Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,726,622 G→A *200* (TAG→TAA nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,726,6220GA100.0% 100.3 / NA 32*200* (TAG→TAAnemRtranscriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)

AACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTGCGCAGTA  >  NC_000913/1726486‑1726752
                                                                                                                                        |                                                                                                                                  
aaCCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACa                                                                                                                                  <  1:1074807/139‑1 (MQ=255)
                acaAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCAcc                                                                                                                  >  1:1257520/1‑139 (MQ=255)
                         ttACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGC‑‑CTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCggg                                                                                                       <  1:621247/139‑1 (MQ=255)
                            cGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGa                                                                                                      <  2:131750/139‑1 (MQ=255)
                                 tgtgGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCtt                                                                                                 >  1:407029/1‑139 (MQ=255)
                                  gtgGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCttt                                                                                                >  2:937336/1‑139 (MQ=255)
                                    ggCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTAt                                                                                              <  2:1257520/139‑1 (MQ=255)
                                                                  cgcgCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGaa                                                                >  1:1734901/1‑139 (MQ=255)
                                                                                       aaaaCGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACggcggc                                           <  2:407029/139‑1 (MQ=255)
                                                                                              gcTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGt                                    <  2:1734901/139‑1 (MQ=255)
                                                                                                      aTGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTAt                            >  1:524613/1‑139 (MQ=255)
                                                                                                            aaaaCATTATTGCGACGCCTGCCGTTTAACAGGCa                                                                                                                              <  2:205943/35‑1 (MQ=255)
                                                                                                            aaaaCATTATTGCGACGCCTGCCGTTTAACAGGCa                                                                                                                              >  1:205943/1‑35 (MQ=255)
                                                                                                            aaaaCATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCaaa                                        <  2:52638/121‑1 (MQ=255)
                                                                                                            aaaaCATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCaaa                                        >  1:52638/1‑121 (MQ=255)
                                                                                                            aaaaCATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATgg                          >  1:1601932/1‑135 (MQ=255)
                                                                                                            aaaaCATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATgg                          <  2:1601932/135‑1 (MQ=255)
                                                                                                                  ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCgg                                                                                                        >  1:791966/1‑51 (MQ=255)
                                                                                                                  ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCgg                                                                                                        <  2:791966/51‑1 (MQ=255)
                                                                                                                  ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTccc                                                                       >  1:840396/1‑84 (MQ=255)
                                                                                                                  ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTccc                                                                       <  2:840396/84‑1 (MQ=255)
                                                                                                                  ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTccc                                                                       >  2:338825/1‑84 (MQ=255)
                                                                                                                  ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTccc                                                                       <  1:338825/84‑1 (MQ=255)
                                                                                                                   tattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAcg               <  1:937336/139‑1 (MQ=255)
                                                                                                                   tattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAcg               >  1:635946/1‑139 (MQ=255)
                                                                                                                        cGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACggcggc                                           >  1:43014/1‑106 (MQ=255)
                                                                                                                        cGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACggcggc                                           <  2:43014/106‑1 (MQ=255)
                                                                                                                          aCGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAg                                                              >  1:132644/1‑85 (MQ=255)
                                                                                                                          aCGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAg                                                              <  2:132644/85‑1 (MQ=255)
                                                                                                                               tGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCg                                     <  2:1575024/105‑1 (MQ=255)
                                                                                                                               tGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCg                                     >  1:1575024/1‑105 (MQ=255)
                                                                                                                                gCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTGCGCAGTa  <  2:635946/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                  
AACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTGCGCAGTA  >  NC_000913/1726486‑1726752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: