Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,494,973	G→A	*92* (TAG→TAA)	ypdI →	putative lipoprotein involved in colanic acid biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,494,973	0	G	A	100.0%	282.3 / NA	88	*92* (TAG→TAA)	ypdI	putative lipoprotein involved in colanic acid biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/44);  total (44/44)

ACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAG  >  NC_000913/2494845‑2495106                                                                                                                                  |                                                                                                                                      aCAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATC‑TATTCATCCTGTGCGATAACGTCTTTcaca                                                                                                                            >  1:145124/1‑139 (MQ=255)     aaCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATgg                                                                                                                         >  2:119485/1‑139 (MQ=255)     aaCTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATgg                                                                                                                         <  1:984501/139‑1 (MQ=255)        ttCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                                                      >  1:569958/1‑139 (MQ=255)        ttCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                                                      >  2:1450966/1‑139 (MQ=255)          cAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGt                                                                                                                    >  1:314677/1‑139 (MQ=255)          cAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATC‑TATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTg                                                                                                                   <  2:145124/139‑1 (MQ=255)           aGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTg                                                                                                                   <  1:1450966/139‑1 (MQ=255)            gCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGa                                                                                                                  <  1:72971/139‑1 (MQ=255)            gCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGa                                                                                                                  <  1:584475/139‑1 (MQ=255)             ccATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGaa                                                                                                                 <  2:1292889/139‑1 (MQ=255)                  acaGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATc                                                                                                            <  1:902798/139‑1 (MQ=255)                      gCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGt                                                                                                        >  1:557395/1‑139 (MQ=255)                       cGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTc                                                                                                       <  2:557395/139‑1 (MQ=255)                         aTTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                                                      <  1:1507297/122‑1 (MQ=255)                         aTTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                                                      >  2:1507297/1‑122 (MQ=255)                          ttttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctga                                                                                                    >  2:847598/1‑139 (MQ=255)                          ttttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctga                                                                                                    <  2:569958/139‑1 (MQ=255)                           tttGTTTAAGATGAGAGACGATGCGCCACCAATAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATCCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgat                                                                                                   >  2:1424175/1‑139 (MQ=255)                           tttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgat                                                                                                   >  1:789274/1‑139 (MQ=255)                            ttGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatg                                                                                                  >  1:354861/1‑139 (MQ=255)                                ttAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCCGTGCGATAACGTCTTTCACATGGCAGGTGAACAATc                                                                                                            <  2:1091145/125‑1 (MQ=255)                                ttAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCCGTGCGATAACGTCTTTCACATGGCAGGTGAACAATc                                                                                                            >  1:1091145/1‑125 (MQ=255)                                    gATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgtg                                                                                          >  2:354073/1‑139 (MQ=255)                                         gagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCCTTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAaca                                                                                      >  1:940451/1‑139 (MQ=255)                                         gagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAacac                                                                                     <  2:459656/139‑1 (MQ=255)                                         gagaAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAacac                                                                                     >  1:8551/1‑139 (MQ=255)                                              gaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGcc                                                                                <  1:354073/139‑1 (MQ=255)                                              gaTGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGcc                                                                                >  1:1032948/1‑139 (MQ=255)                                               atgggcCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGccc                                                                               >  1:935367/5‑139 (MQ=255)                                                      accaTTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACACAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg                                                                        <  1:977616/139‑1 (MQ=255)                                                      accaTTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATc                                                                                                            <  2:599301/103‑1 (MQ=255)                                                      accaTTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATc                                                                                                            >  1:599301/1‑103 (MQ=255)                                                      accaTTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAg                                                                        <  1:847598/139‑1 (MQ=255)                                                             aaCCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCt                                                                 >  1:885712/1‑139 (MQ=255)                                                               ccTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGa                                                               <  1:119485/139‑1 (MQ=255)                                                                  cacaGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGTGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAac                                                                                       <  2:1576087/112‑1 (MQ=255)                                                                  cacaGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGTGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAac                                                                                       >  1:1576087/1‑112 (MQ=255)                                                                  cacaGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                                                      <  2:1565718/81‑1 (MQ=255)                                                                  cacaGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                                                      >  1:1565718/1‑81 (MQ=255)                                                                  cacaGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctg                                                                                                     >  2:1712364/1‑98 (MQ=255)                                                                  cacaGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctg                                                                                                     <  1:1712364/98‑1 (MQ=255)                                                                  cacaGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGAccc                                                            >  1:824155/1‑139 (MQ=255)                                                                    caGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCat                                                          >  2:862455/1‑138 (MQ=255)                                                                        agaaATGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTAcc                                                      <  2:1403152/139‑1 (MQ=255)                                                                           aaTGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGcc                                                   <  2:8551/139‑1 (MQ=255)                                                                            aTGCCGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATAGCAGGTGAACAATCATGTctgatg                                                                                                  <  1:1219750/91‑1 (MQ=255)                                                                            aTGACGCCCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatg                                                                                                  >  2:1219750/1‑91 (MQ=255)                                                                             ttccgccCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCatta                                                        <  2:1069935/130‑4 (MQ=255)                                                                             tGCCGCCCCC‑TTTTCCGACAAAGGCGAGTCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACGCATTa                                                        >  1:1069935/1‑132 (MQ=255)                                                                                cgccCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCa                                              <  2:314677/139‑1 (MQ=255)                                                                                cgccCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCa                                              <  1:1491491/139‑1 (MQ=255)                                                                                 gccCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCAt                                             >  1:957516/1‑139 (MQ=255)                                                                                 gccCCC‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCAt                                             >  2:1211481/1‑139 (MQ=255)                                                                                  ccccc‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATa                                            <  1:862455/139‑1 (MQ=255)                                                                                    ccc‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCa                                              >  2:388015/1‑135 (MQ=255)                                                                                    ccc‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCa                                              <  1:388015/135‑1 (MQ=255)                                                                                     cctttttCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATAcc                                          <  2:940451/136‑1 (MQ=255)                                                                                     cc‑TTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATAccc                                         <  2:789274/139‑1 (MQ=255)                                                                                           tCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCccacca                                    <  2:824155/139‑1 (MQ=255)                                                                                             cGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATa                                  <  1:1677256/139‑1 (MQ=255)                                                                                             cGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATa                                  >  1:1642059/1‑139 (MQ=255)                                                                                              gACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                                                      <  1:868116/54‑1 (MQ=39)                                                                                              gACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                                                      >  2:868116/1‑54 (MQ=38)                                                                                               aCAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgtg                                                                                          >  1:655717/1‑81 (MQ=255)                                                                                               aCAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGAtgtg                                                                                          <  2:655717/81‑1 (MQ=255)                                                                                                   aGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGa                            <  1:368637/139‑1 (MQ=255)                                                                                                         atcaTCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                                                      >  1:404970/4‑43 (MQ=38)                                                                                                         atcaTCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAg                                                                                                                      <  2:404970/40‑1 (MQ=37)                                                                                                         atcaTCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAatcatc                      <  2:935367/136‑1 (MQ=255)                                                                                                          tcaTCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAAtcatca                     <  1:1424175/137‑1 (MQ=255)                                                                                                                       ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCTAATCATCAAGTCACAAGCCgg        >  2:483959/1‑139 (MQ=255)                                                                                                                       ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGcc          <  1:1651074/137‑1 (MQ=255)                                                                                                                       ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGcc          >  2:1651074/1‑137 (MQ=255)                                                                                                                       ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCgg        >  1:959285/1‑139 (MQ=255)                                                                                                                       ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCgg        >  1:1627710/1‑139 (MQ=255)                                                                                                                       ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCACAAAGCGAATCATCAAGCCACAAGCCgg        >  2:1375537/1‑139 (MQ=255)                                                                                                                           tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctg                                                                                               <  1:1273979/48‑1 (MQ=255)                                                                                                                           tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatgctg                                                                                               >  2:1273979/1‑48 (MQ=255)                                                                                                                           tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAacac                                                                                     >  2:574704/1‑58 (MQ=255)                                                                                                                           tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAacac                                                                                     <  1:574704/58‑1 (MQ=255)                                                                                                                           tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTg                                                 <  2:682376/94‑1 (MQ=255)                                                                                                                           tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTg                                                 >  1:682376/1‑94 (MQ=255)                                                                                                                           tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGcc                 <  2:1086186/126‑1 (MQ=255)                                                                                                                           tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGcc                 >  1:1086186/1‑126 (MQ=255)                                                                                                                            gCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatg                                                                                                  >  2:1290537/1‑44 (MQ=255)                                                                                                                            gCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTctgatg                                                                                                  <  1:1290537/44‑1 (MQ=255)                                                                                                                             cGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAg  >  2:344024/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                      ACAAAACTTCAGCCATCACAGGCGATTTTGTTTAAGATGAGAGAAGATGCGCCACCATTAAACCTCACAGAAGAAATGCCGCCCCC‑TTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAG  >  NC_000913/2494845‑2495106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑