=255)
                                                                                                                       aGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACa          <  2:1016080/139‑1 (MQ=255)
                                                                                                                         tAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGt        <  1:822876/139‑1 (MQ=255)
                                                                                                                             agagTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGt    <  1:558620/139‑1 (MQ=255)
                                                                                                                              gagTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGtt   <  2:465644/139‑1 (MQ=255)
                                                                                                                               agtaatTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGaa                              >  1:664126/1‑111 (MQ=255)
                                                                                                                               agtaatTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGaa                              <  2:664126/111‑1 (MQ=255)
                                                                                                                               agtaatTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGTTa  <  1:1605567/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
GAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAGTTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGTTA  >  NC_000913/875196‑875461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: