Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,934,813 A→G intergenic (‑209/+26) edd ← / ← zwf 6‑phosphogluconate dehydratase/glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,8130AG100.0% 158.0 / NA 44intergenic (‑209/+26)edd/zwf6‑phosphogluconate dehydratase/glucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

GTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATC  >  NC_000913/1934684‑1934944
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gTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATaa                                                                                                                            >  2:1701307/1‑139 (MQ=255)
    ccGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGa                                                                                                                        <  1:1353138/139‑1 (MQ=255)
     cGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGAt                                                                                                                       <  2:622117/139‑1 (MQ=255)
          cTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAgcg                                                                                                                  <  1:534422/139‑1 (MQ=255)
                    aaaaaCGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAgcgc                                                                                                                 <  2:401504/130‑1 (MQ=255)
                    aaaaaCGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAgcgc                                                                                                                 >  1:401504/1‑130 (MQ=255)
                            ttCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTc                                                                                                >  2:711012/1‑139 (MQ=255)
                                    gtgtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAgg                                                                                        <  1:711012/139‑1 (MQ=255)
                                      gtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGaa                                                                                      >  2:609919/1‑139 (MQ=255)
                                             aaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCAt                                                                               <  1:1701307/139‑1 (MQ=255)
                                               aaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTaa                                                                                                                                  <  2:1309221/86‑1 (MQ=255)
                                               aaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTaa                                                                                                                                  >  1:1309221/1‑86 (MQ=255)
                                               aaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAgcgc                                                                                                                 <  2:1246600/103‑1 (MQ=255)
                                               aaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAgcgc                                                                                                                 >  1:1246600/1‑103 (MQ=255)
                                                   ccTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACggg                                                                         <  1:1493446/139‑1 (MQ=255)
                                                            tGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCg                                                                <  1:1021613/139‑1 (MQ=255)
                                                                     aCGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAAc                                                                                                                                 >  1:300306/1‑65 (MQ=255)
                                                                     aCGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAAc                                                                                                                                 <  2:300306/65‑1 (MQ=255)
                                                                         ggCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt                                                                                                 >  2:924606/1‑93 (MQ=255)
                                                                         ggCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt                                                                                                 <  1:924606/93‑1 (MQ=255)
                                                                          gCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACg                                                  >  1:514385/1‑139 (MQ=255)
                                                                                 aGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcccc                                           <  1:609919/139‑1 (MQ=255)
                                                                                   tgtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAg                                         >  2:568489/1‑139 (MQ=255)
                                                                                    gtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCa                                                                                                                               >  1:295613/1‑52 (MQ=255)
                                                                                    gtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCa                                                                                                                               <  2:295613/52‑1 (MQ=255)
                                                                                    gtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAgg                                        >  1:942772/1‑139 (MQ=255)
                                                                                     tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcccc                                           <  1:1367667/135‑1 (MQ=255)
                                                                                     tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcccc                                           >  2:1367667/1‑135 (MQ=255)
                                                                                       aaaaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc                                     <  2:942772/139‑1 (MQ=255)
                                                                                           ttGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGc                                 <  2:514385/139‑1 (MQ=255)
                                                                                                         gcgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCg                   >  1:1449938/1‑139 (MQ=255)
                                                                                                          cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt                                                                                                 >  2:796898/1‑60 (MQ=255)
                                                                                                          cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt                                                                                                 <  1:796898/60‑1 (MQ=255)
                                                                                                          cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACggg                                                >  2:1621182/1‑109 (MQ=255)
                                                                                                          cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACggg                                                <  1:1621182/109‑1 (MQ=255)
                                                                                                          cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc                                     >  1:1777685/1‑120 (MQ=255)
                                                                                                          cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc                                     <  2:1777685/120‑1 (MQ=255)
                                                                                                               gATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGatat                                                                                                           <  1:1552867/45‑1 (MQ=255)
                                                                                                               gATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGatat                                                                                                           >  2:1552867/1‑45 (MQ=255)
                                                                                                                  cATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACg                                                                                    >  2:63280/1‑65 (MQ=255)
                                                                                                                  cATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACg                                                                                    <  1:63280/65‑1 (MQ=255)
                                                                                                                          cGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt                                                                                                 >  1:624556/1‑44 (MQ=255)
                                                                                                                          cGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt                                                                                                 <  2:624556/44‑1 (MQ=255)
                                                                                                                          cGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATc  >  2:1305940/1‑139 (MQ=255)
                                                                                                                                 |                                                                                                                                   
GTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATC  >  NC_000913/1934684‑1934944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑