Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,893,928 G→A *424* (TAG→TAA
A4T (GCC→ACC) 
ygcN →
ygcO →
putative oxidoreductase
putative 4Fe‑4S cluster‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,893,9280GA100.0% 198.9 / NA 62*424* (TAG→TAA
A4T (GCC→ACC) 
ygcN
ygcO
putative oxidoreductase
putative 4Fe‑4S cluster‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/31);  total (31/31)

GGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTAC  >  NC_000913/2893793‑2894060
                                                                                                                                       |                                                                                                                                    
ggATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAAccc                                                                                                                                   >  1:511426/1‑139 (MQ=255)
         ccGGGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCAGGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATctct                                                                                                                          >  1:1156043/1‑139 (MQ=255)
            cgATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGgc                                                                                                                       >  2:1381148/1‑139 (MQ=255)
             gATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGgcg                                                                                                                      <  2:1156043/139‑1 (MQ=255)
             gATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGgcg                                                                                                                      >  2:49557/1‑139 (MQ=255)
               tttATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGcgcg                                                                                                                    <  1:1249183/139‑1 (MQ=255)
                            ggTGATAAACCTGTTCCACCGATGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCTTCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGcgcc                                                                                                       >  2:425858/1‑139 (MQ=255)
                               gATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGat                                                                                                    >  1:379384‑M1/1‑137 (MQ=255)
                                   aaCCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt                                                                                                <  2:379384‑M1/139‑7 (MQ=255)
                                             aCCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgact                                                                                      <  2:1691633‑M1/139‑17 (MQ=255)
                                                gCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccg                                                                                   >  2:110613‑M1/1‑120 (MQ=255)
                                                gCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccg                                                                                   >  2:1110739‑M1/1‑120 (MQ=255)
                                                         gTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggc                                                                                           <  1:271590‑M1/122‑12 (MQ=255)
                                                         gTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggc                                                                                           >  2:271590‑M1/1‑111 (MQ=255)
                                                          ttATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgcca                                                                         >  2:1682832‑M1/1‑110 (MQ=255)
                                                                 ggCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggca                                                                  <  1:1214316‑M1/139‑37 (MQ=255)
                                                                  gCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcag                                                                 <  1:1261330‑M1/139‑38 (MQ=255)
                                                                    atcatTTACGTCTTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa                                                               <  1:425858‑M1/139‑40 (MQ=255)
                                                                    atcatTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATg                                                                                                           >  1:25937/1‑95 (MQ=255)
                                                                    atcatTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATg                                                                                                           <  2:25937/95‑1 (MQ=255)
                                                                               cgtcACGGCCCGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTgg                                                                                                                        >  2:139206/1‑71 (MQ=255)
                                                                               cgtcACGGCCCGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTgg                                                                                                                        <  1:139206/71‑1 (MQ=255)
                                                                                gtcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaac                                                   >  1:784262‑M1/1‑88 (MQ=255)
                                                                                gtcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaac                                                   >  2:916499‑M1/1‑88 (MQ=255)
                                                                                gtcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaac                                                   >  2:931277‑M1/1‑88 (MQ=255)
                                                                                 tcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt                                                                                                >  1:129422‑M1/1‑87 (MQ=255)
                                                                                 tcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt                                                                                                <  2:129422‑M1/93‑7 (MQ=255)
                                                                                           tgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggc                                        <  1:983770‑M1/139‑63 (MQ=255)
                                                                                           tgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggc                                        >  2:983770‑M1/1‑77 (MQ=255)
                                                                                           tgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctg                                              <  1:123796‑M1/133‑57 (MQ=255)
                                                                                           tgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctg                                              >  2:123796‑M1/1‑77 (MQ=255)
                                                                                                 cTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGt                                                                                                                                 >  1:1096299/1‑44 (MQ=255)
                                                                                                 cTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGt                                                                                                                                 <  2:1096299/44‑1 (MQ=255)
                                                                                                     cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAAtctc                                                                                                                           <  1:1524864/46‑1 (MQ=255)
                                                                                                     cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAAtctc                                                                                                                           >  2:1524864/1‑46 (MQ=255)
                                                                                                     cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttt                              <  2:355972‑M1/139‑73 (MQ=255)
                                                                                                            tGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctca                       <  1:916499‑M1/139‑80 (MQ=255)
                                                                                                               tATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacac                    <  1:363069‑M1/139‑83 (MQ=255)
                                                                                                               tATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacac                    <  2:1362996‑M1/139‑83 (MQ=255)
                                                                                                                 tCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccg                  <  2:784262‑M1/139‑85 (MQ=255)
                                                                                                                 tCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGactgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccg                  <  2:891140‑M1/139‑85 (MQ=255)
                                                                                                                  cAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgg                 <  1:1110739‑M1/139‑86 (MQ=255)
                                                                                                                  cAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgg                 <  1:1381148‑M1/139‑86 (MQ=255)
                                                                                                                      aGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaagg             >  1:369166‑M1/1‑50 (MQ=255)
                                                                                                                      aGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgtt                                                                                 >  1:8022‑M1/1‑50 (MQ=255)
                                                                                                                      aGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgtt                                                                                 <  2:8022‑M1/71‑22 (MQ=255)
                                                                                                                       gTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggt            <  1:49557‑M1/139‑91 (MQ=255)
                                                                                                                       gTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggt            <  1:110613‑M1/139‑91 (MQ=255)
                                                                                                                        tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggta           >  1:753157‑M1/1‑48 (MQ=255)
                                                                                                                        tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggta           >  2:1224815‑M1/1‑48 (MQ=255)
                                                                                                                        tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggta           >  1:1600815‑M1/1‑48 (MQ=255)
                                                                                                                        tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgga                >  1:608041‑M1/1‑48 (MQ=255)
                                                                                                                        tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgga                <  2:608041‑M1/134‑87 (MQ=255)
                                                                                                                        tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccaga                                                                       <  1:1037042‑M1/79‑32 (MQ=255)
                                                                                                                        tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccaga                                                                       >  2:1037042‑M1/1‑48 (MQ=255)
                                                                                                                          tGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaac         >  2:554762‑M1/1‑46 (MQ=255)
                                                                                                                          tGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgag                                                                              >  1:962615‑M1/1‑46 (MQ=255)
                                                                                                                          tGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgag                                                                              <  2:962615‑M1/70‑25 (MQ=255)
                                                                                                                            cGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaactt       <  2:753157‑M1/139‑96 (MQ=255)
                                                                                                                                 tCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaacttacgta  >  1:986432‑M1/1‑39 (MQ=255)
                                                                                                                                 tCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgct                         <  1:1757307‑M1/116‑78 (MQ=255)
                                                                                                                                 tCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgct                         >  2:1757307‑M1/1‑39 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTAC  >  NC_000913/2893793‑2894060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: