Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,307	C→T	*47* (TAG→TAA)	yceO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,307	0	C	T	100.0%	141.2 / NA	45	*47* (TAG→TAA)	yceO	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/21);  total (24/21)

AATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGC  >  NC_000913/1119172‑1119442                                                                                                                                        |                                                                                                                                          aaTCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaa                                                                                                                                        >  1:430197/1‑139 (MQ=255)    cTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTaaaaa                                                                                                                                     >  1:1788371/1‑139 (MQ=255)        aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaaa                                                                                                                                 <  2:430197/139‑1 (MQ=255)                  tGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATagat                                                                                                                     <  1:1242121/139‑1 (MQ=255)                  tGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATagat                                                                                                                     >  2:1339509/1‑139 (MQ=255)                       tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATa                                                                                                                                   <  1:10859/122‑1 (MQ=255)                       tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATa                                                                                                                                   >  2:10859/1‑122 (MQ=255)                       tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑Acaca                                                                                                                          >  1:1022452/1‑129 (MQ=255)                       tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑Acaca                                                                                                                          <  2:1022452/129‑1 (MQ=255)                        cAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATagatcagatc                                                                                                               >  1:829143/1‑139 (MQ=255)                                 agaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGc                                                                                                      <  1:1339509/139‑1 (MQ=255)                                 agaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGc                                                                                                      <  2:522397/139‑1 (MQ=255)                                   aTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCca                                                                                                    >  2:778300/1‑139 (MQ=255)                                     gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAAACACACATAGATCAGATCCATAATTGCca                                                                                                    >  2:1308066/1‑139 (MQ=255)                                     gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCcaac                                                                                                  >  2:278937/1‑139 (MQ=255)                                     gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCcaac                                                                                                  >  1:588174/1‑139 (MQ=255)                                             aGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaa                                                                                                                                  <  1:940294/101‑1 (MQ=255)                                             aGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATaa                                                                                                                                  >  2:940294/1‑101 (MQ=255)                                               tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCa                                                                                          <  1:456968/137‑1 (MQ=255)                                               tGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCa                                                                                          >  2:456968/1‑137 (MQ=255)                                                   gTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGaaa                                                                                    <  2:1788371/139‑1 (MQ=255)                                                              aataatCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATagatcag                                                                                                                  <  2:1420955/98‑1 (MQ=255)                                                              aataatCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATagatcag                                                                                                                  >  1:1420955/1‑98 (MQ=255)                                                                   tctcATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGa                                                                    >  1:255564/1‑139 (MQ=255)                                                                   tctcATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGa                                                                    >  2:1485604/1‑139 (MQ=255)                                                                     tcATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATg                                                                  <  1:278937/139‑1 (MQ=255)                                                                             tttttCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                          <  1:498638/139‑1 (MQ=255)                                                                                     gCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATtg                                                  <  2:588174/139‑1 (MQ=255)                                                                                               aGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAAtatt                                                      <  1:446874/125‑1 (MQ=255)                                                                                               aGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGAATCAGATGCTCGCGAAtatt                                                      >  2:446874/1‑125 (MQ=255)                                                                                                   agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑acac                                                                                                                           >  2:1275287/1‑52 (MQ=255)                                                                                                   agaTTAAATATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑acac                                                                                                                           <  1:1275287/52‑1 (MQ=255)                                                                                                         aaTATTTGTCAGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCg                              <  2:255564/139‑1 (MQ=255)                                                                                                                   aGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCCGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgc                                                             >  2:1203922/1‑98 (MQ=255)                                                                                                                   aGTTTATGTCAGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTcgc                                                             <  1:1203922/98‑1 (MQ=255)                                                                                                                             aGTGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTa          >  1:1039809/1‑139 (MQ=255)                                                                                                                               tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATa                                                                              <  1:1414568/69‑1 (MQ=255)                                                                                                                               tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATa                                                                              >  2:1414568/1‑69 (MQ=255)                                                                                                                               tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                          >  2:1518580/1‑89 (MQ=255)                                                                                                                               tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGaa                                                          <  1:1518580/89‑1 (MQ=255)                                                                                                                               tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTaaa                    <  2:1611370/127‑1 (MQ=255)                                                                                                                               tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTaaa                    >  1:1611370/1‑127 (MQ=255)                                                                                                                               tGCGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaa        >  2:1548894/1‑139 (MQ=255)                                                                                                                                 cGGAAAATTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTaaaaa      >  1:1785156/1‑139 (MQ=255)                                                                                                                                     aaaTTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGc  <  2:1785156/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                          AATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATATTTGTCAGTTTATGTCAGTGCGGAAAACTAAAAATAAA‑‑ACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGC  >  NC_000913/1119172‑1119442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑