Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	183,215	C→T	G251G (GGC→GGT)	cdaR →	carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	183,215	0	C	T	100.0%	212.8 / NA	66	G251G (GGC→GGT)	cdaR	carbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/38);  total (28/38)

AACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCTGTGTTACT  >  NC_000913/183080‑183346                                                                                                                                        |                                                                                                                                    aaCCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAAc                                                                                                                                  <  2:466737/139‑1 (MQ=255)   ccGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTa                                                                                                                                >  1:503492/1‑139 (MQ=255)        aTGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTa                                                                                                                           <  2:371919/139‑1 (MQ=255)              gtgtTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTc                                                                                                                     >  1:297738/1‑139 (MQ=255)                 ttGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTg                                                                                                                  <  1:378374/139‑1 (MQ=255)                  tGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTgg                                                                                                                 <  2:244533/139‑1 (MQ=255)                       ccGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTa                                                                                                            <  1:843100/139‑1 (MQ=255)                            gTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       <  1:1265425/139‑1 (MQ=255)                            gTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       <  2:571250/139‑1 (MQ=255)                            gTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       <  2:1334464/139‑1 (MQ=255)                                 aCTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGTCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATc                                                                                                  <  1:1172927/139‑1 (MQ=255)                                     ttttGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGt                                                                                                             >  2:72690/1‑124 (MQ=255)                                     ttttGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGt                                                                                                             <  1:72690/124‑1 (MQ=255)                                               tGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       <  1:1471319/120‑1 (MQ=255)                                               tGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       >  2:1471319/1‑120 (MQ=255)                                                 ggATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGaaa                                                                                  >  1:129/1‑139 (MQ=255)                                                            atcatcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       <  1:805090/107‑1 (MQ=255)                                                            atcatcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       >  2:805090/1‑107 (MQ=255)                                                             tcatcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTa                                                                                                            <  1:732996/101‑1 (MQ=255)                                                             tcatcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTa                                                                                                            >  2:732996/1‑101 (MQ=255)                                                                tcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtggtg                                                                   <  2:297738/139‑1 (MQ=255)                                                                tcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtggtg                                                                   <  2:129/139‑1 (MQ=255)                                                                 cGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtggtgg                                                                  >  2:1243936/1‑139 (MQ=255)                                                                         aGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACT‑‑GTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCg                                                        >  2:423449/1‑139 (MQ=255)                                                                           ttGAACGACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCg                                                        >  1:747084/1‑139 (MQ=255)                                                                           ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                                      >  1:1232631/1‑77 (MQ=255)                                                                           ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                                      <  2:1232631/77‑1 (MQ=255)                                                                           ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       >  2:250643/1‑92 (MQ=255)                                                                           ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       <  1:250643/92‑1 (MQ=255)                                                                           ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTaaa                                                             >  2:1730630/1‑134 (MQ=255)                                                                           ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTaaa                                                             <  1:1730630/134‑1 (MQ=255)                                                                           ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCg                                                        <  2:1418484/139‑1 (MQ=255)                                                                           ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCg                                                        >  2:1036030/1‑139 (MQ=255)                                                                           ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGATAAACAGCg                                                        >  2:303864/1‑139 (MQ=255)                                                                                     gATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAAcgacga                                                                           >  1:1169057/1‑110 (MQ=255)                                                                                     gATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAAcgacga                                                                           <  2:1169057/110‑1 (MQ=255)                                                                                     gATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACT‑‑GTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAg                                            <  1:423449/139‑1 (MQ=255)                                                                                        tACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       <  2:1413378/79‑1 (MQ=255)                                                                                        tACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       >  1:1413378/1‑79 (MQ=255)                                                                                             gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       >  2:223770/1‑74 (MQ=255)                                                                                             gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       <  1:223770/74‑1 (MQ=255)                                                                                                tGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       <  2:610971/71‑1 (MQ=255)                                                                                                tGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                       >  1:610971/1‑71 (MQ=255)                                                                                                        tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtggtg                                                                   <  2:702153/99‑1 (MQ=255)                                                                                                        tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtggtg                                                                   >  1:702153/1‑99 (MQ=255)                                                                                                        tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGa                                                      <  2:1349047/112‑1 (MQ=255)                                                                                                        tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGa                                                      >  1:1349047/1‑112 (MQ=255)                                                                                                        tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                           <  1:1263435/139‑1 (MQ=255)                                                                                                        tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                           >  2:556045/1‑139 (MQ=255)                                                                                                        tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                           <  2:503492/139‑1 (MQ=255)                                                                                                        tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                           <  2:770211/139‑1 (MQ=255)                                                                                                        tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                           >  1:1091391/1‑139 (MQ=255)                                                                                                        tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                           <  2:1091391/139‑1 (MQ=255)                                                                                                         aCGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTa                                                                                                                           >  1:1465368/1‑42 (MQ=255)                                                                                                         aCGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTa                                                                                                                           <  2:1465368/42‑1 (MQ=255)                                                                                                         aCGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtgg                                                                     >  1:652491/1‑96 (MQ=255)                                                                                                         aCGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtgg                                                                     <  2:652491/96‑1 (MQ=255)                                                                                                              cAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATc                     >  2:240134/1‑139 (MQ=255)                                                                                                                gCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTg                   >  2:848540/1‑139 (MQ=255)                                                                                                                 cTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGa                                                      >  1:179600/1‑103 (MQ=255)                                                                                                                 cTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGa                                                      <  2:179600/103‑1 (MQ=255)                                                                                                                  tGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGAt                 <  1:1243936/139‑1 (MQ=255)                                                                                                                  tGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGAt                 <  1:556045/139‑1 (MQ=255)                                                                                                                       tttCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGATAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTAc            <  1:303864/139‑1 (MQ=255)                                                                                                                          cgcgTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCtg         <  2:747084/139‑1 (MQ=255)                                                                                                                                 cACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCTGTGTTACt  <  2:994017/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    AACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCTGTGTTACT  >  NC_000913/183080‑183346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑