Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,160,196 | T→C | V604A (GTG→GCG) | mdtC → | multidrug efflux system, subunit C |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,160,196 | 0 | T | C | 98.2% | 195.5 / ‑3.8 | 56 | V604A (GTG→GCG) | mdtC | multidrug efflux system, subunit C |
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (28/27); minor base G (1/0); total (29/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 |
TTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATGGCGGTA > NC_000913/2160063‑2160326 | ttGATGGGCGGGATTAAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACa > 1:1202378/1‑139 (MQ=255) gggCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCggg < 1:1766877/139‑1 (MQ=255) cGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg < 2:480841/139‑1 (MQ=255) cGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg < 1:853545/139‑1 (MQ=255) aGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt < 1:196759/139‑1 (MQ=255) aTCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCt > 2:1249357/1‑139 (MQ=255) ttCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATACTGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg > 1:497052/1‑115 (MQ=255) ttCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg < 2:497052/115‑1 (MQ=255) cGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGcga > 2:8699/1‑139 (MQ=255) ttCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGcgacga > 1:881344/1‑139 (MQ=255) cAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGaa < 1:677841/98‑1 (MQ=255) cAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGaa > 2:677841/1‑98 (MQ=255) cAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg < 2:304312/108‑1 (MQ=255) cAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg > 1:304312/1‑108 (MQ=255) tGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt < 2:1531894/109‑1 (MQ=255) tGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt > 1:1531894/1‑109 (MQ=255) gcgGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAAc < 2:881344/139‑1 (MQ=255) tGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGATGATGTTTATCACCCTAAAGCCACGCGACGAACGCAGCGAAACGGCgcagca > 2:1082972/1‑139 (MQ=255) gATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGGGGTTCGCGAGCGAACAGCGGGATGATGTTTAt > 1:1186014/1‑92 (MQ=255) gATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCggg < 2:906584/81‑1 (MQ=255) gATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCggg > 1:906584/1‑81 (MQ=255) gATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt < 2:1186014/92‑1 (MQ=255) tttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCAc > 2:1503422/1‑104 (MQ=255) tttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCAc < 1:1503422/104‑1 (MQ=255) tttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATc > 2:620139/1‑139 (MQ=255) tCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGa > 2:175332/1‑139 (MQ=255) tCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCA‑CCTCAAGCCACGCGACGAACGCAGCGAAACGgcg < 1:1625419/124‑1 (MQ=255) tCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCA‑CCTCAAGCCACGCGACGAACGCAGCGAAACGgcg > 2:1625419/1‑124 (MQ=255) tGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCg > 2:66789/1‑139 (MQ=255) tCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGa < 2:607895/127‑1 (MQ=255) tCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGa > 1:607895/1‑127 (MQ=255) ccGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTa < 1:620139/139‑1 (MQ=255) gACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt < 1:1324691/71‑1 (MQ=255) gACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt > 2:1324691/1‑71 (MQ=255) tCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACgg > 2:676350/1‑101 (MQ=255) tCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACgg < 1:676350/101‑1 (MQ=255) tGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAAcc > 1:581088/1‑139 (MQ=255) gATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGga > 2:931945/1‑46 (MQ=255) gATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGga < 1:931945/46‑1 (MQ=255) gATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAAc < 2:1261076/136‑1 (MQ=255) gATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAAc > 1:1261076/1‑136 (MQ=255) gATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCgg > 2:973937/1‑139 (MQ=255) tGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGaaa > 2:62797/1‑83 (MQ=255) tGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGaaa < 1:62797/83‑1 (MQ=255) tGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCg > 1:880118/1‑139 (MQ=255) gTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGa < 1:93370/139‑1 (MQ=255) gTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGa < 2:880118/139‑1 (MQ=255) aCCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGaaa > 2:601071/1‑79 (MQ=255) aCCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGaaa < 1:601071/79‑1 (MQ=255) ccGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACgg < 2:1173996/81‑1 (MQ=255) ccGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCt > 2:82473/1‑139 (MQ=255) ccGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCCACGAACGCAGCGAAACgg > 1:1173996/1‑81 (MQ=255) ggCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATgg < 1:1249357/139‑1 (MQ=255) ggCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATgg < 2:1617867/139‑1 (MQ=255) ttCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATGGCGGTa > 1:1411195/1‑139 (MQ=255) ttCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATGGCGGTa < 1:175332/139‑1 (MQ=255) | TTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATGGCGGTA > NC_000913/2160063‑2160326 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |