Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	184,257	C→T	*271* (TAG→TAA)	yaeI ←	phosphodiesterase with model substrate bis‑pNPP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	184,257	0	C	T	100.0%	179.8 / NA	57	*271* (TAG→TAA)	yaeI	phosphodiesterase with model substrate bis‑pNPP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

TTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACA  >  NC_000913/184140‑184392                                                                                                                      |                                                                                                                                        tttGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGtt                                                                                                                    <  1:1580373/139‑1 (MQ=255)   tggcctggccCGCCCGGCTCTACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTAc                                                                                                                  >  1:1056809/1‑139 (MQ=255)    ggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACt                                                                                                                 >  2:434534/1‑139 (MQ=255)          gcccgcccGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCggg                                                                                                           >  1:1506794/1‑139 (MQ=255)          gcccgcccGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCggg                                                                                                           >  1:1644729/1‑139 (MQ=255)               cccgGCTCGACTGTGTTCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACTTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCa                                                                                                      >  1:1201277/1‑139 (MQ=255)               cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCa                                                                                                      >  1:812362/1‑139 (MQ=255)               cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCa                                                                                                      >  2:1103065/1‑139 (MQ=255)                    cTCGCCTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                        <  2:1162111/132‑1 (MQ=255)                    cTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                        >  1:1162111/1‑132 (MQ=255)                    cTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCa                                                                                                 >  1:582143/1‑139 (MQ=255)                    cTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCa                                                                                                 >  1:355897/1‑139 (MQ=255)                           gtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  2:40007/139‑1 (MQ=255)                              aTCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAAcc                                                                                       >  2:1451670/1‑139 (MQ=255)                                       aGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACt                                                                              <  1:434534/139‑1 (MQ=255)                                          cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc                                                                           <  2:1775062/139‑1 (MQ=255)                                                 ttcCAGTCTTCTATCATGCCGTAAAAATCAAAAAATTAACTGCTTTATTCTGGTGATAAAATTCACGCTTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCACACAAACTGCccacgcca                                                                    <  1:267329/139‑1 (MQ=255)                                                     aGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:1451670/139‑1 (MQ=255)                                                     aGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:1103065/139‑1 (MQ=255)                                                     aGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGACTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACTCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:1201277/139‑1 (MQ=255)                                                      gACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGt                                                               >  1:622658/1‑139 (MQ=255)                                                       aCTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   <  2:957836/134‑1 (MQ=255)                                                       aCTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   >  1:957836/1‑134 (MQ=255)                                                           cTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACAcc                                                                                                                                   >  1:1509283/1‑66 (MQ=255)                                                           cTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACAcc                                                                                                                                   <  2:1509283/66‑1 (MQ=255)                                                                 tGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTc                                                                                                  >  2:950447/1‑93 (MQ=255)                                                                 tGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTc                                                                                                  <  1:950447/93‑1 (MQ=255)                                                                 tGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtgt                                                    >  1:153835/1‑139 (MQ=255)                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAg                                                        >  2:566674/1‑133 (MQ=255)                                                                   gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAg                                                        <  1:566674/133‑1 (MQ=255)                                                                     gTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCt                                                <  2:1506794/139‑1 (MQ=255)                                                                      tAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc                                                                                                                          <  1:1694864/64‑1 (MQ=255)                                                                      tAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGc                                                                                                                          >  2:1694864/1‑64 (MQ=255)                                                                      tAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   <  2:485817/119‑1 (MQ=255)                                                                      tAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   >  1:485817/1‑119 (MQ=255)                                                                             cAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaa                                        <  2:812362/139‑1 (MQ=255)                                                                              aaaaaaTTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaaa                                       <  2:582143/139‑1 (MQ=255)                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTGCTTCCGGGCGGCAGTTCAGACGCAAACCa                                                                                      >  1:1183953/1‑69 (MQ=255)                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCa                                                                                      <  2:1183953/69‑1 (MQ=255)                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc                                                                           >  2:886069/1‑80 (MQ=255)                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc                                                                           <  1:886069/80‑1 (MQ=255)                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   >  2:1336701/1‑88 (MQ=255)                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   <  1:1336701/88‑1 (MQ=255)                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAAc                >  2:961036/1‑139 (MQ=255)                                                                                                     gTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAAc                >  2:1412370/1‑139 (MQ=255)                                                                                                          aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCGGTTCAGACGCa                                                                                           <  1:1042972/59‑1 (MQ=255)                                                                                                          aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCa                                                                                           >  2:1042972/1‑59 (MQ=255)                                                                                                          aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   >  2:349120/1‑83 (MQ=255)                                                                                                          aaaaTTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   <  1:349120/83‑1 (MQ=255)                                                                                                               tCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACg                                                                                             <  1:1484288/52‑1 (MQ=255)                                                                                                               tCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACg                                                                                             >  2:1484288/1‑52 (MQ=255)                                                                                                               tCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaaa                                       >  2:1510577/1‑106 (MQ=255)                                                                                                               tCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaaa                                       >  2:633887/1‑106 (MQ=255)                                                                                                               tCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaaa                                       <  1:1510577/106‑1 (MQ=255)                                                                                                               tCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaaa                                       <  1:633887/106‑1 (MQ=255)                                                                                                                   gATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACa  <  2:622658/139‑1 (MQ=255)                                                                                                                   gATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACa  <  2:1644729/139‑1 (MQ=255)                                                                                                                      |                                                                                                                                        TTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACA  >  NC_000913/184140‑184392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑