Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 28,269 C→T intergenic (+62/‑105) rihC → / → dapB ribonucleoside hydrolase 3/dihydrodipicolinate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091328,2690CT100.0% 195.6 / NA 61intergenic (+62/‑105)rihC/dapBribonucleoside hydrolase 3/dihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/28);  total (33/28)

AGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCCGCGTTGCCATC  >  NC_000913/28137‑28403
                                                                                                                                    |                                                                                                                                      
aggaggCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGttt                                                                                                                                  >  1:471193/1‑139 (MQ=255)
    ggCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCa                                                                                                                              <  1:1085067/139‑1 (MQ=255)
       aTTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTa                                                                                                                           >  1:212679/1‑139 (MQ=255)
       aTTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTa                                                                                                                           >  2:203307/1‑139 (MQ=255)
                ggATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAAt                                                                                                                  >  1:736142/1‑139 (MQ=255)
                ggATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAAt                                                                                                                  >  1:991750/1‑139 (MQ=255)
                ggATGTGAAAGGCTTCCAGCAGTGGGTGACTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCa                                                                                                                              <  2:1539788/127‑1 (MQ=255)
                ggATGTGAAAGGCTTCCAGCAGTGGGTGACTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCa                                                                                                                              >  1:1539788/1‑127 (MQ=255)
                     tgAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCa                                                                                                             >  1:3026/1‑139 (MQ=255)
                           gCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGttt                                                                                                       <  2:688214/139‑1 (MQ=255)
                               ccAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTa                                                                                                                           >  2:849799/1‑115 (MQ=255)
                               ccAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTa                                                                                                                           <  1:849799/115‑1 (MQ=255)
                               ccAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAt                                                                                                                         >  2:1273171/1‑117 (MQ=255)
                               ccAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAt                                                                                                                         <  1:1273171/117‑1 (MQ=255)
                                   cagTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctgg                                                                                               <  2:471193/139‑1 (MQ=255)
                                     gtgggtggCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTggcg                                                                                             <  2:3026/139‑1 (MQ=255)
                                             cTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAt                                                                                                                         <  1:967309/103‑1 (MQ=255)
                                             cTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAt                                                                                                                         >  2:967309/1‑103 (MQ=255)
                                             cTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCg                                                                                     <  1:203307/139‑1 (MQ=255)
                                                       ggCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGt                                                                           <  2:991750/139‑1 (MQ=255)
                                                            tGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAttt                                                                                                                       <  1:269821/90‑1 (MQ=255)
                                                            tGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAttt                                                                                                                       >  2:269821/1‑90 (MQ=255)
                                                                  cgtAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGgcggcg                                                                                          <  1:1683588/113‑1 (MQ=255)
                                                                  cgtAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGgcggcg                                                                                          >  2:1683588/1‑113 (MQ=255)
                                                                    tAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACg                                                              <  2:114157/139‑1 (MQ=255)
                                                                           tCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTg                                                                                                                                     <  1:450970/61‑1 (MQ=255)
                                                                           tCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTg                                                                                                                                     >  2:450970/1‑61 (MQ=255)
                                                                                tGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAAt                                                  >  2:369688/1‑139 (MQ=255)
                                                                                     ttGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCg                                                                                     >  2:147533/1‑99 (MQ=255)
                                                                                     ttGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCg                                                                                     <  1:147533/99‑1 (MQ=255)
                                                                                     ttGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACa                                             >  2:43174/1‑139 (MQ=255)
                                                                                      tGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACaa                                            <  2:212679/139‑1 (MQ=255)
                                                                                          aTGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAttt                                                                                                                       <  1:1432167/60‑1 (MQ=255)
                                                                                          aTGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAAttt                                                                                                                       >  2:1432167/1‑60 (MQ=255)
                                                                                            gCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctgg                                                                                               <  2:1211143/82‑1 (MQ=255)
                                                                                            gCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTtggctgg                                                                                               >  1:1211143/1‑82 (MQ=255)
                                                                                            gCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTActc                                                                      <  2:1640947/107‑1 (MQ=255)
                                                                                            gCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTActc                                                                      >  1:1640947/1‑107 (MQ=255)
                                                                                            gCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGaaa                                                                 >  1:890344/1‑112 (MQ=255)
                                                                                            gCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGaaa                                                                 <  2:890344/112‑1 (MQ=255)
                                                                                                  attcatCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCt                                >  2:526772/1‑139 (MQ=255)
                                                                                                  attcatCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCt                                <  1:526772/139‑1 (MQ=255)
                                                                                                     catCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCATAGCGATGCGCTGGTTACTCTGaaa                                                                 <  2:340525/103‑1 (MQ=255)
                                                                                                     catCGACTCATGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCATAGCGATGCGCTGGTTACTCTGaaa                                                                 >  1:340525/1‑103 (MQ=255)
                                                                                                              aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCa                                                                                                             >  1:608627/1‑50 (MQ=255)
                                                                                                              aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCa                                                                                                             <  2:608627/50‑1 (MQ=255)
                                                                                                              aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGt                                                            <  2:916897/99‑1 (MQ=255)
                                                                                                              aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGt                                                            >  1:916897/1‑99 (MQ=255)
                                                                                                              aTGCCTTTCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCa                    >  1:1016011/1‑139 (MQ=255)
                                                                                                                     tCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCa                           <  1:1098540/125‑1 (MQ=255)
                                                                                                                     tCACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATcc             >  2:1503840/1‑139 (MQ=255)
                                                                                                                     tCACTGAGATCCCTCTCTGTTTATTATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCa                           >  2:1098540/1‑125 (MQ=255)
                                                                                                                      cACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGAt                                                                                   <  2:10330/68‑1 (MQ=255)
                                                                                                                      cACTGATATCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGAt                                                                                   >  1:10330/1‑68 (MQ=255)
                                                                                                                           atatCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATgc                                                                                 <  2:1553926/65‑1 (MQ=255)
                                                                                                                           atatCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATgc                                                                                 >  1:1553926/1‑65 (MQ=255)
                                                                                                                           atatCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTActc                                                                      <  2:1424272/76‑1 (MQ=255)
                                                                                                                           atatCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTActc                                                                      >  1:1424272/1‑76 (MQ=255)
                                                                                                                           atatCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCCGCGTTg       >  1:1318677/1‑139 (MQ=255)
                                                                                                                           atatCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCCGCGTTg       >  1:1302158/1‑139 (MQ=255)
                                                                                                                             atCCCTCTCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCCGCGTTGcc     >  1:1697078/1‑139 (MQ=255)
                                                                                                                              tccctctcTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACaaaa                                          >  2:1307599/1‑101 (MQ=255)
                                                                                                                              tccctctcTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACaaaa                                          <  1:1307599/101‑1 (MQ=255)
                                                                                                                                cctctctGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCCGCGTTGCCATc  >  2:1150320/1‑139 (MQ=255)
                                                                                                                                 ctctctGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGt                                                                           >  1:96157/1‑65 (MQ=255)
                                                                                                                                 ctctctGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGt                                                                           <  2:96157/65‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
AGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAACCTGTCACATGTTATTGGCATGCAGTCATTCATCGACTCATGCCTTTCACTGATATCCCTCCCTGTTTATCATTAATTTCTAATTATCAGCGTTTTTGGCTGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACGGTCTATGCAAATTAACAAAAGAGAATAGCTATGCATGATGCAAACATCCGCGTTGCCATC  >  NC_000913/28137‑28403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑