Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,993,949	T→C	D253G (GAC→GGC)	uvrC ←	excinuclease UvrABC, endonuclease subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,993,949	0	T	C	100.0%	204.1 / NA	57	D253G (GAC→GGC)	uvrC	excinuclease UvrABC, endonuclease subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

CTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGT  >  NC_000913/1993818‑1994080                                                                                                                                    |                                                                                                                                    cTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGgtc                                                                                                                              <  2:762398/139‑1 (MQ=255)          cGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGt                                                                                                                     >  1:1132241/1‑139 (MQ=255)               aGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTgg                                                                                                                >  1:1773072/1‑139 (MQ=255)                 ttCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCg                                                                                                                       <  1:1122790/130‑1 (MQ=255)                 ttCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCg                                                                                                                       >  2:1122790/1‑130 (MQ=255)                    cGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACg                                                                                                           >  2:514991/1‑139 (MQ=255)                      tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGtcgtcg                                                                                                                          >  1:423264/1‑122 (MQ=255)                      tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGtcgtcg                                                                                                                          <  2:423264/122‑1 (MQ=255)                       aCCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAAt                                                                                                        <  1:1067184/139‑1 (MQ=255)                              ggCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGttttt                                                                                                 <  1:1374479/139‑1 (MQ=255)                              ggCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGttttt                                                                                                 >  2:1773557/1‑139 (MQ=255)                                                 tGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGgtcgt                                                                                                                            <  2:1231650/93‑1 (MQ=255)                                                 tGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGgtcgt                                                                                                                            >  1:1231650/1‑93 (MQ=255)                                                   cGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                  <  2:442683/133‑1 (MQ=255)                                                   cGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                  >  1:442683/1‑133 (MQ=255)                                                       tGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAAt                                                                        >  1:698724/1‑139 (MQ=255)                                                           gAGCACTTTGCCCTGACGAATGAACAAGACGTGTACACAGGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTgg                                                                    <  1:514991/139‑1 (MQ=255)                                                              cACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTcg                                                                 <  1:1339658/139‑1 (MQ=255)                                                              cACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTcg                                                                 <  1:1773557/139‑1 (MQ=255)                                                                   tGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGcc                                                                                                                        <  2:108572/79‑1 (MQ=255)                                                                   tGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGcc                                                                                                                        >  1:108572/1‑79 (MQ=255)                                                                   tGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGa                                                                                                               >  1:1343537/1‑88 (MQ=255)                                                                   tGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGa                                                                                                               <  2:1343537/88‑1 (MQ=255)                                                                                           ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTAt                                                                                                                   >  1:1613645/1‑60 (MQ=255)                                                                                           ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTAt                                                                                                                   <  2:1613645/60‑1 (MQ=255)                                                                                           ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAg                                    >  2:1684781/1‑139 (MQ=255)                                                                                           ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCCTCAAACTCCAg                                    >  2:1460620/1‑139 (MQ=255)                                                                                           ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGCCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAg                                    >  1:529444/1‑139 (MQ=255)                                                                                             acacAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGAt                                  <  2:1781055/139‑1 (MQ=255)                                                                                             acacAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGAt                                  >  2:1551373/1‑139 (MQ=255)                                                                                              cacaAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCCTCAAACTCCAGAtt                                 <  1:1460620/139‑1 (MQ=255)                                                                                                caAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTct                               >  2:1471828/1‑139 (MQ=255)                                                                                                  aGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCa                                            <  1:1537299/124‑1 (MQ=255)                                                                                                  aGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCa                                            >  2:1537299/1‑124 (MQ=255)                                                                                                   gCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAAtt                                                                       >  2:534209/1‑96 (MQ=255)                                                                                                   gCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAAtt                                                                       <  1:534209/96‑1 (MQ=255)                                                                                                   gCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctggc                            >  2:832397/1‑139 (MQ=255)                                                                                                       tGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGc                                                   >  1:310820/1‑112 (MQ=255)                                                                                                       tGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGc                                                   <  2:310820/112‑1 (MQ=255)                                                                                                          ccGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGt                     <  2:1531545/139‑1 (MQ=255)                                                                                                          ccGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGt                     <  1:324591/139‑1 (MQ=255)                                                                                                             cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                  >  2:662940/1‑75 (MQ=255)                                                                                                             cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                  <  1:662940/75‑1 (MQ=255)                                                                                                             cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctggctgg                         <  2:815732/132‑1 (MQ=255)                                                                                                             cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctggctgg                         >  1:815732/1‑132 (MQ=255)                                                                                                              aTCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                 >  2:1711611/1‑139 (MQ=255)                                                                                                              aTCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                 >  1:1747357/1‑139 (MQ=255)                                                                                                              aTCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                 >  1:1621425/1‑139 (MQ=255)                                                                                                               tCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCa                <  2:698724/139‑1 (MQ=255)                                                                                                                 gAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATa              <  1:1711611/139‑1 (MQ=255)                                                                                                                   aCGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGtt                    <  1:346316/131‑1 (MQ=255)                                                                                                                   aCGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGtt                    >  2:346316/1‑131 (MQ=255)                                                                                                                          acCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                         <  1:673687/39‑1 (MQ=255)                                                                                                                          acCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                         >  2:673687/1‑39 (MQ=38)                                                                                                                             aaTAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGt  <  2:177234/139‑1 (MQ=255)                                                                                                                              aTAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATa                                                           <  1:1311521/81‑1 (MQ=255)                                                                                                                              aTAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATa                                                           >  2:1311521/1‑81 (MQ=255)                                                                                                                                    |                                                                                                                                    CTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGT  >  NC_000913/1993818‑1994080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑