Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,065	G→A	*351* (TAG→TAA)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,065	0	G	A	100.0%	210.5 / NA	65	*351* (TAG→TAA)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/33);  total (32/33)

TCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCT  >  NC_000913/3103930‑3104195                                                                                                                                        |                                                                                                                                   tCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAacgc                                                                                                                                 >  2:1292473/1‑139 (MQ=255)  cccGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAAcgcg                                                                                                                                <  1:162589/139‑1 (MQ=255)  cccGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAAcgcg                                                                                                                                >  2:292974/1‑139 (MQ=255)    cGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTg                                                                                                                              >  2:1423475/1‑139 (MQ=255)           tcATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGAt                                                                                                                       <  2:1306701/139‑1 (MQ=255)            cATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATa                                                                                                                      <  1:1773703/139‑1 (MQ=255)             aTTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATaa                                                                                                                     >  1:1778649/1‑139 (MQ=255)               tCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAag                                                                                                                   <  2:889820/139‑1 (MQ=255)                    ggCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              <  1:198850/139‑1 (MQ=255)                         cATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGAttt                                                                                                         >  1:1545903/1‑139 (MQ=255)                                   cgCAATGCGCCCTGGTATAACTTAGCGCACCCGCCGCCAGTTGGCCTAGCGGCTCCCGTCGAACGATTGTTACAGCAGTTACGCACTGGCGCGCCTGATACACGCGTGACGCGATAAACGAGAAGACTAATGAACAGaa                                                                                               >  1:1120033/2‑139 (MQ=255)                                        tGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGAtt                                                                                          >  1:873953/1‑139 (MQ=255)                                        tGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGAtt                                                                                          >  2:505385/1‑139 (MQ=255)                                              ctGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              <  2:1402140/113‑1 (MQ=255)                                              ctGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              >  1:1402140/1‑113 (MQ=255)                                               tGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   <  1:292974/139‑1 (MQ=255)                                               tGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   <  1:111206/139‑1 (MQ=255)                                                 gTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACt                                                                                 <  1:1292473/139‑1 (MQ=255)                                                  tataACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGAt                                                                                                                       >  2:1160736/1‑100 (MQ=255)                                                  tataACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGAt                                                                                                                       <  1:1160736/100‑1 (MQ=255)                                                      aCTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCt                                                                            >  1:1709481/1‑139 (MQ=255)                                                       cTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTg                                                                           <  2:873953/139‑1 (MQ=255)                                                         tAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCa                                                                         >  1:604250/1‑139 (MQ=255)                                                          aGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCaa                                                                        <  2:1545903/139‑1 (MQ=255)                                                           gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATa                                                                                                                      <  1:414204/92‑1 (MQ=255)                                                           gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATa                                                                                                                      >  2:414204/1‑92 (MQ=255)                                                           gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              >  1:595466/1‑100 (MQ=255)                                                           gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              <  2:595466/100‑1 (MQ=255)                                                           gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   >  2:1402610/1‑127 (MQ=255)                                                           gcgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   <  1:1402610/127‑1 (MQ=255)                                                            cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   <  2:1619077/126‑1 (MQ=255)                                                            cgcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTa                                                                                   >  1:1619077/1‑126 (MQ=255)                                                             gcAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGt                                                                     <  2:1778649/139‑1 (MQ=255)                                                                 ccgccgTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGga                                                                                                               >  1:730483/1‑93 (MQ=255)                                                                 ccgccgTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGga                                                                                                               <  2:730483/93‑1 (MQ=255)                                                                                    cGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATaaa                                                                                                                    >  2:1267759/1‑69 (MQ=255)                                                                                    cGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATaaa                                                                                                                    <  1:1267759/69‑1 (MQ=255)                                                                                    cGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCa                                              >  2:1340324/1‑139 (MQ=255)                                                                                     ggCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGCTGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAg                                             <  1:842808/139‑1 (MQ=255)                                                                                     ggCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAg                                             <  2:1709481/139‑1 (MQ=255)                                                                                        tCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACg                                                                                             >  1:420862/1‑88 (MQ=255)                                                                                        tCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACg                                                                                             <  2:420862/88‑1 (MQ=255)                                                                                               gAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCg                                   >  1:908187/1‑139 (MQ=255)                                                                                                    tttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGa                                                                                                                             >  1:1443145/1‑44 (MQ=255)                                                                                                    tttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGa                                                                                                                             <  2:1443145/44‑1 (MQ=255)                                                                                                    tttGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAg                              <  1:1423475/139‑1 (MQ=255)                                                                                                          aCAGCAGTTACGCACTGGCGCGCCTGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCgg                                  <  2:1151840/129‑1 (MQ=255)                                                                                                          aCAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTg                                                                                     >  2:929954/1‑78 (MQ=255)                                                                                                          aCAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTg                                                                                     <  1:929954/78‑1 (MQ=255)                                                                                                          aCAGCAGTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCgg                                  >  1:1151840/1‑129 (MQ=255)                                                                                                            agcagTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGaaa                      >  2:899879/1‑139 (MQ=255)                                                                                                              cagTTACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAAc                    >  2:1522070/1‑139 (MQ=255)                                                                                                                 ttACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCa                 >  1:165867/1‑139 (MQ=255)                                                                                                                 ttACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCa                 >  1:1545125/1‑139 (MQ=255)                                                                                                                  tACGCACTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCAt                <  2:685241/139‑1 (MQ=255)                                                                                                                        cTGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATaa          >  2:1355482/1‑139 (MQ=255)                                                                                                                         tGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTc                                                        <  1:1124169/92‑1 (MQ=255)                                                                                                                         tGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAAc         <  2:911509/139‑1 (MQ=255)                                                                                                                         tGGCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAAc         <  1:1340324/139‑1 (MQ=255)                                                                                                                         tGGCGCGCCGGTTTAACCCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTc                                                        >  2:1124169/1‑92 (MQ=255)                                                                                                                          ggCGCGCCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACg        >  2:804764/1‑139 (MQ=255)                                                                                                                           gcgcgcCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              >  2:143555/1‑36 (MQ=255)                                                                                                                           gcgcgcCGGTTTAACGCGTGAGTCGATAAAGAGgat                                                                                                              <  1:143555/36‑1 (MQ=255)                                                                                                                           gcgcgcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACga       >  2:477869/1‑139 (MQ=255)                                                                                                                            cgcgcCGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACgag      <  2:604250/139‑1 (MQ=255)                                                                                                                                ccGGTTTAACGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGAtct  <  1:899879/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   TCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCTAGCGGCTCCCGTGGAGCGTTTGTTACAGCAGTTACGCACTGGCGCGCCGGTTTAGCGCGTGAGTCGATAAAGAGGATGATTTATGAGCAGAACGATTTTTTGTACTTTCCTGCAACGTGAAGCAGAAGGTCAGGATTTTCAGCTGTACCCCGGCGAGCTGGGAAAACGCATCTATAACGAGATCT  >  NC_000913/3103930‑3104195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑