Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,358,163	G→A	A228V (GCG→GTG)	rhmA ←	2‑keto‑3‑deoxy‑L‑rhamnonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,358,163	0	G	A	100.0%	219.6 / NA	68	A228V (GCG→GTG)	rhmA	2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/29);  total (39/29)

CTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGCCCAA  >  NC_000913/2358034‑2358296                                                                                                                                  |                                                                                                                                      cTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCAtt                                                                                                                              >  1:17500/1‑139 (MQ=255)      atatCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  2:889851/139‑1 (MQ=255)       tatCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTgcg                                                                                                                        <  2:17500/139‑1 (MQ=255)                 tACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAgg                                                                                                              >  1:108315/1‑139 (MQ=255)                   ccTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAg                                                                                                            >  2:695397/1‑139 (MQ=255)                     ttttATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGcc                                                                                                          >  1:524619/1‑139 (MQ=255)                       ttATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCcac                                                                                                        >  1:916946/1‑139 (MQ=255)                       ttATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCcac                                                                                                        >  2:123039/1‑139 (MQ=255)                              tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGa                                                                                                 >  2:549527/1‑139 (MQ=255)                              tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGa                                                                                                 >  1:559433/1‑139 (MQ=255)                              tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGa                                                                                                 >  2:711161/1‑139 (MQ=255)                                     tttttGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAg                                                                                          >  2:1010576/1‑139 (MQ=255)                                           ccTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAg                                                                                          <  1:1542709/133‑1 (MQ=255)                                           ccTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAg                                                                                          >  2:1542709/1‑133 (MQ=255)                                            cTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCttt                                                                                   <  2:916946/139‑1 (MQ=255)                                               aTTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTAcc                                                                                >  1:1021903/1‑139 (MQ=255)                                               aTTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTAcc                                                                                >  2:953980/1‑139 (MQ=255)                                                 ttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                              <  2:524619/139‑1 (MQ=255)                                                 ttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                              <  1:700988/139‑1 (MQ=255)                                                 ttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                              <  1:713914/139‑1 (MQ=255)                                                      cATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCgcagca                                                                         <  1:695397/139‑1 (MQ=255)                                                         cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAgg                                                                                                                                  >  2:1390264/1‑79 (MQ=255)                                                         cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAgg                                                                                                                                  <  1:1390264/79‑1 (MQ=255)                                                         cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  1:208232/88‑1 (MQ=255)                                                         cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         >  2:208232/1‑88 (MQ=255)                                                         cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACgg                                                                      >  1:544632/1‑139 (MQ=255)                                                          gCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGa                                                                     >  2:618444/1‑139 (MQ=255)                                                          gCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGa                                                                     >  2:1254918/1‑139 (MQ=255)                                                              gTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATccg                                                                 <  1:123039/139‑1 (MQ=255)                                                                         aGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTgg                                                      >  2:1246367/1‑139 (MQ=255)                                                                          gggCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGt                                                     >  1:748481/1‑139 (MQ=255)                                                                               tCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAGAGTTCGCTCCCCACAcc                                                                                                                                     <  1:1771555/54‑1 (MQ=255)                                                                               tCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGcc                                                                                                                      >  1:1674922/1‑69 (MQ=255)                                                                               tCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGcc                                                                                                                      <  2:1674922/69‑1 (MQ=255)                                                                               tCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCaa                                                <  1:711161/139‑1 (MQ=255)                                                                                cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  1:1430682/65‑1 (MQ=255)                                                                                cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         >  2:1430682/1‑65 (MQ=255)                                                                                cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCaat                                               >  1:660520/1‑139 (MQ=255)                                                                                        agCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCt                                       <  2:23628/139‑1 (MQ=255)                                                                                          cATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         >  2:863957/1‑55 (MQ=255)                                                                                          cATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  1:863957/55‑1 (MQ=255)                                                                                             cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         >  2:1402974/1‑52 (MQ=255)                                                                                             cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  1:1040050/52‑1 (MQ=255)                                                                                             cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         >  2:1040050/1‑52 (MQ=255)                                                                                             cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  1:1402974/52‑1 (MQ=255)                                                                                             cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTAcc                                                                                <  2:1243746/93‑1 (MQ=255)                                                                                             cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTAcc                                                                                >  1:1243746/1‑93 (MQ=255)                                                                                             cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACt                                  >  1:1281342/1‑139 (MQ=255)                                                                                               tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc                                >  2:18240/1‑139 (MQ=255)                                                                                               tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc                                >  1:988210/1‑139 (MQ=255)                                                                                               tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc                                >  1:1124361/1‑139 (MQ=255)                                                                                                  cAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATccg                                                                 >  2:807071/1‑103 (MQ=255)                                                                                                  cAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATccg                                                                 <  1:807071/103‑1 (MQ=255)                                                                                                    aCGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGt                           >  2:187324/1‑139 (MQ=255)                                                                                                      gCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGc                         <  2:988210/139‑1 (MQ=255)                                                                                                       ccAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGcc                        <  1:618444/139‑1 (MQ=255)                                                                                                       ccAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGcc                        <  1:1246367/139‑1 (MQ=255)                                                                                                          aCAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCgg                     >  2:1706972/1‑139 (MQ=255)                                                                                                           cAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGc                    <  2:1124361/139‑1 (MQ=255)                                                                                                           cAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGc                    <  1:1706972/139‑1 (MQ=255)                                                                                                                        cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACt                                                        <  1:951553/90‑1 (MQ=255)                                                                                                                        cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACt                                                        >  2:951553/1‑90 (MQ=255)                                                                                                                        cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGt                                                     >  1:762352/1‑93 (MQ=255)                                                                                                                        cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGt                                                     <  2:762352/93‑1 (MQ=255)                                                                                                                        cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAg       >  1:526784/1‑139 (MQ=255)                                                                                                                        cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAg       >  2:1500376/1‑139 (MQ=255)                                                                                                                         gCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGc      >  2:891434/1‑139 (MQ=255)                                                                                                                             cccACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCAGGTAGCCCaa  <  1:18240/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      CTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGCCCAA  >  NC_000913/2358034‑2358296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑