Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,547,061	C→T	A37T (GCA→ACA)	yddG ←	aromatic amino acid exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,547,061	0	C	T	100.0%	192.7 / NA	59	A37T (GCA→ACA)	yddG	aromatic amino acid exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/30);  total (29/30)

AGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTT  >  NC_000913/1546928‑1547196                                                                                                                                      |                                                                                                                                        aGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGtgccgc                                                                                                                                    >  2:839203/1‑139 (MQ=255)  gcgcCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTgccgcc                                                                                                                                   >  2:554774/1‑139 (MQ=255)           agaTTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGcc                                                                                                                          <  1:1690073/139‑1 (MQ=255)              tttCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAg                                                                                                                       <  2:833742/139‑1 (MQ=255)               ttCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCTGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGc                                                                                                                      <  2:621778/139‑1 (MQ=255)                                   gACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaat                                                                                                  <  1:279453/139‑1 (MQ=255)                                   gACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaat                                                                                                  >  1:1527124/1‑139 (MQ=255)                                    aCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaatc                                                                                                 <  1:554774/139‑1 (MQ=255)                                      tCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGAcc                                                                                                                               <  2:1239646/107‑1 (MQ=255)                                      tCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGAcc                                                                                                                               >  1:1239646/1‑107 (MQ=255)                                      tCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaatcaa                                                                                               <  1:839203/139‑1 (MQ=255)                                      tCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaatcaa                                                                                               <  2:147832/139‑1 (MQ=255)                                              aGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCa                                                                                       <  2:1527124/139‑1 (MQ=255)                                                tAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATc                                                                                     >  1:831981/1‑139 (MQ=255)                                                  aaTAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGt                                                                                   >  2:231594/1‑139 (MQ=255)                                                   aTAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTg                                                                                  <  2:1612397/139‑1 (MQ=255)                                                   aTAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTg                                                                                  <  1:795000/139‑1 (MQ=255)                                                          ttCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaat                                                                                                  >  2:1526610/1‑116 (MQ=255)                                                          ttCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaat                                                                                                  <  1:1526610/116‑1 (MQ=255)                                                          ttCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTg                                                                                  <  1:426003/132‑1 (MQ=255)                                                          ttCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTg                                                                                  >  2:426003/1‑132 (MQ=255)                                                          ttCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCcaca                                                                           >  2:1115396/1‑139 (MQ=255)                                                                               ggAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTc                                                                               >  1:1673760/1‑114 (MQ=255)                                                                               ggAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTc                                                                               <  2:1673760/114‑1 (MQ=255)                                                                                     ccAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCg                                                <  1:231594/139‑1 (MQ=255)                                                                                              aaGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCttttt                                       >  1:1574213/1‑139 (MQ=255)                                                                                               aGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg                                                           <  1:873750/118‑1 (MQ=255)                                                                                               aGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg                                                           >  2:873750/1‑118 (MQ=255)                                                                                                   tAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCATTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg                                                           >  2:609535/1‑114 (MQ=255)                                                                                                   tAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg                                                           <  1:609535/114‑1 (MQ=255)                                                                                                        gcagcCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCAt                             >  2:1326436/1‑139 (MQ=255)                                                                                                         cagcCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATg                            >  1:1525232/1‑139 (MQ=255)                                                                                                          agcCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGc                           <  1:1326436/139‑1 (MQ=255)                                                                                                            cccGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCg                                                                                                                              >  2:97934/1‑38 (MQ=255)                                                                                                            cccGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCg                                                                                                                              <  1:97934/38‑1 (MQ=255)                                                                                                                cTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg                                                           <  2:1394705/101‑1 (MQ=255)                                                                                                                cTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg                                                           >  1:1394705/1‑101 (MQ=255)                                                                                                                         tagatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCg                        <  1:727466/127‑1 (MQ=255)                                                                                                                         tagatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCg                        >  2:727466/1‑127 (MQ=255)                                                                                                                         tagatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGtctc            >  1:1502409/1‑139 (MQ=255)                                                                                                                          agatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaa                                                                                                   >  2:1033718/1‑51 (MQ=255)                                                                                                                          agatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaa                                                                                                   <  1:1033718/51‑1 (MQ=255)                                                                                                                           gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTc                                                                               <  1:877597/70‑1 (MQ=255)                                                                                                                           gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTc                                                                               >  2:877597/1‑70 (MQ=255)                                                                                                                           gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGa                                                                     >  1:955050/1‑80 (MQ=255)                                                                                                                           gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGa                                                                     <  2:955050/80‑1 (MQ=255)                                                                                                                           gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCg                                                <  2:1074432/101‑1 (MQ=255)                                                                                                                           gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCg                                                >  1:1074432/1‑101 (MQ=255)                                                                                                                           gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGtctctc          >  1:1184931/1‑139 (MQ=255)                                                                                                                            atagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTctctct         <  2:1184931/139‑1 (MQ=255)                                                                                                                              agcagcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTa                                                                                          <  1:1751547/56‑1 (MQ=255)                                                                                                                              agcagcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTa                                                                                          >  2:1751547/1‑56 (MQ=255)                                                                                                                                cagcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGa                                                                     >  2:890031/1‑75 (MQ=255)                                                                                                                                cagcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGa                                                                     <  1:890031/75‑1 (MQ=255)                                                                                                                                 agcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGa                                                                        >  2:662041/1‑71 (MQ=255)                                                                                                                                 agcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGa                                                                        <  1:662041/71‑1 (MQ=255)                                                                                                                                 agcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGt               <  1:1735967/128‑1 (MQ=255)                                                                                                                                 agcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGt               >  2:1735967/1‑128 (MQ=255)                                                                                                                                 agcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCg    >  2:351026/1‑139 (MQ=255)                                                                                                                                   cTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGtt  >  1:1599210/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        AGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTT  >  NC_000913/1546928‑1547196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑