Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,574,135	G→A	D57D (GAC→GAT)	eutT ←	cobalamin adenosyltransferase involved in ethanolamine utilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,574,135	0	G	A	100.0%	141.8 / NA	44	D57D (GAC→GAT)	eutT	cobalamin adenosyltransferase involved in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/17);  total (27/17)

CCATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTT  >  NC_000913/2573999‑2574269                                                                                                                                         |                                                                                                                                       ccATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCatcatc                                                                                                                                      >  1:232901/1‑139 (MQ=255)           cgccgACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAgg                                                                                                                            <  2:1608911/139‑1 (MQ=255)             ccgACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAggcg                                                                                                                          >  2:882897/1‑139 (MQ=255)                            gCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAAt                                                                                                           >  2:1612463/1‑139 (MQ=255)                             cGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATa                                                                                                          >  2:1355038/1‑139 (MQ=255)                                 tCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACt                                                                                                      >  1:1400846/1‑139 (MQ=255)                                   cGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGACTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTg                                                                                                    >  1:1440840/1‑139 (MQ=255)                                                         cGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCa                                                                              >  2:381406/1‑139 (MQ=255)                                                             acaGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCaa                                                                          <  2:1400846/139‑1 (MQ=255)                                                                 ttCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGGGCAGATGGCGGCTTTCCAGCAACTcc                                                                      >  1:1542118/1‑139 (MQ=255)                                                                      agcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGc                                                                 >  2:1596275/1‑139 (MQ=255)                                                                         aCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCaga                                                              >  1:365014/1‑139 (MQ=255)                                                                                cGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTc                                                       >  2:146794/1‑139 (MQ=255)                                                                                   aTGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAgg                                                    <  2:365014/139‑1 (MQ=255)                                                                                          tCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGc                                                                            >  1:1494946/1‑108 (MQ=255)                                                                                          tCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGc                                                                            <  2:1494946/108‑1 (MQ=255)                                                                                           cGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTc                                            >  1:1132085/1‑139 (MQ=255)                                                                                             cTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAAc                                                                         >  2:357596/1‑108 (MQ=255)                                                                                             cTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAAc                                                                         <  1:357596/108‑1 (MQ=255)                                                                                               aCTGGTCAGCCCATGAAAGGGCTGCGGCTGCTGCTGTGCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCCACAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCa                                        >  1:1182028/1‑139 (MQ=255)                                                                                                cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg                                       >  2:744935/1‑139 (MQ=255)                                                                                                cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg                                       <  1:744935/139‑1 (MQ=255)                                                                                                cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg                                       <  2:232901/139‑1 (MQ=255)                                                                                                cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAg                                       <  2:1132085/139‑1 (MQ=255)                                                                                                 tGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGGGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAgg                                      <  2:1542118/139‑1 (MQ=255)                                                                                                 tGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAgg                                      >  1:433979/1‑139 (MQ=255)                                                                                                        cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaacaa                                                                                                                                 >  2:1716836/1‑41 (MQ=255)                                                                                                        cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaacaa                                                                                                                                 <  1:1716836/41‑1 (MQ=255)                                                                                                          cATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGAt                             <  1:1340301/139‑1 (MQ=255)                                                                                                             gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATACACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACt                                               >  2:1334053/1‑118 (MQ=255)                                                                                                             gAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACt                                               <  1:1334053/118‑1 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACt                                                                                                      <  1:1004091/58‑1 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACt                                                                                                      >  2:1004091/1‑58 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATgg                                                                                        <  1:214401/72‑1 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATgg                                                                                        >  2:214401/1‑72 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACt                                               >  1:875473/1‑113 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACt                                               <  2:875473/113‑1 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTgcgc                     >  1:627414/1‑139 (MQ=255)                                                                                                                   actgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGcc                    <  2:1440840/138‑1 (MQ=255)                                                                                                                     tgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCtt                  >  2:1395727/1‑139 (MQ=255)                                                                                                                     tgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCtt                  >  2:6456/1‑139 (MQ=255)                                                                                                                       cggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCg                >  1:600769/1‑139 (MQ=255)                                                                                                                             ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATgg                               >  1:560105/1‑118 (MQ=255)                                                                                                                             ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATgg                               <  2:560105/118‑1 (MQ=255)                                                                                                                             ctgctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGc          >  1:633323/1‑139 (MQ=255)                                                                                                                                    tcatcatcaACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGc                                                                            <  1:574560/66‑1 (MQ=255)                                                                                                                                    tcatcatcaACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGc                                                                            >  2:574560/1‑66 (MQ=255)                                                                                                                                     catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt  <  1:715403/139‑1 (MQ=255)                                                                                                                                     catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt  <  1:146794/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CCATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTT  >  NC_000913/2573999‑2574269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑