Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,095,484	C→T	intergenic (‑47/‑21)	insE1 ← / → ymdE	IS3 transposase A/pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,095,484	0	C	T	100.0%	214.3 / NA	67	intergenic (‑47/‑21)	insE1/ymdE	IS3 transposase A/pseudogene
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/29);  total (38/29)

AGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACAGCAAAA  >  NC_000913/1095367‑1095615                                                                                                                      |                                                                                                                                    aGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTg                                                                                                                <  2:449315/139‑1 (MQ=14) aGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTg                                                                                                                >  2:913606/1‑139 (MQ=14)      ttCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTgg                                                                                                           <  2:472866/139‑1 (MQ=25)      ttCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTgg                                                                                                           >  2:613456/1‑139 (MQ=25)       tCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGc                                                                                                          >  2:993060/1‑139 (MQ=37)        cACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGca                                                                                                         >  1:550214/1‑139 (MQ=37)             cGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACAcgc                                                                                                    >  1:541254/1‑139 (MQ=255)                     aGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATg                                                                                            <  2:541254/139‑1 (MQ=255)                            tGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACg                                                                                     <  1:913606/139‑1 (MQ=255)                                  ttACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTc                                                                               <  1:440331/139‑1 (MQ=255)                                        ggTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCaa                                                                         <  1:613456/139‑1 (MQ=255)                                         gTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAAt                                                                        <  2:1500844/139‑1 (MQ=255)                                          ttttttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATg                                                                       <  1:1778708/139‑1 (MQ=255)                                                  tGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTa                                                               >  2:1520174/1‑139 (MQ=255)                                                   ggTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGtt                                                                                                 <  1:1711428/104‑1 (MQ=255)                                                   ggTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGtt                                                                                                 >  2:1711428/1‑104 (MQ=255)                                                   ggTTGATACTGTTTTTGGCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTAt                                                              >  2:1123941/1‑139 (MQ=255)                                                    gTTGATCCTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGcc                                                                                                              <  1:1386675/90‑1 (MQ=255)                                                    gTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGcc                                                                                                              >  2:1386675/1‑90 (MQ=25)                                                    gTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATc                                                             >  1:507026/1‑139 (MQ=255)                                                       gATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTg                                                          <  2:1435832/139‑1 (MQ=255)                                                            tGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGa                                                     <  2:1331819/139‑1 (MQ=255)                                                              tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGcaca                                                                                                       >  1:280776/1‑87 (MQ=255)                                                              tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGcaca                                                                                                       <  2:280776/87‑1 (MQ=255)                                                              tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACt                                                   >  2:944591/1‑139 (MQ=255)                                                              tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACt                                                   >  2:91174/1‑139 (MQ=255)                                                              tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACt                                                   >  1:1576369/1‑139 (MQ=255)                                                              tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACt                                                   >  2:1776803/1‑139 (MQ=255)                                                              tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACt                                                   >  1:1737407/1‑139 (MQ=255)                                                                  tGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGat                                               <  1:944591/139‑1 (MQ=255)                                                                   gTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGata                                              >  1:855508/1‑139 (MQ=255)                                                                   gTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGata                                              >  1:291980/1‑139 (MQ=255)                                                                       tgtgAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGc                                          <  1:91174/139‑1 (MQ=255)                                                                        gtgAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGcac                                                                                                        >  2:1714742/1‑76 (MQ=255)                                                                        gtgAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGcac                                                                                                        <  1:1714742/76‑1 (MQ=255)                                                                        gtgAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGtt                                                                                <  2:389241/100‑1 (MQ=255)                                                                        gtgAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGtt                                                                                >  1:389241/1‑100 (MQ=255)                                                                         tgAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATg                                                                       >  1:1325419/1‑108 (MQ=255)                                                                         tgAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATg                                                                       <  2:1325419/108‑1 (MQ=255)                                                                             tCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGcc                                    <  1:1123941/139‑1 (MQ=255)                                                                              cACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCa                                   <  2:1576369/139‑1 (MQ=255)                                                                              cACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCa                                   >  1:833103/1‑139 (MQ=255)                                                                                ccTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCaga                                 <  2:507026/139‑1 (MQ=255)                                                                                    tgactgaGAGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCAt                             <  2:291980/139‑1 (MQ=255)                                                                                         gagaGTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGt                        <  1:993060/139‑1 (MQ=255)                                                                                          agagTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGtt                       >  1:1370336/1‑139 (MQ=255)                                                                                          agagTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGtt                       >  2:710051/1‑139 (MQ=255)                                                                                          agagTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGtt                       >  1:802359/1‑139 (MQ=255)                                                                                          agagTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGtt                       >  2:413283/1‑139 (MQ=255)                                                                                           gagTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTc                      >  1:466360/1‑139 (MQ=255)                                                                                           gagTTTACTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTc                      >  1:503053/1‑139 (MQ=255)                                                                                                  cTCACTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGaa               <  2:833103/139‑1 (MQ=255)                                                                                                      cTTAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTa           >  2:1657741/1‑139 (MQ=255)                                                                                                        tAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTc                                                                               >  1:236691/1‑69 (MQ=255)                                                                                                        tAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTc                                                                               <  2:236691/69‑1 (MQ=255)                                                                                                        tAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGat                                               <  1:1108726/101‑1 (MQ=255)                                                                                                        tAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGtt                       <  2:17859/125‑1 (MQ=255)                                                                                                        tAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGtt                       >  1:17859/1‑125 (MQ=255)                                                                                                        tAGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGAGCGATTATCTTGCCGGACTGGat                                               >  2:1108726/1‑101 (MQ=255)                                                                                                         aGCCGCGTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACa        >  2:1490802/1‑139 (MQ=255)                                                                                                             gcgTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACAGCaa    >  1:828172/1‑139 (MQ=255)                                                                                                             gcgTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACAGCaa    >  2:1189263/1‑139 (MQ=255)                                                                                                             gcgTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACAGCa     <  2:1640707/138‑1 (MQ=255)                                                                                                             gcgTGTCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACAGCa     >  1:1640707/1‑138 (MQ=255)                                                                                                               gtgtCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACAGCaaaa  >  1:1463349/1‑139 (MQ=255)                                                                                                                tgtCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACTGCGTTCAGGCAATGCGCGATTATCTTGCCTGACTGGATATCGCTTCGCCAGAGCATc                            >  1:1183316/1‑112 (MQ=255)                                                                                                                tgtCCATTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATc                            <  2:1183316/112‑1 (MQ=255)                                                                                                                      |                                                                                                                                    AGGGCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGTGCCTGGCACACGCGTTTGATGGAAGACGGCGTTCAGGCAATGCGCGATTATCTTGCCGGACTGGATATCGCTTCGCCAGAGCATCAGGTTCTGATGAACGTAACAGCAAAA  >  NC_000913/1095367‑1095615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑