Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,915,105	C→T	P17S (CCG→TCG)	barA →	hybrid sensory histidine kinase, in two‑component regulatory system with UvrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,915,105	0	C	T	100.0%	235.1 / NA	72	P17S (CCG→TCG)	barA	hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/38);  total (34/38)

TGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCACCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGA  >  NC_000913/2914970‑2915237                                                                                                                                        |                                                                                                                                     tGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcga                                                                                                                                   <  2:1251932/139‑1 (MQ=255)   cgtcgtTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                 <  2:383420/139‑1 (MQ=255)   cgtcgtTTTGCAGAGTAGAATTGCGCCATTGGCGAGACCTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                 <  1:1629263/139‑1 (MQ=255)       gtTTTGCAGAGTAGAATCGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTcc                                                                                                                             <  2:27561/139‑1 (MQ=255)                  tAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTa                                                                                                                  >  1:557496/1‑139 (MQ=255)                     aaTTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTg                                                                                                               <  1:1208762/139‑1 (MQ=255)                       ttGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTg                                                                                                               <  1:1558575/137‑1 (MQ=255)                       ttGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTg                                                                                                               >  2:1558575/1‑137 (MQ=255)                          cgcCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAg                                                                                                          >  1:929991/1‑139 (MQ=255)                           gcCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGt                                                                                                         <  2:1737398/139‑1 (MQ=255)                            ccATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTa                                                                                                        >  2:713412/1‑139 (MQ=255)                                  gCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCtttt                                                                                                  <  2:277895/139‑1 (MQ=255)                                     agaCTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTtcg                                                                                               <  2:557496/139‑1 (MQ=255)                                            ctcAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAg                                                                                                          >  2:195263/1‑121 (MQ=255)                                            ctcAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAg                                                                                                          <  1:195263/121‑1 (MQ=255)                                            ctcAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCa                                                                                        >  2:206242/1‑139 (MQ=255)                                             tcAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       <  1:467224/139‑1 (MQ=255)                                                           tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACtt                                                                         >  1:1354873/1‑139 (MQ=255)                                                                 tAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGTTATAACGACTTGCAGCg                                                                   <  1:634955/139‑1 (MQ=255)                                                                 tAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCg                                                                   >  2:45004/1‑139 (MQ=255)                                                                 tAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCg                                                                   <  2:1080762/139‑1 (MQ=255)                                                                   aTTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATAGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTc                                                                 >  2:1071464/1‑139 (MQ=255)                                                                       tCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgac                                                                                                                                  >  2:725326/1‑70 (MQ=255)                                                                       tCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgac                                                                                                                                  <  1:725326/70‑1 (MQ=255)                                                                       tCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACt                                                             >  2:961351/1‑139 (MQ=255)                                                                                ggAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGc                                                    <  2:1354873/139‑1 (MQ=255)                                                                                 gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCt                                                                                                             >  1:1722413/1‑81 (MQ=255)                                                                                 gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCt                                                                                                             <  2:1722413/81‑1 (MQ=255)                                                                                      ccATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGc                                              <  1:206242/139‑1 (MQ=255)                                                                                       cATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       >  2:913076/1‑97 (MQ=255)                                                                                       cATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       <  1:913076/97‑1 (MQ=255)                                                                                         tGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAg                                           <  1:961351/139‑1 (MQ=255)                                                                                            ccAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcga                                                                                                                                   <  2:1380954/48‑1 (MQ=255)                                                                                            ccAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcga                                                                                                                                   >  1:1380954/1‑48 (MQ=255)                                                                                               aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                         <  1:626752/55‑1 (MQ=255)                                                                                               aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                         >  2:626752/1‑55 (MQ=255)                                                                                               aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCattat                                     >  2:328679/1‑139 (MQ=255)                                                                                                 tACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTg                                   >  2:1709246/1‑139 (MQ=255)                                                                                                  aCAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGa                                  <  1:328679/139‑1 (MQ=255)                                                                                                   cAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAg                                 <  2:929991/139‑1 (MQ=255)                                                                                                      ccTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                         <  1:1618473/48‑1 (MQ=255)                                                                                                      ccTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                         >  2:1618473/1‑48 (MQ=255)                                                                                                      ccTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAg                                                                     <  2:871843/100‑1 (MQ=255)                                                                                                      ccTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAg                                                                     >  1:871843/1‑100 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                         >  2:545752/1‑44 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTAt                                                                                                                         <  1:545752/44‑1 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGtt                                                                                                                    >  1:1555115/1‑49 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGtt                                                                                                                    <  2:184748/49‑1 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGtt                                                                                                                    <  2:1555115/49‑1 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGtt                                                                                                                    >  1:184748/1‑49 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTAtt                                                                                                                <  2:503784/53‑1 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTAtt                                                                                                                >  1:503784/1‑53 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       >  2:1630018/1‑78 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       <  1:1630018/78‑1 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCa                                                                      >  1:611702/1‑95 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCa                                                                      <  2:611702/95‑1 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCtt                           >  2:143770/1‑138 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCtt                           <  1:143770/138‑1 (MQ=255)                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTg                          >  1:261464/1‑139 (MQ=255)                                                                                                           gcgcACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                 >  1:653550/1‑35 (MQ=255)                                                                                                           gcgcACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                 <  2:653550/35‑1 (MQ=255)                                                                                                              cacgcaTGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGt                      <  1:1709246/139‑1 (MQ=255)                                                                                                                   atgatgATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTa                 <  2:980493/139‑1 (MQ=255)                                                                                                                    tgatgaTTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTAc                <  1:45004/139‑1 (MQ=255)                                                                                                                           tCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATGACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCa                                         >  1:1256449/1‑107 (MQ=255)                                                                                                                           tCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATGACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCa                                         <  2:1256449/107‑1 (MQ=255)                                                                                                                           tCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAatat         >  1:779654/1‑139 (MQ=255)                                                                                                                               aTCCTGGCATCGACCGTCCTTATTGGTTTATTGTTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCa     >  1:1663178/1‑139 (MQ=255)                                                                                                                               aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       >  2:1738510/1‑57 (MQ=255)                                                                                                                               aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                       <  1:1738510/57‑1 (MQ=255)                                                                                                                               aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGc                                                                    >  2:712392/1‑76 (MQ=255)                                                                                                                               aTCCTGGCATCGACCGTCCGTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGc                                                                    <  1:712392/76‑1 (MQ=255)                                                                                                                                  cTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGa  <  2:1194890/139‑1 (MQ=255)                                                                                                                                   tGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGc                                                                    <  2:1185265/72‑1 (MQ=255)                                                                                                                                   tGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGc                                                                    >  1:1185265/1‑72 (MQ=255)                                                                                                                                   tGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAg                                 >  2:269538/1‑107 (MQ=255)                                                                                                                                   tGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAg                                 <  1:269538/107‑1 (MQ=255)                                                                                                                                    ggCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGt                                                                  >  1:990080/1‑73 (MQ=255)                                                                                                                                    ggCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGt                                                                  <  2:990080/73‑1 (MQ=255)                                                                                                                                     gCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCa     >  1:1731388/1‑133 (MQ=255)                                                                                                                                     gCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCa     <  2:1731388/133‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCACCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATGA  >  NC_000913/2914970‑2915237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑