| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,608,367 | T→C | I484T (ATC→ACC) | hyfF → | hydrogenase 4, membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,608,367 | 0 | T | C | 98.5% | 230.3 / NA | 65 | I484T (ATC→ACC) | hyfF | hydrogenase 4, membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (31/33); minor base G (1/0); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCA > NC_000913/2608232‑2608501 | tGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAg > 1:1730533/1‑139 (MQ=255) cTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGAt > 2:522794/1‑139 (MQ=255) tGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATc > 1:441909/1‑139 (MQ=255) cgGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTgg > 1:618552/1‑139 (MQ=255) tgtTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggtgac < 2:1125320/139‑5 (MQ=255) tgtTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcggg < 1:33416/139‑1 (MQ=255) gtTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcgggc < 1:522794/139‑1 (MQ=255) tAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGgcgc < 2:1503596/139‑1 (MQ=255) aTGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCtt < 2:1747262/139‑1 (MQ=255) aTGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCtt < 1:1504847/139‑1 (MQ=255) tGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTc < 2:618552/139‑1 (MQ=255) tGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGCGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTc < 1:1132688/139‑1 (MQ=255) cGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCAc > 2:998945/1‑139 (MQ=255) aaCCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACtat > 2:1464696/1‑139 (MQ=255) cgccgcAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGt < 1:1640152/139‑1 (MQ=255) cgccgcAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGt < 2:1730533/139‑1 (MQ=255) cgcAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGt > 1:694801/1‑136 (MQ=255) cgcAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGt < 2:694801/136‑1 (MQ=255) cgcAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCct > 1:487224/1‑139 (MQ=255) ccGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAgg < 2:441909/139‑1 (MQ=255) gTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGa < 1:106414/139‑1 (MQ=255) ttAACCGGGGTGATCTCGACTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTc > 2:933467/1‑118 (MQ=255) ttAACCGGGGTGATCTCGACTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTc < 1:933467/118‑1 (MQ=255) ggggTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTc > 1:1387600/1‑112 (MQ=255) ggggTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTc < 2:1387600/112‑1 (MQ=255) ggTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATc > 2:599755/1‑139 (MQ=255) ggTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATc > 1:1109636/1‑139 (MQ=255) gTGATCTCAGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATct < 1:1093110/139‑1 (MQ=255) gATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATctgc < 1:1464696/139‑1 (MQ=255) tctcGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcgggc < 2:807415/100‑1 (MQ=255) tctcGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcgggc > 1:807415/1‑100 (MQ=255) gTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcggg > 2:1555737/1‑90 (MQ=255) gTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTggcggg < 1:1555737/90‑1 (MQ=255) ttGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTa > 2:619335/1‑139 (MQ=255) tCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCt > 1:61619/1‑139 (MQ=255) tgGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCAGCAGGATCCTGGCGGGCGCTTCCACt > 1:1036490/1‑71 (MQ=255) tgGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACt < 2:1036490/71‑1 (MQ=255) tgGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAgg < 1:998945/139‑1 (MQ=255) gTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAgg > 1:84612/1‑83 (MQ=255) gTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAgg < 2:84612/83‑1 (MQ=255) gTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCa > 1:764603/1‑139 (MQ=255) tCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCAc < 1:916057/139‑1 (MQ=255) atgatgCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGgcgc > 2:1285664/1‑59 (MQ=255) atgatgCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGgcgc < 1:1285664/59‑1 (MQ=255) atgCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAGCGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCAt < 2:1713023/139‑1 (MQ=255) atgCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCAt < 1:932284/139‑1 (MQ=255) atgCTGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCAt < 2:61619/139‑1 (MQ=255) tGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTg < 1:566123/119‑1 (MQ=255) tGGCGATGGGAACGCATATTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTg > 2:566123/1‑119 (MQ=255) atTCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTg < 2:764603/139‑1 (MQ=255) tCCACAACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGaa < 1:1250528/139‑1 (MQ=255) caACCTGTCACCAGGATCCTGGCGGGCGCTTCCACtat > 2:1700964/1‑38 (MQ=255) caACCTGTCACCAGGATCCTGGCGGGCGCTTCCACtat < 1:1700964/38‑1 (MQ=255) caACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACg < 2:584315/74‑1 (MQ=255) caACCTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACg > 1:584315/1‑74 (MQ=255) ccTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGAttttt < 2:1639732/91‑1 (MQ=255) ccTGTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGAttttt > 1:1639732/1‑91 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCa > 1:1186589/1‑139 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCa > 2:412403/1‑139 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCa > 2:1650338/1‑139 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCa > 2:1338966/1‑139 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCa > 2:83185/1‑139 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCa > 1:256597/1‑139 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCa > 2:966165/1‑139 (MQ=255) gTCACCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCa > 1:436536/1‑139 (MQ=255) | TGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCA > NC_000913/2608232‑2608501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |