Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 983,623 G→A *183* (TAG→TAA ycbK → M15A protease‑related family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913983,6230GA97.5% 249.5 / ‑2.1 81*183* (TAG→TAAycbKM15A protease‑related family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (37/42);  total (38/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

CCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATG  >  NC_000913/983497‑983752
                                                                                                                              |                                                                                                                                 
ccATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAAc                                                                                                                       <  2:151788/139‑1 (MQ=255)
        aaGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgg                                                                                                               >  1:496568/1‑139 (MQ=255)
          ggTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             <  2:941847/139‑1 (MQ=255)
          ggTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             <  2:116742/139‑1 (MQ=255)
          ggTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             <  2:1148113/139‑1 (MQ=255)
          ggTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             <  1:32289/139‑1 (MQ=255)
             aTCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAg                                                                                                          >  1:1237919/1‑139 (MQ=255)
              tCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGt                                                                                                         <  1:1340094/139‑1 (MQ=255)
               cgcgTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTa                                                                                                        <  2:496568/139‑1 (MQ=255)
                   ttAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGaa                                                                                                    <  2:321879/139‑1 (MQ=255)
                           tatTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTG‑‑‑GTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGtatta                                                                                         >  2:1584014/1‑139 (MQ=255)
                                     gCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTg                                                                                                                             <  2:1577756/96‑1 (MQ=255)
                                     gCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTg                                                                                                                             >  1:1577756/1‑96 (MQ=255)
                                     gCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTaa                                                                                                                        <  2:801519/101‑1 (MQ=255)
                                     gCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTaa                                                                                                                        >  1:801519/1‑101 (MQ=255)
                                         cgTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             <  2:56386/108‑1 (MQ=255)
                                         cgTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             >  1:56386/1‑108 (MQ=255)
                                             aTCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                          <  2:1707579/139‑1 (MQ=255)
                                             aTCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                          <  2:1755497/139‑1 (MQ=255)
                                                    cgcgcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCa                                                                   >  2:1495526/1‑139 (MQ=255)
                                                      cgcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTG‑‑‑GTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACt                                                              <  1:1584014/139‑1 (MQ=255)
                                                       gcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                                <  1:1495526/139‑1 (MQ=255)
                                                        cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             <  2:1646288/93‑1 (MQ=255)
                                                        cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             >  1:1454014/1‑93 (MQ=255)
                                                        cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             <  2:1454014/93‑1 (MQ=255)
                                                        cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             <  1:825248/93‑1 (MQ=255)
                                                        cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             >  2:825248/1‑93 (MQ=255)
                                                        cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             >  1:1646288/1‑93 (MQ=255)
                                                        cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAggg                                                                                                              <  2:53883/92‑1 (MQ=255)
                                                        cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAggg                                                                                                              >  1:53883/1‑92 (MQ=255)
                                                        cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAAc                                                               >  2:169650/1‑139 (MQ=255)
                                                        cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAAc                                                               >  1:229284/1‑139 (MQ=255)
                                                         aGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACt                                                              <  2:1237919/139‑1 (MQ=255)
                                                         aGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACt                                                              <  2:1748882/139‑1 (MQ=255)
                                                                      tattatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                                 <  2:836285/91‑1 (MQ=255)
                                                                      tattatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                                 >  1:836285/1‑91 (MQ=255)
                                                                         tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             <  1:1609419/76‑1 (MQ=255)
                                                                         tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                             >  2:1609419/1‑76 (MQ=255)
                                                                         tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATg                                                                                                      <  1:1248998/83‑1 (MQ=255)
                                                                         tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATg                                                                                                      >  2:1248998/1‑83 (MQ=255)
                                                                         tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                          >  2:756956/1‑111 (MQ=255)
                                                                         tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                          <  1:756956/111‑1 (MQ=255)
                                                                         tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                                >  1:684322/1‑121 (MQ=255)
                                                                         tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                                <  2:684322/121‑1 (MQ=255)
                                                                              aCGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                          >  1:1347773/1‑106 (MQ=255)
                                                                              aCGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                          <  2:1347773/106‑1 (MQ=255)
                                                                              aCGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAAc                                         >  1:208951/1‑139 (MQ=255)
                                                                                gtagtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAAc                                                                                                                       >  1:1784685/1‑59 (MQ=255)
                                                                                gtagtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAAc                                                                                                                       <  2:1784685/59‑1 (MQ=255)
                                                                                            tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGaa                                                                                                    >  1:868150/1‑66 (MQ=255)
                                                                                            tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGaa                                                                                                    <  2:868150/66‑1 (MQ=255)
                                                                                            tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAAcc                                    <  1:296699/130‑1 (MQ=255)
                                                                                            tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAAcc                                    >  2:296699/1‑130 (MQ=255)
                                                                                              cATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTcc                                                                     <  2:235792/95‑1 (MQ=255)
                                                                                              cATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTcc                                                                     >  1:235792/1‑95 (MQ=255)
                                                                                               atatTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCa                        <  1:169650/139‑1 (MQ=255)
                                                                                                 atTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                          <  2:732766/87‑1 (MQ=255)
                                                                                                 atTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                          >  1:732766/1‑87 (MQ=255)
                                                                                                      tACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGaaa                                                                                                                   >  2:1612761/1‑41 (MQ=255)
                                                                                                      tACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGaaa                                                                                                                   <  1:1612761/41‑1 (MQ=255)
                                                                                                      tACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                                 <  1:916152/59‑1 (MQ=255)
                                                                                                      tACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                                 >  2:916152/1‑59 (MQ=255)
                                                                                                        ccGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAggag                                                                                                             >  2:1075084/1‑43 (MQ=255)
                                                                                                        ccGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAggag                                                                                                             <  1:1075084/45‑3 (MQ=255)
                                                                                                         cGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAggg                                                                                                              >  2:1340043/1‑43 (MQ=255)
                                                                                                         cGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAggg                                                                                                              <  1:1340043/43‑1 (MQ=255)
                                                                                                           ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                                >  2:1355137/1‑87 (MQ=255)
                                                                                                           ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                                <  1:1355137/87‑1 (MQ=255)
                                                                                                           ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCt            >  2:1647541/1‑139 (MQ=255)
                                                                                                           ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCt            >  2:515338/1‑139 (MQ=255)
                                                                                                           ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCt            >  1:793601/1‑139 (MQ=255)
                                                                                                            gCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATg                                                                                                      >  2:1583158/1‑48 (MQ=255)
                                                                                                            gCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATg                                                                                                      <  1:1583158/48‑1 (MQ=255)
                                                                                                             ccAGCACGGCACTGGTAATAATTGCTTAACGAAAc                                                                                                                  >  2:46485/1‑35 (MQ=255)
                                                                                                             ccAGCACGGCACTGGTAATAATTGCTTAACGAAAc                                                                                                                  <  1:46485/35‑1 (MQ=255)
                                                                                                                 cACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTggcggc      >  1:1314001/1‑139 (MQ=255)
                                                                                                                     gCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATg  <  1:699032/139‑1 (MQ=255)
                                                                                                                      cACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATg                                                                                                      <  2:352032/38‑1 (MQ=255)
                                                                                                                      cACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATg                                                                                                      >  1:352032/1‑38 (MQ=255)
                                                                                                                      cACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAAt                                                    <  1:943202/88‑1 (MQ=255)
                                                                                                                      cACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAAt                                                    >  2:943202/1‑88 (MQ=255)
                                                                                                                              |                                                                                                                                 
CCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATG  >  NC_000913/983497‑983752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: