Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,761,518	G→A	R92C (CGT→TGT)	sufD ←	component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,761,518	0	G	A	100.0%	169.5 / NA	53	R92C (CGT→TGT)	sufD	component of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/28);  total (25/28)

GCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTT  >  NC_000913/1761384‑1761652                                                                                                                                       |                                                                                                                                           gCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCa                                                                                                                                        <  2:685674/139‑1 (MQ=255)             gCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACAcc                                                                                                                        >  1:792262/1‑139 (MQ=255)                 tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGcc                                                                                                                    >  1:1476090/1‑139 (MQ=255)                    gTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCa                                                                                                                 <  2:1033647/139‑1 (MQ=255)                     tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCAc                                                                                                                <  1:67416/139‑1 (MQ=255)                      cAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACg                                                                                                               <  2:104900/139‑1 (MQ=255)                      cAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACg                                                                                                               <  2:1476090/139‑1 (MQ=255)                            cAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCt                                                                                                         <  1:118862/139‑1 (MQ=255)                            cAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCt                                                                                                         <  2:517608/139‑1 (MQ=255)                                 acacTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGt                                                                                                    <  2:1687159/139‑1 (MQ=255)                                     ttCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAAc                                                                                                >  1:1514753/1‑139 (MQ=255)                                      tCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACg                                                                                               >  1:1389202/1‑139 (MQ=255)                                             gAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGc                                                                                        >  2:992458/1‑139 (MQ=255)                                               aTAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCAt                                                                                      >  1:41469/1‑139 (MQ=255)                                                   cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                  >  2:705912/1‑139 (MQ=255)                                                   cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                  <  2:1514753/139‑1 (MQ=255)                                                   cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                  <  1:705912/139‑1 (MQ=255)                                                        ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                                             <  1:417372/139‑1 (MQ=255)                                                        ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                                             >  1:904206/1‑139 (MQ=255)                                                          tAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCtgt                                                                           <  2:904206/139‑1 (MQ=255)                                                                  gACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGatat                                                                   >  2:228093/1‑139 (MQ=255)                                                                  gACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGatat                                                                   <  1:1207108/139‑1 (MQ=255)                                                                   aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtc                                                                  >  1:1467724/1‑139 (MQ=255)                                                                   aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtc                                                                  >  1:258490/1‑139 (MQ=255)                                                                      gtcgtcGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtctc                                                                <  1:180607/138‑1 (MQ=255)                                                                      gtcgtcGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtctc                                                                >  2:180607/1‑138 (MQ=255)                                                                         gtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGtt                                                                                                   <  2:363160/100‑1 (MQ=255)                                                                         gtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGtt                                                                                                   >  1:363160/1‑100 (MQ=255)                                                                            gtTAATGCTCACTTCATATCCTCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCa                                                         <  2:1056149/139‑1 (MQ=255)                                                                                       cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              <  2:1389202/139‑1 (MQ=255)                                                                                          cATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGTACGTAACACCCATCGACAAACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                  >  2:267410/1‑104 (MQ=255)                                                                                          cATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGTACGTAACACCCATCGACAAACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                  <  1:267410/104‑1 (MQ=255)                                                                                          cATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTg                                           >  2:1440082/1‑139 (MQ=255)                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              >  1:1270641/1‑128 (MQ=255)                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              >  1:905748/1‑128 (MQ=255)                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              <  2:905748/128‑1 (MQ=255)                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              <  2:1270641/128‑1 (MQ=255)                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGa                                   >  2:1451858/1‑139 (MQ=255)                                                                                                     ccTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCa                                >  2:87890/1‑139 (MQ=255)                                                                                                       ttCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGc                              <  2:1467724/139‑1 (MQ=255)                                                                                                          aGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              >  1:514363/1‑120 (MQ=255)                                                                                                          aGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              <  2:514363/120‑1 (MQ=255)                                                                                                                 tCGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcg                    <  2:901419/139‑1 (MQ=255)                                                                                                                  cGCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcgg                   <  2:792262/139‑1 (MQ=255)                                                                                                                   gCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                                             >  1:85916/1‑80 (MQ=255)                                                                                                                   gCTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                                             <  2:85916/80‑1 (MQ=255)                                                                                                                    cTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcggcg                 <  1:228093/139‑1 (MQ=255)                                                                                                                    cTCAGTGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcggcg                 <  2:41469/139‑1 (MQ=255)                                                                                                                         tGCGGGCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTatat            >  2:658710/1‑139 (MQ=255)                                                                                                                              gCACGTAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCa       >  2:800460/1‑139 (MQ=255)                                                                                                                                   tAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              >  1:187434/1‑95 (MQ=255)                                                                                                                                   tAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                              <  2:187434/95‑1 (MQ=255)                                                                                                                                   tAACACCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGtttt  >  2:1546034/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                           GCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCG‑‑‑‑ACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTT  >  NC_000913/1761384‑1761652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑