Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,288	G→A	F77F (TTC→TTT)	sapC ←	antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,288	0	G	A	100.0%	115.9 / NA	37	F77F (TTC→TTT)	sapC	antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

GGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGG  >  NC_000913/1354179‑1354423                                                                                                              |                                                                                                                                        ggCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAgc                                                                                                                   <  2:996589/132‑1 (MQ=255) ggCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAgc                                                                                                                   >  1:996589/1‑132 (MQ=255) ggCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCa                                                                                                            >  1:266965/1‑139 (MQ=255)    cacaGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGa                                                                                                         >  1:336801/1‑139 (MQ=255)      caGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAgaaagaaa                                                                                                                                   >  2:296296/1‑111 (MQ=255)      caGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAgaaagaaa                                                                                                                                   <  1:296296/111‑1 (MQ=255)      caGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGAcg                                                                                                       >  2:985625/1‑139 (MQ=255)             tcgCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaa                                                                                                <  1:985625/139‑1 (MQ=255)             tcgCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaa                                                                                                <  1:306668/139‑1 (MQ=255)             tcgCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaa                                                                                                <  1:1189982/139‑1 (MQ=255)                       cGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGAcggc                                                                                                     >  2:918964/1‑124 (MQ=255)                       cGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGAcggc                                                                                                     <  1:918964/124‑1 (MQ=255)                       cGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATaa                                                                                      <  2:266965/139‑1 (MQ=255)                                       ccacccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGt                                                                      >  1:109560/1‑139 (MQ=255)                                          cccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGa                                                                   <  1:879070/139‑1 (MQ=255)                                                   ggCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGt                                                                      <  1:303532/127‑1 (MQ=255)                                                   ggCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGt                                                                      >  2:303532/1‑127 (MQ=255)                                                    gcgcAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAgc                                                                                                                   >  1:1343881/1‑81 (MQ=255)                                                    gcgcAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAgc                                                                                                                   <  2:1343881/81‑1 (MQ=255)                                                    gcgcAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAg                                                          <  1:1078888/138‑1 (MQ=255)                                                    gcgcAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAg                                                          >  2:1078888/1‑138 (MQ=255)                                                    gcgcAGCTCCGCTCAGTAAACGACTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAgg                                                         >  1:374196/1‑139 (MQ=255)                                                     cgcAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGgc                                                        >  2:363122/1‑139 (MQ=255)                                                          cTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCaaa                                                   >  1:712177/1‑139 (MQ=255)                                                             cGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGt                                                                      >  1:1745098/1‑117 (MQ=255)                                                             cGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGt                                                                      <  2:1745098/117‑1 (MQ=255)                                                               cTCAGTAAGCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAACCCAgc                                              >  1:1299453/1‑139 (MQ=255)                                                               cTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAAc                                                                                     <  2:1268694/100‑1 (MQ=255)                                                               cTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCAAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAAc                                                                                     >  1:1268694/1‑100 (MQ=255)                                                                           cTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATac                                  <  2:374196/139‑1 (MQ=255)                                                                                aaCATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaa                                                                                                >  1:686622/1‑72 (MQ=255)                                                                                aaCATCGCGTCCGAGGTCGTCAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaa                                                                                                <  2:686622/72‑1 (MQ=255)                                                                                               gtcgtcAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCcgcg              >  2:573482/1‑139 (MQ=255)                                                                                                 cgtcAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCa            <  2:109560/139‑1 (MQ=255)                                                                                                    cAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCg                                                                    <  2:886602/80‑1 (MQ=255)                                                                                                    cAGTCCCCAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCg                                                                    >  1:886602/1‑80 (MQ=255)                                                                                                         cccAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAgg                                                         >  2:1535574/1‑86 (MQ=255)                                                                                                         cccAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAgg                                                         <  1:1535574/86‑1 (MQ=255)                                                                                                           cAGAAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAgg  <  2:336801/139‑1 (MQ=255)                                                                                                              |                                                                                                                                        GGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGG  >  NC_000913/1354179‑1354423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑