Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,472,605	G→A	R93W (CGG→TGG)	yfdN ←	CPS‑53 (KpLE1) prophage, uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,472,605	0	G	A	100.0%	205.1 / NA	65	R93W (CGG→TGG)	yfdN	CPS‑53 (KpLE1) prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/36);  total (29/36)

GACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCTGTG  >  NC_000913/2472470‑2472710                                                                                                                                        |                                                                                                          gacgCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACgg                                                                                                        >  2:592151/1‑139 (MQ=255)  acgCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACggg                                                                                                       >  1:902997/1‑139 (MQ=255)    gcgcCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTc                                                                                                     >  2:256761/1‑139 (MQ=255)      gcCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGt                                                                                                   >  2:36344/1‑139 (MQ=255)           aaaaTTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTg                                                                                              <  2:1500682/139‑1 (MQ=255)             aaTTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGGCTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGcc                                                                                            <  1:1366419/139‑1 (MQ=255)                    tCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAg                                                                                     <  1:10345/139‑1 (MQ=255)                    tCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAg                                                                                     <  1:619178/139‑1 (MQ=255)                             ctggGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTgcgc                                                                            <  2:902997/139‑1 (MQ=255)                             ctggGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTgcgc                                                                            <  1:1172077/139‑1 (MQ=255)                              tggGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGca                                                                           <  1:1592214/139‑1 (MQ=255)                                      gCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCg                                                                                                    >  2:1719660/1‑106 (MQ=255)                                      gCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCg                                                                                                    <  1:1719660/106‑1 (MQ=255)                                                aaTGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGaa                                                         <  1:1237450/139‑1 (MQ=255)                                                       ccATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtg                                                  <  1:430861/139‑1 (MQ=255)                                                         aTCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtgtg                                                <  1:36344/139‑1 (MQ=255)                                                           ccACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGt                                              <  1:592151/139‑1 (MQ=255)                                                              cacCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTg                                                                   >  2:296807/1‑115 (MQ=255)                                                              cacCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTg                                                                   <  1:296807/115‑1 (MQ=255)                                                                   ttGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGt                                                                      <  1:1268490/107‑1 (MQ=255)                                                                   ttGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGt                                                                      >  2:1268490/1‑107 (MQ=255)                                                                   ttGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATc                                      <  1:482405/139‑1 (MQ=255)                                                                    tGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAAtt                                                                                >  2:1447519/1‑96 (MQ=255)                                                                    tGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAAtt                                                                                <  1:1447519/96‑1 (MQ=255)                                                                    tGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATcc                                     >  2:216436/1‑139 (MQ=255)                                                                        gcaCGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAGATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTgcg                                 <  1:986382/139‑1 (MQ=255)                                                                        gcaCGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTgcg                                 <  1:313838/139‑1 (MQ=255)                                                                         caCGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtg                                                  >  1:834724/1‑121 (MQ=255)                                                                         caCGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtg                                                  <  2:834724/121‑1 (MQ=255)                                                                           cgacgGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGctc                              >  1:1602551/1‑139 (MQ=255)                                                                             acgGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGt                                                                                                      <  1:1439371/65‑1 (MQ=255)                                                                             acgGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGt                                                                                                      >  2:1439371/1‑65 (MQ=255)                                                                              cgGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTg                                                                   >  2:272906/1‑99 (MQ=255)                                                                              cgGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTg                                                                   <  1:272906/99‑1 (MQ=255)                                                                              cgGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGctctct                           >  2:615986/1‑139 (MQ=255)                                                                                      gCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAgg                   <  1:615986/139‑1 (MQ=255)                                                                                        cccTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCTACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGgcg                 <  2:1071024/139‑1 (MQ=255)                                                                                        cccTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAGATTgcgc                                                                            >  2:1090309/1‑80 (MQ=255)                                                                                        cccTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTgcgc                                                                            <  1:1090309/80‑1 (MQ=255)                                                                                        cccTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtg                                                  >  1:171350/1‑106 (MQ=255)                                                                                        cccTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtg                                                  <  2:171350/106‑1 (MQ=255)                                                                                        cccTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGgcg                 <  2:435448/139‑1 (MQ=255)                                                                                           tGGACTCCAGTTCTTGCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGGTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCgg              >  1:1199112/1‑139 (MQ=255)                                                                                           tGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCgg              >  2:477453/1‑139 (MQ=255)                                                                                                tCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATc                                      >  1:1076737/1‑110 (MQ=255)                                                                                                tCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATc                                      <  2:1076737/110‑1 (MQ=255)                                                                                                  gaGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtgtg                                                >  1:1122714/2‑98 (MQ=255)                                                                                                  cAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACgg                                                                                                        >  2:601489/1‑42 (MQ=255)                                                                                                  cAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACgg                                                                                                        <  1:601489/42‑1 (MQ=255)                                                                                                  cAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGcc                                                                                            >  1:1345561/1‑54 (MQ=255)                                                                                                  cAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGcc                                                                                            <  2:1345561/54‑1 (MQ=255)                                                                                                  cAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGcc                                                                                            >  2:1190054/1‑54 (MQ=255)                                                                                                  cAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGcc                                                                                            <  1:1190054/54‑1 (MQ=255)                                                                                                  cAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGCTTCACGAACTGGTtgtg                                                <  2:1122714/98‑1 (MQ=255)                                                                                                     ttcttcCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGaaa          <  2:1608840/133‑1 (MQ=255)                                                                                                     ttcttcCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGaaa          >  1:1608840/1‑133 (MQ=255)                                                                                                     ttcttcCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCtg    <  1:216436/139‑1 (MQ=255)                                                                                                       cttcCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCtgtg  >  2:892443/1‑139 (MQ=255)                                                                                                       cttcCGCCTGGCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCAGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCtgtg  >  1:10481/1‑139 (MQ=255)                                                                                                                 gCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtgtg                                                >  2:268378/1‑83 (MQ=255)                                                                                                                 gCGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTtgtg                                                <  1:268378/83‑1 (MQ=255)                                                                                                                  cGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGaaa                                                                                  <  2:4493/48‑1 (MQ=255)                                                                                                                  cGGGTCAGGCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGaaa                                                                                  >  1:4493/1‑48 (MQ=255)                                                                                                                          gCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGca                                                                           >  2:1496609/1‑47 (MQ=255)                                                                                                                          gCACAAAATCACCCACGGGTCGTTAGTGCCGACATAGAAATTGCGca                                                                           <  1:1496609/47‑1 (MQ=255)                                                                                                                                        |                                                                                                          GACGCGCCAGAAAATTGTTTCGTTCTGGCTGGGAGTGGCTTTCACGGAATGCCGCCATCCACACCGTTGCAGCACGACGGTATAAGCCCCTGGACTCCAGTTCTTCCGCCTGGCGGGTCAGGCACAAAATCACCCGCGGGTCGTTAGTGCCGACATAGAAATTGCGCACAGGTCTGGTTTCACGAACTGGTTGTGGTTCCGGATCCTGCGCTCTCTCAGTCAGGCGCGGGAAATGTCTGTG  >  NC_000913/2472470‑2472710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑