Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,966,188 | C→T | *749* (TAG→TAA) | ptsP ← | PEP‑protein phosphotransferase enzyme I, GAF domain containing protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,966,188 | 0 | C | T | 100.0% | 132.2 / NA | 42 | *749* (TAG→TAA) | ptsP | PEP‑protein phosphotransferase enzyme I, GAF domain containing protein |
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/14); total (28/14) |
TTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATGCGC > NC_000913/2966057‑2966323 | ttATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAAcc < 1:1245458/139‑1 (MQ=255) gCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCa > 2:1665679/1‑139 (MQ=255) aTTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGc > 1:1143661/1‑139 (MQ=255) aaTGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGa < 1:1665679/139‑1 (MQ=255) cgccgcAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGAc > 2:1421589/1‑134 (MQ=255) cgccgcAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGAc < 1:1421589/134‑1 (MQ=255) cgccgcAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTcc > 1:1079977/1‑139 (MQ=255) cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAgcc > 1:753328/1‑90 (MQ=255) cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAgcc < 2:753328/90‑1 (MQ=255) cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTc > 2:1310267/1‑139 (MQ=255) caATGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAg > 2:1222859/1‑76 (MQ=255) caAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAg < 1:1222859/76‑1 (MQ=255) caAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTg > 2:312621/1‑108 (MQ=255) caAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTg < 1:312621/108‑1 (MQ=255) caAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTg > 1:344844/1‑139 (MQ=255) ggggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATaa < 2:1130563/99‑1 (MQ=255) ggggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATaa > 1:1130563/1‑99 (MQ=255) ggggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTg < 1:904901/112‑1 (MQ=255) ggggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTg > 2:904901/1‑112 (MQ=255) ggggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTgcgc > 1:971903/1‑139 (MQ=255) ggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCtt < 2:221376/139‑1 (MQ=255) ggctggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTc > 2:263917/1‑120 (MQ=255) ggctggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTc < 1:263917/120‑1 (MQ=255) ggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGa < 1:80461/77‑1 (MQ=255) ggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGa > 2:80461/1‑77 (MQ=255) ttGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATGACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCt > 1:137637/1‑139 (MQ=255) ttGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCt > 2:1766376/1‑139 (MQ=255) ggATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCa > 2:153501/1‑139 (MQ=255) tatGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGAc < 1:985822/55‑1 (MQ=255) tatGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGAtttt > 1:1469435/1‑130 (MQ=255) tatGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGAtttt < 2:1469435/130‑1 (MQ=255) tatGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCgg > 1:1295525/1‑139 (MQ=255) tatGTATATGATCCGCGTTATAACCCCCCGCGAATCAGCCCGCCCATGCCGCGAc > 2:985822/1‑55 (MQ=255) atGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGc > 1:1783475/1‑139 (MQ=255) tGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCa > 2:22461/1‑139 (MQ=255) tGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCa > 1:1552450/1‑139 (MQ=255) gATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCaa > 2:145241/1‑139 (MQ=255) aTCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCtt > 1:644421/1‑126 (MQ=255) aTCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCtt < 2:644421/126‑1 (MQ=255) aTCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAAt > 1:1012809/1‑139 (MQ=255) tCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATg > 1:1077231/1‑139 (MQ=255) cgcgTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgcg > 1:567564/1‑139 (MQ=255) gcgtTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCt > 2:1053653/1‑121 (MQ=255) gcgtTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCt < 1:1053653/121‑1 (MQ=255) gcgtTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgcgc < 2:1077231/139‑1 (MQ=255) gcgtTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgcgc > 2:1026207/1‑139 (MQ=255) | TTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATGCGC > NC_000913/2966057‑2966323 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |