Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,160,196 T→C V604A (GTG→GCG)  mdtC → multidrug efflux system, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,160,1960TC98.2% 195.5 / ‑3.8 56V604A (GTG→GCG) mdtCmultidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (28/27);  minor base G (1/0);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

TTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATGGCGGTA  >  NC_000913/2160063‑2160326
                                                                                                                                     |                                                                                                                                  
ttGATGGGCGGGATTAAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACa                                                                                                                               >  1:1202378/1‑139 (MQ=255)
     gggCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCggg                                                                                                                          <  1:1766877/139‑1 (MQ=255)
        cGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg                                                                                                                       <  2:480841/139‑1 (MQ=255)
        cGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg                                                                                                                       <  1:853545/139‑1 (MQ=255)
                aGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt                                                                                                               <  1:196759/139‑1 (MQ=255)
                      aTCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCt                                                                                                         >  2:1249357/1‑139 (MQ=255)
                                ttCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATACTGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg                                                                                                                       >  1:497052/1‑115 (MQ=255)
                                ttCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg                                                                                                                       <  2:497052/115‑1 (MQ=255)
                                  cGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGcga                                                                                             >  2:8699/1‑139 (MQ=255)
                                     ttCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGcgacga                                                                                          >  1:881344/1‑139 (MQ=255)
                                       cAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGaa                                                                                                                                 <  1:677841/98‑1 (MQ=255)
                                       cAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGaa                                                                                                                                 >  2:677841/1‑98 (MQ=255)
                                       cAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg                                                                                                                       <  2:304312/108‑1 (MQ=255)
                                       cAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGgatg                                                                                                                       >  1:304312/1‑108 (MQ=255)
                                              tGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt                                                                                                               <  2:1531894/109‑1 (MQ=255)
                                              tGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt                                                                                                               >  1:1531894/1‑109 (MQ=255)
                                                 gcgGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAAc                                                                              <  2:881344/139‑1 (MQ=255)
                                                          tGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGGGAACAGCGGGATGATGTTTATCACCCTAAAGCCACGCGACGAACGCAGCGAAACGGCgcagca                                                                     >  2:1082972/1‑139 (MQ=255)
                                                               gATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGGGGTTCGCGAGCGAACAGCGGGATGATGTTTAt                                                                                                               >  1:1186014/1‑92 (MQ=255)
                                                               gATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCggg                                                                                                                          <  2:906584/81‑1 (MQ=255)
                                                               gATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCggg                                                                                                                          >  1:906584/1‑81 (MQ=255)
                                                               gATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt                                                                                                               <  2:1186014/92‑1 (MQ=255)
                                                                 tttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCAc                                                                                                 >  2:1503422/1‑104 (MQ=255)
                                                                 tttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCAc                                                                                                 <  1:1503422/104‑1 (MQ=255)
                                                                 tttCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATc                                                              >  2:620139/1‑139 (MQ=255)
                                                                   tCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGa                                                            >  2:175332/1‑139 (MQ=255)
                                                                   tCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCTCAAGCCACGCGACGAACGCAGCGAAACGgcg                                                                          <  1:1625419/124‑1 (MQ=255)
                                                                   tCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCTCAAGCCACGCGACGAACGCAGCGAAACGgcg                                                                          >  2:1625419/1‑124 (MQ=255)
                                                                      tGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCg                                                         >  2:66789/1‑139 (MQ=255)
                                                                               tCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGa                                                            <  2:607895/127‑1 (MQ=255)
                                                                               tCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGa                                                            >  1:607895/1‑127 (MQ=255)
                                                                                ccGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTa                                               <  1:620139/139‑1 (MQ=255)
                                                                                    gACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt                                                                                                               <  1:1324691/71‑1 (MQ=255)
                                                                                    gACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTAt                                                                                                               >  2:1324691/1‑71 (MQ=255)
                                                                                         tCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACgg                                                                            >  2:676350/1‑101 (MQ=255)
                                                                                         tCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACgg                                                                            <  1:676350/101‑1 (MQ=255)
                                                                                                 tGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAAcc                              >  1:581088/1‑139 (MQ=255)
                                                                                                   gATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGga                                                                                                                         >  2:931945/1‑46 (MQ=255)
                                                                                                   gATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGga                                                                                                                         <  1:931945/46‑1 (MQ=255)
                                                                                                   gATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAAc                               <  2:1261076/136‑1 (MQ=255)
                                                                                                   gATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAAc                               >  1:1261076/1‑136 (MQ=255)
                                                                                                   gATAATGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCgg                            >  2:973937/1‑139 (MQ=255)
                                                                                                        tGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGaaa                                                                               >  2:62797/1‑83 (MQ=255)
                                                                                                        tGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGaaa                                                                               <  1:62797/83‑1 (MQ=255)
                                                                                                        tGTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCg                       >  1:880118/1‑139 (MQ=255)
                                                                                                         gTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGa                      <  1:93370/139‑1 (MQ=255)
                                                                                                         gTCACCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGa                      <  2:880118/139‑1 (MQ=255)
                                                                                                            aCCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGaaa                                                                               >  2:601071/1‑79 (MQ=255)
                                                                                                            aCCGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGaaa                                                                               <  1:601071/79‑1 (MQ=255)
                                                                                                             ccGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACgg                                                                            <  2:1173996/81‑1 (MQ=255)
                                                                                                             ccGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCt                  >  2:82473/1‑139 (MQ=255)
                                                                                                             ccGGCTTTACAGGCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCCACGAACGCAGCGAAACgg                                                                            >  1:1173996/1‑81 (MQ=255)
                                                                                                                        ggCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATgg       <  1:1249357/139‑1 (MQ=255)
                                                                                                                        ggCGGTTCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATgg       <  2:1617867/139‑1 (MQ=255)
                                                                                                                             ttCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATGGCGGTa  >  1:1411195/1‑139 (MQ=255)
                                                                                                                             ttCGCGAGCGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATGGCGGTa  <  1:175332/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                  
TTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGTCACCGGCTTTACAGGCGGTTCGCGAGTGAACAGCGGGATGATGTTTATCACCCTCAAGCCACGCGACGAACGCAGCGAAACGGCGCAGCAAATTATCGACCGTCTGCGCGTAAAACTGGCGAAAGAACCGGGGGCGAATCTGTTCCTGATGGCGGTA  >  NC_000913/2160063‑2160326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: