Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,724,448	C→T	H107H (CAC→CAT)  *433* (TAG→TAA)	yfiM → kgtP ←	putative lipoprotein alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,724,448	0	C	T	100.0%	137.9 / NA	43	H107H (CAC→CAT)  *433* (TAG→TAA)	yfiM kgtP	putative lipoprotein alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/22);  total (21/22)

TGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGC  >  NC_000913/2724312‑2724582                                                                                                                                         |                                                                                                                                       tGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTa                                                                                                                                      >  1:1502587/1‑139 (MQ=255)     ctctGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGa                                                                                                                                  >  2:1764545/1‑139 (MQ=255)     ctctGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCAACGGCTATACCATCTGGCAACTGACCCGTCATTAAAGa                                                                                                                                  >  1:1145199/1‑139 (MQ=255)        tGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                               <  2:1647849/139‑1 (MQ=255)        tGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                               <  2:1502587/139‑1 (MQ=255)             gTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATccc                                                                                                                          <  1:618235/139‑1 (MQ=255)               ttGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCccct                                                                                                                        <  2:1377380/139‑1 (MQ=255)                    ggCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCcccttccct                                                                                                                   <  2:373606/139‑1 (MQ=255)                      cGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCttt                                                                                                                 >  2:397727/1‑139 (MQ=255)                       gTCAAAAGAGCTTTGGGATAGTCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGGCCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                                <  2:1447284/139‑1 (MQ=255)                        tCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGAc                                                                                                                                 <  2:409085/121‑1 (MQ=255)                        tCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGAc                                                                                                                                 >  1:409085/1‑121 (MQ=255)                             agagCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGt                                                                                                          <  1:1494317/139‑1 (MQ=255)                                                cccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCccct                                                                                                                        >  1:1356933/1‑106 (MQ=255)                                                cccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCccct                                                                                                                        <  2:1356933/106‑1 (MQ=255)                                                 ccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGTAAGCACCGGCTATACCGTCTGGCTACTGACCCGTCATTATAGACGCATCCTCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGaaa                                                                                      <  2:1145199/139‑1 (MQ=255)                                                         aGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCcccttcc                                                                                                                     <  2:107176/100‑1 (MQ=255)                                                         aGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCcccttcc                                                                                                                     >  1:107176/1‑100 (MQ=255)                                                         aGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGccacca                                                                              <  1:397727/139‑1 (MQ=255)                                                         aGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGccacca                                                                              >  2:193500/1‑139 (MQ=255)                                                              ctggagctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGcc                                                                         >  2:267296/1‑139 (MQ=255)                                                                      ggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGt                                                                 <  1:1764545/139‑1 (MQ=255)                                                                       gaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTc                                                                >  2:495449/1‑139 (MQ=255)                                                                                        aTGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGc                                               <  1:193500/139‑1 (MQ=255)                                                                                           tCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATg                                                                                                           >  2:910205/1‑76 (MQ=255)                                                                                           tCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATg                                                                                                           <  1:910205/76‑1 (MQ=255)                                                                                           tCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGt                                            >  1:547708/1‑139 (MQ=255)                                                                                              ccGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTc                                         <  1:1603879/139‑1 (MQ=255)                                                                                                ggTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCa                                       <  2:547708/139‑1 (MQ=255)                                                                                                       gCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAt                                >  1:629861/1‑139 (MQ=255)                                                                                                           cGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGAt                            >  2:1786673/1‑139 (MQ=255)                                                                                                            ggCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTcc                                        <  1:84250/126‑1 (MQ=255)                                                                                                            ggCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTcc                                        >  2:84250/1‑126 (MQ=255)                                                                                                                   ccGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGTATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGa                    <  2:955945/139‑1 (MQ=255)                                                                                                                   ccGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGa                    <  1:267296/139‑1 (MQ=255)                                                                                                                          gCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCt             >  1:1655518/1‑139 (MQ=255)                                                                                                                          gCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCt             >  1:202366/1‑139 (MQ=255)                                                                                                                             aCTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACg          >  1:52909/1‑139 (MQ=255)                                                                                                                              cTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGt         >  2:1229181/1‑139 (MQ=255)                                                                                                                                 aCCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACt      >  1:1467681/1‑139 (MQ=255)                                                                                                                                    cGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGaa                                                                                       >  2:65568/1‑55 (MQ=255)                                                                                                                                    cGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGaa                                                                                       <  1:65568/55‑1 (MQ=255)                                                                                                                                     gTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTccgc  <  2:52909/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TGTTCTCTGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCGC  >  NC_000913/2724312‑2724582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑