Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,901,506	T→C	N27S (AAC→AGC)	yoaE ←	putative membrane protein/conserved protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,901,506	0	T	C	100.0%	225.9 / NA	62	N27S (AAC→AGC)	yoaE	putative membrane protein/conserved protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/36);  total (26/36)

GTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGTTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATG  >  NC_000913/1901371‑1901631                                                                                                                                        |                                                                                                                              gTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTAt                                                                                                                            >  1:839836/1‑139 (MQ=255)   cAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCg                                                                                                                          <  2:1746679/139‑1 (MQ=255)      cGTGACCATCCATGAAATCGGCGACAGCAG‑CCCATACGCATAATCAGCGCCAGTGATAACCCCAGCAAACACGCTTTATCGCGTTGTATTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATAc                                                                                                                      >  1:944420/1‑139 (MQ=255)      cGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATa                                                                                                                       >  1:1550156/1‑139 (MQ=255)        tGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATAcc                                                                                                                     <  2:1587805/139‑1 (MQ=255)           ccatccatGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAg                                                                                                                  >  2:1793053/1‑139 (MQ=255)             atccatGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGca                                                                                                                <  2:1561849/139‑1 (MQ=255)             atccatGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGca                                                                                                                <  2:1231480/139‑1 (MQ=255)              tccatGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGcac                                                                                                               >  2:1044269/1‑139 (MQ=255)               ccatGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGcaca                                                                                                              <  1:1044269/139‑1 (MQ=255)                        cAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATa                                                                                                                       >  2:245772/1‑121 (MQ=255)                        cAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATa                                                                                                                       <  1:245772/121‑1 (MQ=255)                         aGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGaa                                                                                                    >  1:390757/1‑139 (MQ=255)                          gCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGaac                                                                                                   >  2:887762/1‑139 (MQ=255)                           cGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGaaca                                                                                                  >  1:1325129/1‑139 (MQ=255)                           cGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGaaca                                                                                                  >  1:1724459/1‑139 (MQ=255)                              cagcagCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAacaaca                                                                                               <  1:469249/139‑1 (MQ=255)                                 cagcCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGc                                                                                            <  1:31846/139‑1 (MQ=255)                                         gACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATAcc                                                                                                                     <  1:1119785/106‑1 (MQ=255)                                         gACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATAcc                                                                                                                     >  2:1119785/1‑106 (MQ=255)                                         gACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGaacaa                                                                                                 >  2:696514/1‑126 (MQ=255)                                         gACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGaacaa                                                                                                 <  1:696514/126‑1 (MQ=255)                                         gACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAg                                                                                    >  1:340458/1‑139 (MQ=255)                                         gACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAg                                                                                    >  2:1382437/1‑139 (MQ=255)                                          aCGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGc                                                                                   <  1:1793053/139‑1 (MQ=255)                                           cGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGcc                                                                                  <  2:944420/139‑1 (MQ=255)                                            gCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGccc                                                                                 <  2:1325129/139‑1 (MQ=255)                                                         cAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAg                                                                    <  2:390757/139‑1 (MQ=255)                                                          aGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAgg                                                                   <  2:1518418/139‑1 (MQ=255)                                                          aGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAgg                                                                   <  2:571616/139‑1 (MQ=255)                                                           gTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAggg                                                                  <  1:344580/139‑1 (MQ=255)                                                           gTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAggg                                                                  <  2:979814/139‑1 (MQ=255)                                                            tGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAgggg                                                                 >  1:95301/1‑139 (MQ=255)                                                             gATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGt                                                                <  2:1724459/139‑1 (MQ=255)                                                                  ccccAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCAtt                                                           <  1:1382437/139‑1 (MQ=255)                                                                      aGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGaacaa                                                                                                 >  2:1723314/1‑97 (MQ=255)                                                                      aGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGaacaa                                                                                                 <  1:1723314/97‑1 (MQ=255)                                                                      aGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCTCCCAAATTGAGGGGTCCATTAAGa                                                       >  2:1000833/1‑139 (MQ=255)                                                                      aGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGccc                                                                            <  2:1520485/118‑1 (MQ=255)                                                                      aGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGccc                                                                            >  1:1520485/1‑118 (MQ=255)                                                                             gcgcTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAg                                                                    <  1:140616/119‑1 (MQ=255)                                                                             gcgcTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAg                                                                    >  2:140616/1‑119 (MQ=255)                                                                             gcgcTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCAt                                                >  2:218999/1‑139 (MQ=255)                                                                                cTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGAc                                             <  1:1490330/139‑1 (MQ=255)                                                                                cTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGAc                                             <  1:1000833/139‑1 (MQ=255)                                                                                     tCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCt                                        <  2:340458/139‑1 (MQ=255)                                                                                     tCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCt                                        <  1:887762/139‑1 (MQ=255)                                                                                      cgcgTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTc                                       <  1:1527785/139‑1 (MQ=255)                                                                                      cgcgTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTc                                       <  1:218999/139‑1 (MQ=255)                                                                                             ttttGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTa                                >  2:1764571/1‑139 (MQ=255)                                                                                             ttttGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTa                                <  2:536729/139‑1 (MQ=255)                                                                                                tGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAgg                             >  2:941162/1‑139 (MQ=255)                                                                                                       aGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAgg                                                                   <  2:512168/94‑1 (MQ=255)                                                                                                       aGTTTGTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAgg                                                                   >  1:512168/1‑94 (MQ=255)                                                                                                            gTCAGCAAGAATGGCGATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCg                 <  1:1764571/139‑1 (MQ=255)                                                                                                                           gATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa                                              <  1:709108/95‑1 (MQ=255)                                                                                                                           gATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGa                                              >  2:709108/1‑95 (MQ=255)                                                                                                                           gATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTa                                >  1:631587/1‑109 (MQ=255)                                                                                                                           gATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTa                                <  2:631587/109‑1 (MQ=255)                                                                                                                           gATGAAGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATg  <  2:839836/139‑1 (MQ=255)                                                                                                                               aaGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGc                                                                                   <  2:1635409/54‑1 (MQ=255)                                                                                                                               aaGACCAGGCTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGc                                                                                   >  1:1635409/1‑54 (MQ=255)                                                                                                                                        |                                                                                                                              GTCAGCGTGACCATCCATGAAATCAGCGACAGCAGCCCCAGACGCATAATCAGCGCCAGTGATAACCCCAGCAAACGCGCTTTATCGCGTTGTTTTGGCGGCAGTTTGTCAGCAAGAATGGCGATGAAGACCAGGTTATCGATACCCAGCACAATTTCGAGAACAACAAGCGTGAGTAGCCCCGCCCAAATTGAGGGGTCCATTAAGAATTCCATGACAAGCTCCTGCTTAAGGAATAGCTATTCGACGCCAGAAATAATG  >  NC_000913/1901371‑1901631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑