Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,411	C→T	*394* (TAG→TAA)	yqeF ←	short chain acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,411	0	C	T	100.0%	207.7 / NA	65	*394* (TAG→TAA)	yqeF	short chain acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/30);  total (35/30)

GTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATC  >  NC_000913/2984276‑2984546                                                                                                                                        |                                                                                                                                        gTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTAt                                                                                                                                      >  2:1070555/1‑139 (MQ=255)            aTCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTc                                                                                                                           >  1:1276077/1‑139 (MQ=255)                   atatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTc                                                                                                                    >  2:1035338/1‑139 (MQ=255)                   atatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTc                                                                                                                    >  2:1751132/1‑139 (MQ=255)                    tatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCa                                                                                                                   <  1:1070555/139‑1 (MQ=255)                       gTCATGAGAATGGAAAAATGAGGGAGCACAGAATGTGTATGTCGACTTGCGAGCCGGCATTATTCAACAATTTACTGATGGCAGATATTACTCGGCAAGGAGGAAATACCGAGTTATTCGTCACGTTCAATGATAAATg                                                                                                                >  1:44665/1‑139 (MQ=255)                               aaTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACAccc                                                                                                        >  1:1181600/1‑139 (MQ=255)                                  ggAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTgg                                                                                                     >  2:208063/1‑139 (MQ=255)                                  ggAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTgg                                                                                                     >  1:438539/1‑139 (MQ=255)                                  ggAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTgg                                                                                                     >  2:288719/1‑139 (MQ=255)                                    aaaaaTGAGGGTGCCCAGAAAATGTATGTCGAGTTGCCAGCCATCATTATTCAACAGATTAATGCCGGCAGATATTTTTCGGTAAGGAGAAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                                   <  2:1450757/139‑1 (MQ=255)                                      aaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcccc                                                                                                 <  1:1035338/139‑1 (MQ=255)                                         tGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgc                                                                                              >  2:701132/1‑139 (MQ=255)                                             ggAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATACTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGAt                                                                                          >  2:374551/1‑139 (MQ=255)                                                 cccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                              <  2:267580/115‑1 (MQ=255)                                                 cccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                              >  1:267580/1‑115 (MQ=255)                                                                    cTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTCAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCa                                                                                                                   >  2:1148147/1‑91 (MQ=255)                                                                    cTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCa                                                                                                                   <  1:1148147/91‑1 (MQ=255)                                                                     ttGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg                                                                  <  2:58846/139‑1 (MQ=255)                                                                      tGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCgg                                                                 >  2:1666386/1‑139 (MQ=255)                                                                         cagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCa                                                              <  1:280201/139‑1 (MQ=255)                                                                          agccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt                                                             <  1:1064934/139‑1 (MQ=255)                                                                                     ttCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCAcc                                                  <  1:208063/139‑1 (MQ=255)                                                                                      tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGt                                                                                                                     <  1:457598/71‑1 (MQ=255)                                                                                      tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGt                                                                                                                     >  2:457598/1‑71 (MQ=255)                                                                                      tCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCa                                                 >  2:163678/1‑139 (MQ=255)                                                                                          cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc                                             >  1:1133707/1‑139 (MQ=255)                                                                                           aGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                            <  2:1276077/139‑1 (MQ=255)                                                                                           aGATTAATGACGGCAGATATTGATCGGTAAGGAGGAAATGCCGAGTTATTCGTGACGTTCAATGGTCAATGCCACACCCTGGACCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCTGGCATTACGTTTCACCATTTCa                                            <  2:44665/139‑1 (MQ=255)                                                                                             aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCa                                                                                                                          >  2:507931/1‑59 (MQ=255)                                                                                             aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCa                                                                                                                          <  1:507931/59‑1 (MQ=255)                                                                                             aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                              <  2:1645/71‑1 (MQ=255)                                                                                             aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                              >  1:1645/1‑71 (MQ=255)                                                                                             aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                             <  1:11206/72‑1 (MQ=255)                                                                                             aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                             >  2:11206/1‑72 (MQ=255)                                                                                             aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg                                          >  1:159856/1‑139 (MQ=255)                                                                                             aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg                                          >  2:1073475/1‑139 (MQ=255)                                                                                               tAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGaa                                        <  2:1133707/139‑1 (MQ=255)                                                                                                    aCGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGGTACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                            <  2:134244/130‑1 (MQ=255)                                                                                                    aCGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                            >  1:134244/1‑130 (MQ=255)                                                                                                      ggCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt                                                                     >  1:457056/1‑103 (MQ=255)                                                                                                      ggCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt                                                                     <  2:457056/103‑1 (MQ=255)                                                                                                       gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTc                                                                                                                    >  2:604832/1‑55 (MQ=255)                                                                                                       gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTc                                                                                                                    <  1:604832/55‑1 (MQ=255)                                                                                                       gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt                                                             <  2:1118846/110‑1 (MQ=255)                                                                                                       gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt                                                             >  1:1118846/1‑110 (MQ=255)                                                                                                       gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCa                                                 >  2:110349/1‑122 (MQ=255)                                                                                                       gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCa                                                 <  2:732109/122‑1 (MQ=255)                                                                                                       gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCa                                                 <  1:110349/122‑1 (MQ=255)                                                                                                       gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCa                                                 >  1:732109/1‑122 (MQ=255)                                                                                                       gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa                                >  2:1722732/1‑139 (MQ=255)                                                                                                         aGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAc                              <  1:163678/139‑1 (MQ=255)                                                                                                         aGATATTTATCGGAAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGAGTTGCCAGTCCTTTGCGGGCATTAAGTTTCACCATTTCATGGACCAGAGAAAc                              >  1:659800/1‑139 (MQ=255)                                                                                                                tATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg                                                                  <  1:1516778/96‑1 (MQ=255)                                                                                                                tATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg                                                                  >  2:1516778/1‑96 (MQ=255)                                                                                                                   cGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCa                                                 <  1:699171/110‑1 (MQ=255)                                                                                                                   cGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCa                                                 >  2:699171/1‑110 (MQ=255)                                                                                                                      tAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCaa                 >  1:1109088/1‑139 (MQ=255)                                                                                                                             gaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCTGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg          <  1:1751132/139‑1 (MQ=255)                                                                                                                             gaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg          <  2:438539/139‑1 (MQ=255)                                                                                                                             gaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg          <  1:288719/139‑1 (MQ=255)                                                                                                                                 tGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGACTCGGCAACCGGAAGCGcc      <  2:1181600/139‑1 (MQ=255)                                                                                                                                    agagTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCa                                                                            >  1:1561773/1‑66 (MQ=255)                                                                                                                                    agagTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCa                                                                            <  2:1561773/66‑1 (MQ=255)                                                                                                                                     gagTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATc  >  1:709292/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        GTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATC  >  NC_000913/2984276‑2984546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑