Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,318,019	G→A	R620C (CGT→TGT)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,318,019	0	G	A	100.0%	149.7 / NA	47	R620C (CGT→TGT)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/22);  total (25/22)

ATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCA  >  NC_000913/2317883‑2318147                                                                                                                                         |                                                                                                                                 aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGa                                                                                                                                <  1:113600/139‑1 (MQ=255)         ttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAgagaga                                                                                                                        <  2:503570/139‑1 (MQ=255)                                cGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 <  1:127211/139‑1 (MQ=255)                                cGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 <  2:900931/139‑1 (MQ=255)                                   tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaa                                                                                              >  1:857438/1‑139 (MQ=255)                                                    tGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 <  1:872279/119‑1 (MQ=255)                                                    tGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 >  2:872279/1‑119 (MQ=255)                                                     gTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATc                                                                            >  1:367516/1‑139 (MQ=255)                                                      tACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCg                                                                           <  2:1536077/139‑1 (MQ=255)                                                           ccGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGaa                                                                      <  1:423827/139‑1 (MQ=255)                                                              ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                         <  2:1019869/101‑1 (MQ=255)                                                              ttCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCcgcg                                                                                                         >  1:1019869/1‑101 (MQ=255)                                                                ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTa                                                                 >  1:520381/1‑139 (MQ=255)                                                                          aaaaaggATCAAACAAGGCCACCCCTTCTTTCGCCGGTATCCCCACCCCGGTAACCCGAACACAAAAAAGAAAAAAATCGCCAACCGCGCCAACAAGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACt                                                       <  1:298621/134‑1 (MQ=255)                                                                                  tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGt                                               >  2:437178/1‑139 (MQ=255)                                                                                  tCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGt                                               >  2:298621/1‑139 (MQ=255)                                                                                     aaCAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTgg                                            >  2:412359/1‑139 (MQ=255)                                                                                         aGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                      <  2:1381573/77‑1 (MQ=255)                                                                                         aGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGa                                                                                                      >  1:1381573/1‑77 (MQ=255)                                                                                            gcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGAc                                     <  2:637811/139‑1 (MQ=255)                                                                                               ccacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaaa                                                                                            <  1:371893/81‑1 (MQ=255)                                                                                               ccacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaaa                                                                                            >  2:371893/1‑81 (MQ=255)                                                                                               ccacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata                                                                                       <  1:760264/86‑1 (MQ=255)                                                                                               ccacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata                                                                                       >  2:760264/1‑86 (MQ=255)                                                                                                 acTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACctgct                                >  2:835943/1‑139 (MQ=255)                                                                                                  cttcttTCGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACctgctg                               >  1:864699/1‑139 (MQ=255)                                                                                                         cGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata                                                                                       >  1:1530753/1‑76 (MQ=255)                                                                                                         cGCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata                                                                                       <  2:1530753/76‑1 (MQ=255)                                                                                                          gCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 <  2:944800/65‑1 (MQ=255)                                                                                                          gCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 >  1:944800/1‑65 (MQ=255)                                                                                                          gCCGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCa                       >  1:1781664/1‑139 (MQ=255)                                                                                                            cGGTATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATc                     >  2:575524/1‑139 (MQ=255)                                                                                                            cGGTATCCCCACGCCGATATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                    >  1:1391879/1‑108 (MQ=255)                                                                                                            cGGTATCCCCACGCCGATATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCaa                                                    <  2:1391879/108‑1 (MQ=255)                                                                                                               tATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGc                                                                                   <  2:819072/74‑1 (MQ=255)                                                                                                               tATCCCCACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGc                                                                                   >  1:819072/1‑74 (MQ=255)                                                                                                                   cccACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 <  1:1719734/56‑1 (MQ=255)                                                                                                                   cccACGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATg                                                                                                 >  2:1719734/1‑56 (MQ=255)                                                                                                                      aCGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGt                                               >  2:230608/1‑103 (MQ=255)                                                                                                                      aCGCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGt                                               <  1:230608/103‑1 (MQ=255)                                                                                                                        gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACt                                                       >  1:520894/1‑93 (MQ=255)                                                                                                                        gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACt                                                       <  2:520894/93‑1 (MQ=255)                                                                                                                        gCCGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCAttt         >  2:1556644/1‑139 (MQ=255)                                                                                                                         ccGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGt                                                           >  2:754805/1‑88 (MQ=255)                                                                                                                         ccGGTATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGt                                                           <  1:754805/88‑1 (MQ=255)                                                                                                                             tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata                                                                                       <  2:1542987/56‑1 (MQ=255)                                                                                                                             tATCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata                                                                                       >  1:1542987/1‑56 (MQ=255)                                                                                                                               tCGCGAACACAGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa  <  1:437178/139‑1 (MQ=255)                                                                                                                                      cacaGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTa                                                                 >  1:991439/1‑69 (MQ=255)                                                                                                                                      cacaGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCa  <  1:1690661/132‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 ATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAACGCATCGGGTCGCCATTTAAGGCCA  >  NC_000913/2317883‑2318147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑