Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,862	A→G	D11G (GAC→GGC)	acnA →	aconitate hydratase 1, aconitase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,862	0	A	G	100.0%	310.2 / NA	87	D11G (GAC→GGC)	acnA	aconitate hydratase 1, aconitase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/38);  total (49/38)

GGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGT  >  NC_000913/1335735‑1335995                                                                                                                                |                                                                                                                                      gggTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAgg                                                                                                                            >  2:251232/1‑139 (MQ=255)          tCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATa                                                                                                                   <  1:23348/139‑1 (MQ=255)                 gTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTa                                                                                                            >  2:1176738/1‑139 (MQ=255)                 gTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTa                                                                                                            >  2:1502528/1‑139 (MQ=255)                    aCGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACg                                                                                                                                   <  2:861252/113‑1 (MQ=255)                    aCGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACg                                                                                                                                   >  1:861252/1‑113 (MQ=255)                    aCGCGATGTTTGTGTTATCATTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCCATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAATCTTACCa                                                                                                         <  1:1176738/139‑1 (MQ=255)                          tGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCActacta                                                                                                   >  1:1301036/1‑139 (MQ=255)                          tGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCATGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCActacta                                                                                                   >  2:1181239/1‑139 (MQ=255)                                    aTCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAcc                                                                                                          <  1:1262059/122‑1 (MQ=255)                                    aTCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAcc                                                                                                          >  2:1262059/1‑122 (MQ=255)                                    aTCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTATCACTACTACAGCCTGCCg                                                                                         >  2:1210002/1‑139 (MQ=255)                                         tAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTtgc                                                                                    <  1:1502528/139‑1 (MQ=255)                                               tCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCa                                                                                                         <  2:71695/112‑1 (MQ=255)                                               tCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCa                                                                                                         >  1:71695/1‑112 (MQ=255)                                                cACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTCGCTGCTaaa                                                                             >  1:1162124/1‑139 (MQ=255)                                                   ccTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACtt                                                                                                             <  2:471709/104‑1 (MQ=255)                                                   ccTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACtt                                                                                                             >  1:471709/1‑104 (MQ=255)                                                   acTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCa                                                                          >  1:1122774/2‑139 (MQ=255)                                                      gAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAc                                                                                                           >  2:421433/1‑103 (MQ=255)                                                      gAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCGACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAc                                                                                                           <  1:421433/103‑1 (MQ=255)                                                       aaGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTgg                                                                      >  1:953343/1‑139 (MQ=255)                                                       aaGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTgg                                                                      <  1:137575/139‑1 (MQ=255)                                                            aaTCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACt                                                                                                              >  2:298566/1‑94 (MQ=255)                                                            aaTCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACt                                                                                                              <  1:298566/94‑1 (MQ=255)                                                            aaTCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGat                                                                 >  2:475267/1‑139 (MQ=255)                                                             aTCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGc                                                                                               >  1:1140115/1‑108 (MQ=255)                                                             aTCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGata                                                                >  1:848380/1‑139 (MQ=255)                                                             aTCAGGGCTTCGCAACCCTCTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGc                                                                                               <  2:1140115/108‑1 (MQ=255)                                                             aTCAGGGCTTCGCAACACTGTCATTAAGGAGGAGCTATGTCGTCAACACTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAACACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGCGCGata                                                                >  2:553650/1‑139 (MQ=255)                                                               cAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATc                                                              <  1:1181239/139‑1 (MQ=255)                                                                aGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACAAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCa                                                             <  2:953343/139‑1 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGc                                                                                                                               >  2:783368/1‑72 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGc                                                                                                                               <  1:783368/72‑1 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACtt                                                                                                             >  2:392501/1‑90 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACtt                                                                                                             <  1:392501/90‑1 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACtt                                                                                                             >  2:1240764/1‑90 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACtt                                                                                                             <  1:1240764/90‑1 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCa                                                             <  2:1694712/138‑1 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCa                                                             >  1:1694712/1‑138 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCAc                                                            >  2:1793085/1‑139 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCAc                                                            >  2:143661/1‑139 (MQ=255)                                                                 gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCAc                                                            >  1:226097/1‑139 (MQ=255)                                                                     ttCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGt                                                        <  1:1210002/139‑1 (MQ=255)                                                                     ttCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGt                                                        <  1:143661/139‑1 (MQ=255)                                                                      tCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTc                                                       >  2:1249009/1‑139 (MQ=255)                                                                       cGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAc                                                                                                           >  2:1731591/1‑86 (MQ=255)                                                                       cGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAc                                                                                                           <  1:1731591/86‑1 (MQ=255)                                                                       cGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCt                                                      >  2:100319/1‑139 (MQ=255)                                                                         cAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGACGATATCACCCGTCTAc                                                    <  2:1122774/139‑1 (MQ=255)                                                                               tGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAcc                                                                                                          <  2:264068/79‑1 (MQ=255)                                                                               tGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAcc                                                                                                          >  1:264068/1‑79 (MQ=255)                                                                               tGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGt                                                        >  2:1327330/1‑129 (MQ=255)                                                                               tGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGt                                                        <  1:1327330/129‑1 (MQ=255)                                                                                 tCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTgg                                                                      >  1:861804/1‑113 (MQ=255)                                                                                 tCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTgg                                                                      <  2:861804/113‑1 (MQ=255)                                                                                 tCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCa                                            >  2:1123347/1‑139 (MQ=255)                                                                                   aTTAAGGAAGAGCTATGTCGTCAACCCTACCAAAAGCCAGTAAGGGCACGTTGCAAGCCAAAGATAAAACTTACCACTACCTCCGCCTGCCGCTTGGCGGTTAATCACTGGGCGATATCACCCGTCTACCCAAGTCACt                                          <  2:1301036/139‑1 (MQ=255)                                                                                     tAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCa                                        <  1:475267/139‑1 (MQ=255)                                                                                      aaGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCaa                                       >  1:295304/1‑139 (MQ=255)                                                                                          aggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGtt                                   >  1:165211/1‑139 (MQ=255)                                                                                           ggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAGAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGttt                                  >  1:371033/1‑139 (MQ=255)                                                                                           ggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGttt                                  >  1:877583/1‑139 (MQ=255)                                                                                             agCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTg                                >  2:1452900/1‑139 (MQ=255)                                                                                             agCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTg                                >  1:863584/1‑139 (MQ=255)                                                                                               cTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCt                              <  2:848380/139‑1 (MQ=255)                                                                                                 aTGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCg                            <  1:1793085/139‑1 (MQ=255)                                                                                                   gtcgtcAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTgg                                                                      >  2:985750/1‑95 (MQ=255)                                                                                                   gtcgtcAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTgg                                                                      <  1:985750/95‑1 (MQ=255)                                                                                                    tcgtcAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCg                                                         >  1:1144218/1‑107 (MQ=255)                                                                                                    tcgtcAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCg                                                         <  2:1144218/107‑1 (MQ=255)                                                                                                      gtcAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATa                                                                                                                   <  2:988097/47‑1 (MQ=255)                                                                                                      gtcAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATa                                                                                                                   >  1:988097/1‑47 (MQ=255)                                                                                                      gtcAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAAc                       >  2:163966/1‑139 (MQ=255)                                                                                                       tcAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGcc                                                                                                                          <  1:1467414/39‑1 (MQ=255)                                                                                                       tcAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGcc                                                                                                                          >  2:1467414/1‑39 (MQ=255)                                                                                                           cccTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACctgct                  <  1:100319/139‑1 (MQ=255)                                                                                                               aCGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTgcgc              >  1:1713297/1‑139 (MQ=255)                                                                                                                  agaAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTgg           <  2:877583/139‑1 (MQ=255)                                                                                                                     aGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAAt                                                                            >  2:380241/1‑71 (MQ=255)                                                                                                                     aGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAAt                                                                            <  1:380241/71‑1 (MQ=255)                                                                                                                     aGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAg        >  1:1620701/1‑139 (MQ=255)                                                                                                                         aGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCGGGATg    >  1:383398/1‑139 (MQ=255)                                                                                                                         aGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAg        <  1:1587415/135‑1 (MQ=255)                                                                                                                         aGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAg        >  2:1587415/1‑135 (MQ=255)                                                                                                                           tAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATgga  <  1:1123347/139‑2 (MQ=255)                                                                                                                           tAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGt  <  2:165211/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                      GGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGT  >  NC_000913/1335735‑1335995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑