Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 875,327 | G→A | *783* (TAG→TAA) | yliE → | putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 875,327 | 0 | G | A | 100.0% | 188.0 / NA | 59 | *783* (TAG→TAA) | yliE | putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/32); total (27/32) |
GAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAGTTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGTTA > NC_000913/875196‑875461 | gAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt < 2:449742/139‑1 (MQ=255) cGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTc < 1:666751/139‑1 (MQ=255) cGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCaa > 1:1555937/1‑139 (MQ=255) cGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCaa > 2:1751687/1‑139 (MQ=255) aaGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATa > 1:114990/1‑139 (MQ=255) ttGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAAtt < 2:715371/112‑1 (MQ=255) ttGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg < 1:1751687/139‑1 (MQ=255) cgcgcgAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt > 2:494444/1‑114 (MQ=255) cgcgcgAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt < 1:494444/114‑1 (MQ=255) cgcgAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACtt > 2:1378850/1‑139 (MQ=255) aTTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTAcagt < 1:265440/139‑1 (MQ=255) aaTCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTAcagtcag < 1:1648916/139‑1 (MQ=255) aTCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAATAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTAcagtcagt < 2:1580943/139‑1 (MQ=255) aTTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTctgct < 1:874969/139‑1 (MQ=255) gtgtTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg < 2:243429/112‑1 (MQ=255) gtgtTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCg > 1:243429/1‑112 (MQ=255) cTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGAttt < 1:1378850/139‑1 (MQ=255) aGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTcag < 2:114990/139‑1 (MQ=255) ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCa > 2:1125517/1‑84 (MQ=255) ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCa < 1:1125517/84‑1 (MQ=255) ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTctg < 2:1602950/116‑1 (MQ=255) ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTctg > 1:1602950/1‑116 (MQ=255) tCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCa > 1:456041/1‑78 (MQ=255) tCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCa < 2:456041/78‑1 (MQ=255) tCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGc > 2:1407049/1‑139 (MQ=255) aCCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCg > 1:975937/1‑139 (MQ=255) ccAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCt < 1:1248536/135‑1 (MQ=255) ccAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCt > 2:1248536/1‑135 (MQ=255) ccAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTATGCTGATTTTAATCATGATTTCAGCAGTTGCCTGCgg > 1:1145132/1‑139 (MQ=255) cAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCggg < 1:895255/139‑1 (MQ=255) aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGtt < 2:1488346/81‑1 (MQ=255) aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGtt > 1:1488346/1‑81 (MQ=255) aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATc > 2:558620/1‑139 (MQ=255) aCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCAt < 2:788908/112‑1 (MQ=255) aCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCAt > 1:788908/1‑112 (MQ=255) ccATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTaca > 2:170198/1‑139 (MQ=255) ccATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTaca > 1:1016080/1‑139 (MQ=255) cATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCaaa < 2:1555937/139‑1 (MQ=255) ttCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATg > 1:465644/1‑139 (MQ=255) ctcCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGt < 2:490693/139‑1 (MQ=255) ctcCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGt > 1:490693/1‑139 (MQ=255) cGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATc < 2:1527894/95‑1 (MQ=255) cGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATc > 1:1527894/1‑95 (MQ=255) ccTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg < 1:170198/139‑1 (MQ=255) gggATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCCATAAGTTCGTACTTACAGTCAGTGTGCTGATTTTTATCATGATTTCAGCACTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAAc < 2:1145132/139‑1 (MQ=255) gATAAAAGTGGAAAGTTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACgg > 2:1046226/1‑139 (MQ=255) gATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg > 2:537019/1‑134 (MQ=255) gATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg < 1:537019/134‑1 (MQ=255) aaGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGta < 2:1632585/139‑1 (MQ=255) gAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTaaa < 2:1253516/122‑1 (MQ=255) gAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTaaa > 1:1253516/1‑122 (MQ=255) gAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGcc > 2:822876/1‑139 (MQ=255) aaaaTTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg < 2:1417669/123‑1 (MQ=255) aaaaTTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg > 1:1417669/1‑123 (MQ=255) aGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACa > 2:1605567/1‑139 (MQ=255) aGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACa < 2:1016080/139‑1 (MQ=255) tAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGt < 1:822876/139‑1 (MQ=255) agagTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGt < 1:558620/139‑1 (MQ=255) gagTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGtt < 2:465644/139‑1 (MQ=255) agtaatTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGaa > 1:664126/1‑111 (MQ=255) agtaatTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGaa < 2:664126/111‑1 (MQ=255) agtaatTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGTTa < 1:1605567/139‑1 (MQ=255) | GAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAGTTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCCGTTA > NC_000913/875196‑875461 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |