Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	115,661	T→C	M22V (ATG→GTG)	hofC ←	assembly protein in type IV pilin biogenesis, transmembrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	115,661	0	T	C	100.0%	201.8 / NA	57	M22V (ATG→GTG)	hofC	assembly protein in type IV pilin biogenesis, transmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/27);  total (30/27)

ACTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGTTTT  >  NC_000913/115537‑115796                                                                                                                             |                                                                                                                                        aCTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCAt                                                                                                                           >  2:1419869/1‑139 (MQ=255)         gcTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                                   <  2:475653/139‑1 (MQ=255)                    ctcGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgcc                                                                                                        <  2:503363/139‑1 (MQ=255)                       gCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCtg                                                                                                                                 >  2:1079158/1‑110 (MQ=255)                       gCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatg                                                                                                     >  2:894806/1‑139 (MQ=255)                       gCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatg                                                                                                     >  1:1515842/1‑139 (MQ=255)                       gCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCGCTGCCCATAGCACCCCATCtt                                                                                                                                 <  1:1079158/111‑1 (MQ=255)                            tGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAgc                                                                                                <  2:831569/139‑1 (MQ=255)                              cgcAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGGCATGCCAgcgc                                                                                              >  2:1168662/1‑139 (MQ=255)                                cAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCa                                                                                            <  1:1376063/139‑1 (MQ=255)                                 aGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCag                                                                                           >  1:1460784/1‑139 (MQ=255)                                        aTGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATTCCATGCCAGCGCCAGAGTTGCt                                                                                    >  2:1775257/1‑139 (MQ=255)                                             gATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTc                                                                               >  2:1462701/1‑139 (MQ=255)                                                       aGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGc                                                                     <  1:1419869/139‑1 (MQ=255)                                                       aGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGc                                                                     <  1:1462701/139‑1 (MQ=255)                                                                 gggTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTa                                                           <  1:1168662/139‑1 (MQ=255)                                                                         aTCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGAtt                                                   >  1:153790/1‑139 (MQ=255)                                                                         aTCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGAtt                                                   >  1:813588/1‑139 (MQ=255)                                                                         aTCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGGCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGAtt                                                   <  2:1791998/139‑1 (MQ=255)                                                                           cTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                                 <  2:1460784/139‑1 (MQ=255)                                                                           cTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                                 <  1:894806/139‑1 (MQ=255)                                                                                     aGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAg                                       <  2:1515842/139‑1 (MQ=255)                                                                                      gTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATccc                                                                >  1:252961/1‑113 (MQ=255)                                                                                      gTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATccc                                                                <  2:252961/113‑1 (MQ=255)                                                                                         ccATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAggtgg                                   >  1:496565/1‑139 (MQ=255)                                                                                           aTAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGtt                                 <  2:496565/139‑1 (MQ=255)                                                                                              aGCAGTAATGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                                 >  2:1169717/1‑120 (MQ=255)                                                                                              aGCAGTAAAGTACGGCTCTCTGCCGATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                                 <  1:1169717/120‑1 (MQ=255)                                                                                                   tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCAt                                                                          <  2:1525894/90‑1 (MQ=255)                                                                                                   tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCAt                                                                          >  1:1525894/1‑90 (MQ=255)                                                                                                   tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcg                                                         <  2:1209527/107‑1 (MQ=255)                                                                                                   tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcg                                                         >  1:1209527/1‑107 (MQ=255)                                                                                                   tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                                 >  2:1145499/1‑115 (MQ=255)                                                                                                   tAAAGTACGGCTCGCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                                 <  1:1145499/115‑1 (MQ=255)                                                                                                      aGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGctc                      >  2:19445/1‑139 (MQ=255)                                                                                                        tACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCTTTGCTCTa                    >  1:1002334/1‑139 (MQ=255)                                                                                                           ggCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGc                                                                        <  1:335753/84‑1 (MQ=255)                                                                                                           ggCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGc                                                                        >  2:335753/1‑84 (MQ=255)                                                                                                             ctctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                              >  2:1757202/1‑44 (MQ=255)                                                                                                             ctctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                              <  1:1757202/44‑1 (MQ=255)                                                                                                             ctctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCagag                                                                                         >  1:639891/1‑65 (MQ=255)                                                                                                             ctctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCagag                                                                                         <  2:639891/65‑1 (MQ=255)                                                                                                             ctctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATccc                                                                <  2:1582397/90‑1 (MQ=255)                                                                                                             ctctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATccc                                                                >  1:1582397/1‑90 (MQ=255)                                                                                                               ctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTg                         <  1:881449/127‑1 (MQ=255)                                                                                                               ctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTg                         >  2:881449/1‑127 (MQ=255)                                                                                                                tctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                                 >  1:371216/1‑102 (MQ=255)                                                                                                                tctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                                 <  2:371216/102‑1 (MQ=255)                                                                                                                  tGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGc                                                                     <  2:1192040/80‑1 (MQ=255)                                                                                                                  tGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGc                                                                     >  1:1192040/1‑80 (MQ=255)                                                                                                                  tGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGAt                                                    <  1:1221536/97‑1 (MQ=255)                                                                                                                  tGCCCATAGCACCCCATCTTACGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGAt                                                    >  2:1221536/1‑97 (MQ=255)                                                                                                                         aGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAg                                       <  1:1410411/103‑1 (MQ=255)                                                                                                                         aGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAg                                       >  2:1410411/1‑103 (MQ=255)                                                                                                                         aGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGttt   >  1:129986/1‑139 (MQ=255)                                                                                                                         aGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGttt   >  1:1654225/1‑139 (MQ=255)                                                                                                                          gCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGtttt  >  2:828863/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                        ACTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGTTTT  >  NC_000913/115537‑115796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑